BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20144 (507 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch... 29 0.40 SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 27 2.1 SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharo... 27 2.1 SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces... 26 2.8 SPCC70.09c |mug9||conserved fungal protein|Schizosaccharomyces p... 26 2.8 SPBC409.08 |||spermine family transporter |Schizosaccharomyces p... 25 6.5 SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1... 25 8.6 >SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 29.1 bits (62), Expect = 0.40 Identities = 11/21 (52%), Positives = 16/21 (76%) Frame = +2 Query: 101 VQTTVKKIHAYSHHLFALRTL 163 ++ VKKIH ++H +F LRTL Sbjct: 63 LKVAVKKIHPFNHPVFCLRTL 83 >SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.6 bits (56), Expect = 2.1 Identities = 21/76 (27%), Positives = 34/76 (44%) Frame = +1 Query: 4 TRNVFKNFKMKTLIFIMLVACVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHA 183 +RN F+ FK ++ +AS G + D+ + + PPL PK+ HR A Sbjct: 298 SRN-FEKFKKSNKKTVLFTTDIASR--GLDIPNVDFVLQ---LDPPLD-PKSFSHRCGRA 350 Query: 184 GKCACCPACVTLLGEG 231 G+ + LL +G Sbjct: 351 GRAGRAGVAIVLLNDG 366 >SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 26.6 bits (56), Expect = 2.1 Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 5/30 (16%) Frame = +2 Query: 80 RTVLWYVVQTTV-----KKIHAYSHHLFAL 154 R + WY + TT+ + I+AY HHLF + Sbjct: 109 RFINWYFLMTTLYYAYGESIYAYFHHLFIM 138 >SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 796 Score = 26.2 bits (55), Expect = 2.8 Identities = 17/47 (36%), Positives = 24/47 (51%) Frame = -3 Query: 406 RTQIIFRTDRDRSIASLYFPANENDYTSLVQTLLMHLRGSLHTAEGV 266 R I+ TDRD ++ S F +Y SLV L+G L A+G+ Sbjct: 569 RDDRIYLTDRDMNVVSYSFNLAIIEYQSLV------LKGDLEAAQGL 609 >SPCC70.09c |mug9||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 208 Score = 26.2 bits (55), Expect = 2.8 Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 1/63 (1%) Frame = -3 Query: 379 RDRSIASLYFPANENDYTSLVQTLLMHLRGSLHTAEGVSPSSSNKSCKL-HLHQVKLHKR 203 RD S S+ P N + L +R AEG P+S+ K+ ++ +L V KR Sbjct: 39 RDYSGRSICQPDTNNPTRWKYERPLETIRAWQDVAEGKVPASNEKAARVSNLKTVPSLKR 98 Query: 202 DNK 194 +NK Sbjct: 99 ENK 101 >SPBC409.08 |||spermine family transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 539 Score = 25.0 bits (52), Expect = 6.5 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = -3 Query: 289 SLHTAEGVSPSSSNKSCKLHLHQVKLHKRDNKHTFQRVWR 170 +L TAEGV + + L +L+ +N H + R+W+ Sbjct: 61 NLETAEGVYRDPTINEGEEELVTWELNDPENPHNWSRLWK 100 >SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1 complex subunit Tpr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1039 Score = 24.6 bits (51), Expect = 8.6 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -2 Query: 173 ARCSVFLGQTSGGCMHGFFSQ*SVPHTKAPY 81 ARC F+ Q SGG + GF + + + K Y Sbjct: 161 ARCFDFVLQKSGGNILGFLGKARILYAKGNY 191 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,110,059 Number of Sequences: 5004 Number of extensions: 42688 Number of successful extensions: 122 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 116 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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