BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20143 (621 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02170.1 68416.m00190 expressed protein 30 1.4 At2g16750.1 68415.m01921 protein kinase family protein contains ... 28 4.3 At3g31370.1 68416.m03999 hypothetical protein contains similarit... 28 5.7 At5g01990.1 68418.m00118 auxin efflux carrier family protein con... 27 7.6 >At3g02170.1 68416.m00190 expressed protein Length = 905 Score = 29.9 bits (64), Expect = 1.4 Identities = 23/87 (26%), Positives = 40/87 (45%), Gaps = 2/87 (2%) Frame = +1 Query: 232 LLKIQCSKISFQIAQ**I*SNVNIFILIQQNGSKNSFTLEGIWNSIMCDPHYCNSRYLLR 411 L ++ S ISFQ+ Q + N +F +++QN + N + N +R Sbjct: 735 LRDLEYSMISFQLHQTRLPINPGLFFILEQNKASNVTLPDNKHRGRGFRQQQTNPTETIR 794 Query: 412 LAVSYSTVS*I-RRKFNTNNCL-PRIM 486 + + TV+ I RKF C+ PR++ Sbjct: 795 RKLVFDTVNEILARKFTAEGCIKPRLI 821 >At2g16750.1 68415.m01921 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 617 Score = 28.3 bits (60), Expect = 4.3 Identities = 13/31 (41%), Positives = 21/31 (67%) Frame = -1 Query: 105 TKRYNR*GFILEGLCIVGLLVHDARRTRAMS 13 TK+ G +L+G ++G+LV +A+R AMS Sbjct: 74 TKKIELKGEVLKGNSVLGVLVKEAKRYNAMS 104 >At3g31370.1 68416.m03999 hypothetical protein contains similarity to hypothetical proteins Length = 272 Score = 27.9 bits (59), Expect = 5.7 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = +1 Query: 331 KNSFTLEGIWNSIMCDPHYCNSRYL 405 K TLE +W +M D +C+S L Sbjct: 125 KTKLTLEHVWREVMFDQKWCSSNAL 149 >At5g01990.1 68418.m00118 auxin efflux carrier family protein contains auxin efflux carrier domain, Pfam:PF03547 Length = 431 Score = 27.5 bits (58), Expect = 7.6 Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 2/44 (4%) Frame = +2 Query: 440 ESEESSIPTTAYQ--GSWIVSIMILARL*HRFHPPISPTNSEEK 565 +SE+ SI TAY G W+ +I++ + F PP ++EE+ Sbjct: 158 DSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEE 201 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,266,008 Number of Sequences: 28952 Number of extensions: 197412 Number of successful extensions: 418 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 411 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 418 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1255974912 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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