SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20144
         (507 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch...    29   0.40 
SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy...    27   2.1  
SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharo...    27   2.1  
SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces...    26   2.8  
SPCC70.09c |mug9||conserved fungal protein|Schizosaccharomyces p...    26   2.8  
SPBC409.08 |||spermine family transporter |Schizosaccharomyces p...    25   6.5  
SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1...    25   8.6  

>SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 372

 Score = 29.1 bits (62), Expect = 0.40
 Identities = 11/21 (52%), Positives = 16/21 (76%)
 Frame = +2

Query: 101 VQTTVKKIHAYSHHLFALRTL 163
           ++  VKKIH ++H +F LRTL
Sbjct: 63  LKVAVKKIHPFNHPVFCLRTL 83


>SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 606

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 21/76 (27%), Positives = 34/76 (44%)
 Frame = +1

Query: 4   TRNVFKNFKMKTLIFIMLVACVASAAYGALVCGTDYCEKNPCIQPPLVCPKNTEHRARHA 183
           +RN F+ FK      ++    +AS   G  +   D+  +   + PPL  PK+  HR   A
Sbjct: 298 SRN-FEKFKKSNKKTVLFTTDIASR--GLDIPNVDFVLQ---LDPPLD-PKSFSHRCGRA 350

Query: 184 GKCACCPACVTLLGEG 231
           G+       + LL +G
Sbjct: 351 GRAGRAGVAIVLLNDG 366


>SPBC13A2.03 |||phosphatidate
           cytidylyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 439

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 5/30 (16%)
 Frame = +2

Query: 80  RTVLWYVVQTTV-----KKIHAYSHHLFAL 154
           R + WY + TT+     + I+AY HHLF +
Sbjct: 109 RFINWYFLMTTLYYAYGESIYAYFHHLFIM 138


>SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 796

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 17/47 (36%), Positives = 24/47 (51%)
 Frame = -3

Query: 406 RTQIIFRTDRDRSIASLYFPANENDYTSLVQTLLMHLRGSLHTAEGV 266
           R   I+ TDRD ++ S  F     +Y SLV      L+G L  A+G+
Sbjct: 569 RDDRIYLTDRDMNVVSYSFNLAIIEYQSLV------LKGDLEAAQGL 609


>SPCC70.09c |mug9||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 208

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
 Frame = -3

Query: 379 RDRSIASLYFPANENDYTSLVQTLLMHLRGSLHTAEGVSPSSSNKSCKL-HLHQVKLHKR 203
           RD S  S+  P   N      +  L  +R     AEG  P+S+ K+ ++ +L  V   KR
Sbjct: 39  RDYSGRSICQPDTNNPTRWKYERPLETIRAWQDVAEGKVPASNEKAARVSNLKTVPSLKR 98

Query: 202 DNK 194
           +NK
Sbjct: 99  ENK 101


>SPBC409.08 |||spermine family transporter |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 539

 Score = 25.0 bits (52), Expect = 6.5
 Identities = 12/40 (30%), Positives = 21/40 (52%)
 Frame = -3

Query: 289 SLHTAEGVSPSSSNKSCKLHLHQVKLHKRDNKHTFQRVWR 170
           +L TAEGV    +    +  L   +L+  +N H + R+W+
Sbjct: 61  NLETAEGVYRDPTINEGEEELVTWELNDPENPHNWSRLWK 100


>SPAC27D7.14c |tpr1|SPAC637.02c|RNA polymerase II associated Paf1
           complex subunit Tpr1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1039

 Score = 24.6 bits (51), Expect = 8.6
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -2

Query: 173 ARCSVFLGQTSGGCMHGFFSQ*SVPHTKAPY 81
           ARC  F+ Q SGG + GF  +  + + K  Y
Sbjct: 161 ARCFDFVLQKSGGNILGFLGKARILYAKGNY 191


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,110,059
Number of Sequences: 5004
Number of extensions: 42688
Number of successful extensions: 122
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 202220600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -