BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20132 (714 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC646.05c |erg9||squalene synthase Erg9|Schizosaccharomyces po... 31 0.12 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 30 0.38 SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 28 1.2 SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|... 26 4.7 SPCC645.04 |nse3||Smc5-6 complex non-SMC subunit Nse3 |Schizosac... 26 6.1 >SPBC646.05c |erg9||squalene synthase Erg9|Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 31.5 bits (68), Expect = 0.12 Identities = 24/71 (33%), Positives = 38/71 (53%), Gaps = 2/71 (2%) Frame = +3 Query: 285 TSRN--IFLEALPHARSEACARFIKYLVIEEKDKIEDAALLSLIRKLPFNVATFDQSLLE 458 TSR+ + ++ LP+ EA F YLV+ D +ED L L +KLP + F +++ Sbjct: 45 TSRSFAVVIKELPNGIREAVMIF--YLVLRGLDTVEDDMTLPLDKKLPI-LRDFYKTIEV 101 Query: 459 ELEAFTKLGPD 491 E F + GP+ Sbjct: 102 EGWTFNESGPN 112 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 29.9 bits (64), Expect = 0.38 Identities = 15/39 (38%), Positives = 25/39 (64%) Frame = -3 Query: 679 IAYVAQTLEPHHPVQVLRAIXIHSEIFHDYIVNVVLFDK 563 I+ V Q LEP HP ++ + I IH E+ D + ++V F++ Sbjct: 2380 ISSVGQ-LEPWHPTELDQKIRIHPEVKVDSLTSLVTFNE 2417 >SPAC328.04 |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 741 Score = 28.3 bits (60), Expect = 1.2 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = -3 Query: 649 HHPVQVLRAIXIHSEIFHDYIVNVVLFDK*KFNCLM 542 ++ Q A+ IH E DY+ + LF K K N LM Sbjct: 7 YNVTQSYAAVAIHKEQTADYVEAIALFQKAKRNILM 42 >SPAC2G11.12 |rqh1|hus2, rad12, rec9|RecQ type DNA helicase Rqh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1328 Score = 26.2 bits (55), Expect = 4.7 Identities = 15/41 (36%), Positives = 20/41 (48%) Frame = +3 Query: 387 DAALLSLIRKLPFNVATFDQSLLEELEAFTKLGPDFTAEIR 509 D+ LLS+ +KLP NV + E LGP F I+ Sbjct: 1146 DSVLLSMAKKLPRNVKELKEIHGVSNEKAVNLGPKFLQVIQ 1186 >SPCC645.04 |nse3||Smc5-6 complex non-SMC subunit Nse3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 25.8 bits (54), Expect = 6.1 Identities = 18/81 (22%), Positives = 35/81 (43%), Gaps = 1/81 (1%) Frame = +3 Query: 189 LYRVVEHYSPAELHDHH*AVRRLVLGTSYDMETSRNIFLEALPHARSEACARF-IKYLVI 365 L R V Y+ H+ R+ ++ ++ TSRN+F A + F + + + Sbjct: 93 LVRNVVRYAICSQTSHNTITRKDIVQKAFPEGTSRNLFQSVFEEADRQLQLSFGFRLVAV 152 Query: 366 EEKDKIEDAALLSLIRKLPFN 428 + ++ +D A+ L R N Sbjct: 153 TQSNRKKDMAVSQLRRPATSN 173 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,979,800 Number of Sequences: 5004 Number of extensions: 62549 Number of successful extensions: 161 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 159 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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