Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20135
(661 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g27710.1 68416.m03460 zinc finger protein-related contains si... 33 0.13
At3g27720.1 68416.m03461 zinc finger protein-related contains Pf... 32 0.29
At4g34370.1 68417.m04883 IBR domain-containing protein similar t... 31 0.90
At1g75960.1 68414.m08822 AMP-binding protein, putative similar t... 30 1.6
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot... 29 2.1
At4g35000.1 68417.m04963 L-ascorbate peroxidase 3 (APX3) identic... 29 2.7
At1g52320.2 68414.m05905 expressed protein contains Pfam profile... 29 2.7
At1g52320.1 68414.m05904 expressed protein contains Pfam profile... 29 2.7
At5g63450.1 68418.m07965 cytochrome P450, putative 28 4.8
At4g31110.1 68417.m04415 wall-associated kinase, putative simila... 28 4.8
At4g31100.1 68417.m04414 wall-associated kinase, putative 28 4.8
At4g13040.1 68417.m02035 AP2 domain-containing transcription fac... 28 4.8
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 28 4.8
At1g19390.1 68414.m02412 wall-associated kinase, putative simila... 28 6.3
At1g59980.1 68414.m06757 DNAJ heat shock N-terminal domain-conta... 27 8.4
>At3g27710.1 68416.m03460 zinc finger protein-related contains
similarity to zinc finger proteins and Pfam domain,
PF01485: IBR domain
Length = 537
Score = 33.5 bits (73), Expect = 0.13
Identities = 18/62 (29%), Positives = 33/62 (53%)
Frame = +3
Query: 18 KLEDDLYNSILVADYDNAVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYAYQLWMQG 197
KLED L SIL N+ K ++++ K+ +T+ VN+L + ++ Y + +M G
Sbjct: 363 KLEDKLRKSILEKAVSNSETKDQKVF--KEYSWVTDAVNRLFISRRILSQSYPFAFYMFG 420
Query: 198 SK 203
+
Sbjct: 421 EE 422
>At3g27720.1 68416.m03461 zinc finger protein-related contains
Pfam:PF01485 IBR domain
Length = 493
Score = 32.3 bits (70), Expect = 0.29
Identities = 19/62 (30%), Positives = 33/62 (53%)
Frame = +3
Query: 18 KLEDDLYNSILVADYDNAVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYAYQLWMQG 197
KLED L SIL N+ K ++++ K+ I + VN+L R+ ++ Y + +M G
Sbjct: 323 KLEDKLKKSILKKAVLNSETKDQKVF--KEYSWIIDAVNRLFRSRRILSYSYPFVFYMFG 380
Query: 198 SK 203
+
Sbjct: 381 KE 382
>At4g34370.1 68417.m04883 IBR domain-containing protein similar to
SP|Q94981 Ariadne-1 protein (Ari-1) {Drosophila
melanogaster}; contains Pfam profile PF01485: IBR domain
Length = 597
Score = 30.7 bits (66), Expect = 0.90
Identities = 20/73 (27%), Positives = 37/73 (50%)
Frame = +3
Query: 15 SKLEDDLYNSILVADYDNAVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYAYQLWMQ 194
SKLED L ++I ++ + K+ + K +TN +++L R+ ++ YA+ +M
Sbjct: 364 SKLEDKLRDTI----HEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMF 419
Query: 195 GSKDIVRECFPVE 233
G + E P E
Sbjct: 420 GEEMFKDEMTPEE 432
>At1g75960.1 68414.m08822 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam profile: PF00501 AMP-binding enzyme;
identical to cDNA adenosine monophosphate binding
protein 8 AMPBP8 (AMPBP8) GI:20799724
Length = 544
Score = 29.9 bits (64), Expect = 1.6
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = +3
Query: 564 VLHVQPRINEALVLSKPTDTWG 629
VL+ P +NEA V+++P + WG
Sbjct: 458 VLYTNPAVNEAAVVARPDEFWG 479
>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor [Homo
sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1706
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +1
Query: 442 VFDVVS*NNPDPKPHGLRGQQRQGFKAQWTLQPAKYDN 555
+ ++VS PD KPHG G+ + + Q+ +P K ++
Sbjct: 770 ILNLVSWMQPDEKPHGANGRLIRDIQEQFQAKPEKSES 807
>At4g35000.1 68417.m04963 L-ascorbate peroxidase 3 (APX3) identical
to ascorbate peroxidase 3 [Arabidopsis thaliana]
GI:2444019, L-ascorbate peroxidase [Arabidopsis
thaliana] gi|1523791|emb|CAA66926; similar to ascorbate
peroxidase [Gossypium hirsutum] gi|1019946|gb|AAB52954
Length = 287
Score = 29.1 bits (62), Expect = 2.7
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 502 QRQGFKAQWTLQPAKYDNDVLFFMYNRES 588
+R GF WT +P K+DN + ES
Sbjct: 168 ERSGFDGPWTQEPLKFDNSYFVELLKGES 196
>At1g52320.2 68414.m05905 expressed protein contains Pfam profile:
PF04782 protein of unknown function (DUF632)
Length = 398
Score = 29.1 bits (62), Expect = 2.7
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = -1
Query: 349 LAIAVGKSAIVVAVIAQRQSKTVALVHQLNVVSAKISVNSTGKHSRTISLEPCIQSW 179
L A+GK ++ + QRQ++ ++ L+V A N H RTI L +Q W
Sbjct: 122 LVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDH-HHERTIQLLAVVQEW 177
>At1g52320.1 68414.m05904 expressed protein contains Pfam profile:
PF04782 protein of unknown function (DUF632)
Length = 398
Score = 29.1 bits (62), Expect = 2.7
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = -1
Query: 349 LAIAVGKSAIVVAVIAQRQSKTVALVHQLNVVSAKISVNSTGKHSRTISLEPCIQSW 179
L A+GK ++ + QRQ++ ++ L+V A N H RTI L +Q W
Sbjct: 122 LVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDH-HHERTIQLLAVVQEW 177
>At5g63450.1 68418.m07965 cytochrome P450, putative
Length = 510
Score = 28.3 bits (60), Expect = 4.8
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +2
Query: 344 GKDKTSPKVSWKFVPLWENNKVYFKIVNTQRNQ 442
G+D TS ++W F L +N+ V KI++ RN+
Sbjct: 306 GRDTTSAAMTWLFWLLSQNDDVETKILDELRNK 338
>At4g31110.1 68417.m04415 wall-associated kinase, putative similar
to wall-associated kinase 1, Arabidopsis thaliana,
gb:AJ009696
Length = 756
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = +3
Query: 33 LYNSILVADYDNAVEKSKQIYEDKKSEVITNVV 131
+Y +LV AV+KSK I EDK E I VV
Sbjct: 430 VYKGMLVDGRTVAVKKSKVIDEDKLQEFINEVV 462
>At4g31100.1 68417.m04414 wall-associated kinase, putative
Length = 786
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = +3
Query: 33 LYNSILVADYDNAVEKSKQIYEDKKSEVITNVV 131
+Y +LV AV+KSK I EDK E I VV
Sbjct: 458 VYKGMLVDGRTVAVKKSKVIDEDKLQEFINEVV 490
>At4g13040.1 68417.m02035 AP2 domain-containing transcription factor
family protein contains Pfam profile PF00847: AP2 domain
Length = 226
Score = 28.3 bits (60), Expect = 4.8
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +1
Query: 10 PTPNSKTIFTTASSLPITTMPLKKANRSTRTRRAKSSQMS*TNSY 144
P PN++ + + +TT+ ++K R+RR +S M + Y
Sbjct: 36 PNPNARAAYNPGPAETVTTVIVEKKAIEERSRRTRSKHMHFRSDY 80
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 28.3 bits (60), Expect = 4.8
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +3
Query: 564 VLHVQPRINEALVLSKPTDTWG 629
VL+ P I EA V++KP WG
Sbjct: 459 VLYTNPAIKEAAVVAKPDKMWG 480
>At1g19390.1 68414.m02412 wall-associated kinase, putative similar
to GB:CAB42872 from [Arabidopsis thaliana] (Plant Mol.
Biol. 39 (6), 1189-1196 (1999))
Length = 788
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +3
Query: 33 LYNSILVADYDNAVEKSKQIYEDKKSEVITNVV 131
+Y +LV AV+KSK + EDK E I VV
Sbjct: 465 VYKGMLVDGRTVAVKKSKVVDEDKLEEFINEVV 497
>At1g59980.1 68414.m06757 DNAJ heat shock N-terminal
domain-containing protein similar to Altered Response to
Gravity [Arabidopsis thaliana] GI:4249662; contains Pfam
profile PF00226 DnaJ domain
Length = 414
Score = 27.5 bits (58), Expect = 8.4
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 4/42 (9%)
Frame = +2
Query: 221 LPC*VHTYFRRNNIKLMY----KRDGLALTLRDDSNNDGRLA 334
L C VH+ +N KL+Y + GL+L L++DS G+L+
Sbjct: 177 LICKVHSS-AKNKFKLLYFDQVENGGLSLALQEDSRKTGKLS 217
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,384,886
Number of Sequences: 28952
Number of extensions: 227532
Number of successful extensions: 807
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 785
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 807
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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