Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20125
(641 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35) 57 1e-08
SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.) 55 6e-08
SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.) 53 2e-07
SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06
SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35) 45 6e-05
SB_23671| Best HMM Match : Tetraspannin (HMM E-Value=6.6e-38) 43 2e-04
SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0) 40 0.002
SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43) 40 0.002
SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.) 36 0.021
SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0) 36 0.028
SB_22249| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.11
SB_31427| Best HMM Match : Tetraspannin (HMM E-Value=0) 33 0.15
SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06) 32 0.34
SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09) 30 1.4
SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.4
SB_57558| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.6
SB_38522| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.6
SB_38027| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.6
SB_43317| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.4
SB_42994| Best HMM Match : NHL (HMM E-Value=8.4e-34) 28 7.4
SB_15163| Best HMM Match : VAR1 (HMM E-Value=3.1) 28 7.4
SB_31699| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.8
SB_12405| Best HMM Match : I-set (HMM E-Value=8.8e-21) 27 9.8
SB_3828| Best HMM Match : Tetraspannin (HMM E-Value=6.7e-08) 27 9.8
>SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35)
Length = 642
Score = 57.2 bits (132), Expect = 1e-08
Identities = 26/69 (37%), Positives = 41/69 (59%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 478
CCGA KEN CM+I F + LLI+ E+A + GY+ ++ + ++ +I +YP KN
Sbjct: 425 CCGAWKENKCMLILFFILLLIVLGLEIAAAVLGYINKDKIQSDLTDDIIRNIKEYP--KN 482
Query: 479 VQKTIDIIQ 505
+ ID +Q
Sbjct: 483 NKAAIDAMQ 491
Score = 28.3 bits (60), Expect = 5.6
Identities = 15/46 (32%), Positives = 21/46 (45%), Gaps = 4/46 (8%)
Frame = +1
Query: 508 NLQCCGINSPADWADHG----LPIPSTCCSAQEINDGVVAACTENS 633
+ +CCG P+DW +G P +CC ND + T NS
Sbjct: 493 DFECCGAKGPSDWTFYGQMKYKAGPGSCCGKPN-NDACLPG-TRNS 536
>SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 341
Score = 54.8 bits (126), Expect = 6e-08
Identities = 27/70 (38%), Positives = 43/70 (61%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 478
CCGA KEN CM+I F FLL++ + E+ G Y +E+ + +++ +I YP KN
Sbjct: 72 CCGAWKENKCMLICFFAFLLLLLILEIVGGALAYNNKDKIENRLDKDILKAIENYP-GKN 130
Query: 479 VQKTIDIIQT 508
+K+I+ +QT
Sbjct: 131 -EKSINDMQT 139
Score = 38.3 bits (85), Expect = 0.005
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = +3
Query: 84 MALEGGMSCVKYLMFCFNLLFAITGLIILIVGIRAEINSYPYMNFTDENF 233
MAL+G + VK L+ FN +F + GLI+ VGI A Y+ N+
Sbjct: 1 MALQGSSNIVKILVIIFNFIFFLFGLILFGVGIWASTKLGAYVEIASVNY 50
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/38 (36%), Positives = 24/38 (63%), Gaps = 5/38 (13%)
Frame = +1
Query: 511 LQCCGINSPADW--ADH-GLP--IPSTCCSAQEINDGV 609
++CCGI + DW +D+ GL +P +CC+ E + G+
Sbjct: 249 VKCCGITTYEDWKSSDNLGLTDNVPDSCCTTVEKDCGM 286
>SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 333
Score = 52.8 bits (121), Expect = 2e-07
Identities = 24/69 (34%), Positives = 43/69 (62%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 478
C GA++EN C++ FSV L IIF +LA+G+ ++ +E ++ L ++T+Y + +
Sbjct: 65 CIGALRENICLLKFFSVVLAIIFFLQLALGVFVFVFQDKVEAVIVEKLQTAVTKYRDNAD 124
Query: 479 VQKTIDIIQ 505
+Q ID +Q
Sbjct: 125 LQNLIDGVQ 133
>SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 514
Score = 49.6 bits (113), Expect = 2e-06
Identities = 19/55 (34%), Positives = 32/55 (58%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY 463
CCGA+KE+ C++ TF L II E+ GY+ +E+ + +++N +I Y
Sbjct: 344 CCGAIKEHRCLLATFFALLFIILAVEITGTALGYVYRNKVEEQLGKDMNDTIVSY 398
>SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35)
Length = 286
Score = 44.8 bits (101), Expect = 6e-05
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 478
C GA+KEN + ++ +F+ + +A G+ +++ L D R L + Y D +
Sbjct: 91 CVGALKENTLFLASYGIFIFCCLIPIIAAGVLAFLQREKLVDPKAR-LEDGLKSYQQDSS 149
Query: 479 VQKTIDIIQT 508
Q+ ID IQT
Sbjct: 150 KQEFIDFIQT 159
>SB_23671| Best HMM Match : Tetraspannin (HMM E-Value=6.6e-38)
Length = 919
Score = 43.2 bits (97), Expect = 2e-04
Identities = 16/69 (23%), Positives = 37/69 (53%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 478
C +KEN +++++ + L++IF+ E+A G+ + +++ + + + +Y
Sbjct: 78 CVAVIKENRFILVSYLIMLVLIFILEIATGVVAVIYRSEISYELEVGIRGKMNRYGFTDA 137
Query: 479 VQKTIDIIQ 505
V K ID +Q
Sbjct: 138 VTKAIDDLQ 146
>SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0)
Length = 252
Score = 39.9 bits (89), Expect = 0.002
Identities = 19/70 (27%), Positives = 39/70 (55%), Gaps = 2/70 (2%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 478
CCGA+KE+ CM+ T+ + IF E+ + Y+ +E ++ ++ +++ +Y + +
Sbjct: 77 CCGALKESQCMLGTYFGLVTAIFCLEVTAIVLAYVYRERIEKNLHGDIRSTMNEYHLSGH 136
Query: 479 --VQKTIDII 502
V + ID I
Sbjct: 137 DAVTRAIDEI 146
Score = 39.5 bits (88), Expect = 0.002
Identities = 21/62 (33%), Positives = 34/62 (54%), Gaps = 5/62 (8%)
Frame = +3
Query: 84 MALEGGMSCVKYLMFCFNLLFAITGLIILIVGI-----RAEINSYPYMNFTDENFYKFAP 248
M+ + G C+K+++F FNLLF + G I +G+ + E+ + Y T YK AP
Sbjct: 1 MSTDWGFKCIKFMVFFFNLLFLLAGCAIFAIGVWIISSKDEV-AGDYSRLTGTINYKTAP 59
Query: 249 IV 254
I+
Sbjct: 60 IL 61
>SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43)
Length = 211
Score = 39.9 bits (89), Expect = 0.002
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGY 400
C GA KEN CM+ TF V LLI+ E+A + Y
Sbjct: 74 CLGAWKENRCMLGTFFVLLLILLTLEIAAAVLAY 107
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +3
Query: 96 GGMSCVKYLMFCFNLLFAITGLIILIVGIRAEI 194
G +K L+ FN+LF I G+ ++ VG AE+
Sbjct: 7 GAGRILKILVVFFNVLFFIFGIALIAVGAWAEV 39
>SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 313
Score = 36.3 bits (80), Expect = 0.021
Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 2/72 (2%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY--PVD 472
C GA++EN C++ + II + G G+ LE V L +I Y P
Sbjct: 122 CIGALRENRCLLTAYIWMCGIILAGMIVCGGLGFYYKDVLETKVTAQLKDAIVLYRDPTK 181
Query: 473 KNVQKTIDIIQT 508
++ ID +QT
Sbjct: 182 GDLHLIIDTVQT 193
Score = 33.5 bits (73), Expect = 0.15
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +3
Query: 108 CVKYLMFCFNLLFAITGLIILIVGI 182
CVKY +F N++F + G +IL VGI
Sbjct: 59 CVKYSLFTINVIFWLAGAVILAVGI 83
>SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0)
Length = 280
Score = 35.9 bits (79), Expect = 0.028
Identities = 18/70 (25%), Positives = 32/70 (45%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYPVDKN 478
C GA++EN C + + + + + +A G Y + D ++ L + +Y D
Sbjct: 96 CVGALRENTCFLTVYGTSVFLCLILLIACGAVAYTQ-KDKVQALKERLKQDLKKYLDDPL 154
Query: 479 VQKTIDIIQT 508
Q ID +QT
Sbjct: 155 NQDLIDFMQT 164
Score = 32.7 bits (71), Expect = 0.26
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +3
Query: 108 CVKYLMFCFNLLFAITGLIILIVGI 182
C KY +F N++F I GL+++ VG+
Sbjct: 27 CTKYTLFFINVIFFILGLLVMTVGV 51
>SB_22249| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 296
Score = 33.9 bits (74), Expect = 0.11
Identities = 21/72 (29%), Positives = 36/72 (50%), Gaps = 2/72 (2%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY--PVD 472
C GA++EN ++ + + + + ++ GIA YM +D V+ L + Y P
Sbjct: 100 CIGALRENTILLAIYGGAVTLCLIFLVSAGIAVYMH----QDKVVEKLKDDLKHYRDPDK 155
Query: 473 KNVQKTIDIIQT 508
+N Q ID +QT
Sbjct: 156 ENFQFLIDRLQT 167
Score = 33.5 bits (73), Expect = 0.15
Identities = 10/25 (40%), Positives = 21/25 (84%)
Frame = +3
Query: 105 SCVKYLMFCFNLLFAITGLIILIVG 179
+C+KY++F N++F + G++IL++G
Sbjct: 37 TCIKYILFFVNVVFWVFGVLILVIG 61
Score = 31.9 bits (69), Expect = 0.46
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +1
Query: 508 NLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENS 633
NL+CCG+NS DW + I C S + GV +C + S
Sbjct: 168 NLKCCGVNSYTDWQTN---IYFNCSSPGSESCGVPFSCCKES 206
>SB_31427| Best HMM Match : Tetraspannin (HMM E-Value=0)
Length = 299
Score = 33.5 bits (73), Expect = 0.15
Identities = 10/25 (40%), Positives = 21/25 (84%)
Frame = +3
Query: 105 SCVKYLMFCFNLLFAITGLIILIVG 179
+C+KY++F N++F + G++IL++G
Sbjct: 37 TCIKYILFFVNVVFWVFGVLILVIG 61
Score = 31.1 bits (67), Expect = 0.80
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +1
Query: 508 NLQCCGINSPADWADHGLPIPSTCCSAQEINDGVVAACTENS 633
NL+CCGINS DW + + C S + GV +C + S
Sbjct: 168 NLKCCGINSYNDWQTN---VYFNCSSPGSESCGVPFSCCKES 206
Score = 29.5 bits (63), Expect = 2.4
Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 2/72 (2%)
Frame = +2
Query: 299 CCGAVKENHCMIITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQY--PVD 472
C GA++EN ++ + + + + ++ G A Y+ D V+ L + Y P
Sbjct: 100 CIGALRENTILLAIYGGAVTLCLILLVSAGTAVYIH----RDKVVAKLKDDLQHYRDPDK 155
Query: 473 KNVQKTIDIIQT 508
+N Q ID +QT
Sbjct: 156 ENFQFLIDRLQT 167
>SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06)
Length = 187
Score = 32.3 bits (70), Expect = 0.34
Identities = 12/44 (27%), Positives = 25/44 (56%)
Frame = +2
Query: 335 ITFSVFLLIIFVAELAVGIAGYMKHTDLEDSVMRNLNASITQYP 466
+ F V L ++F+ EL I GY+ ++++ + L+ ++ YP
Sbjct: 130 LQFGVILGLLFLVELGTAITGYIFRSEIKTGLSDGLDTALKDYP 173
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +3
Query: 135 NLLFAITGLIILIVGIRAEINSYPYMNFTDENFYKFAPIV 254
+L+F + GL +L VGI A I YM + ++ A I+
Sbjct: 39 HLVFQLMGLSVLAVGIWARIQFSDYMKLSSHDYATAAYIL 78
>SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09)
Length = 669
Score = 30.3 bits (65), Expect = 1.4
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +3
Query: 99 GMSCVKYLMFCFNLLFAITGLI 164
GM C+KYL+F FN +F + +I
Sbjct: 76 GMQCMKYLLFAFNFVFWCSKII 97
Score = 29.1 bits (62), Expect = 3.2
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +2
Query: 299 CCGAVKENHCMIITFS 346
CCGAVKEN C ++ S
Sbjct: 217 CCGAVKENKCFLLLVS 232
>SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 426
Score = 29.5 bits (63), Expect = 2.4
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +2
Query: 299 CCGAVKENHCMIIT 340
CCGAVKE+ CM+ T
Sbjct: 332 CCGAVKESRCMLAT 345
>SB_57558| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 733
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -2
Query: 196 FISALMPTIRMINPVMANRRLKQNMRYF 113
FI+ALM I +I +M + L++ M YF
Sbjct: 39 FITALMGNIILITVIMKTKSLRRTMNYF 66
>SB_38522| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 839
Score = 28.3 bits (60), Expect = 5.6
Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = -1
Query: 188 SPDANYQDDQSRNGKQKIEAKHEVLYTG---HSTFESHC 81
SP ++ RNG+ K+E+ YTG H+ +SHC
Sbjct: 22 SPLHAHKQSHCRNGEPKVESNKVQYYTGSPLHAHKQSHC 60
>SB_38027| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 316
Score = 28.3 bits (60), Expect = 5.6
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +2
Query: 302 CGAVKENHCMIITFSVFLLIIFVAELAVGI 391
C + N C++ T+ F+LI+ VAE+ + +
Sbjct: 38 CALLYMNPCLLKTYFSFMLILVVAEVTIAV 67
>SB_43317| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 109
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/51 (23%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Frame = -1
Query: 209 VRIRVYFSPDANYQ---DDQSRNGKQKIEAKHEVLYTGHSTFESHCYSIKY 66
+ + +Y + ++ Y+ D+ R GK ++ +KH Y + + CY + Y
Sbjct: 56 LHLHLYDNRESEYRKRIDELEREGKWRLHSKHVTDYNKYIPARAKCYLLTY 106
>SB_42994| Best HMM Match : NHL (HMM E-Value=8.4e-34)
Length = 750
Score = 27.9 bits (59), Expect = 7.4
Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +2
Query: 41 SVVIQFGGNI*LNNNGSRRWNVLCKVPHVLLQSSVCHYGIDHPD-SWHQG*NKLVSLHEL 217
+V + GN+ + + GSR W++ +PH L IDH D +W V+LH++
Sbjct: 127 AVALDMRGNVVVFHRGSRAWDLKFYMPHGLT--------IDHEDNTWITD----VALHQV 174
Query: 218 HR 223
H+
Sbjct: 175 HK 176
>SB_15163| Best HMM Match : VAR1 (HMM E-Value=3.1)
Length = 354
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/43 (30%), Positives = 28/43 (65%), Gaps = 3/43 (6%)
Frame = +2
Query: 398 YMKHTDLEDSVMRNLNASITQYPVDKN-VQKTI--DIIQTTCN 517
+++HT +D + N+N + Q+ ++++ +Q TI D++Q T N
Sbjct: 142 FLQHTINQDLLQHNINQDLLQHTINQDLLQHTINQDLLQHTIN 184
>SB_31699| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 6119
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/57 (26%), Positives = 23/57 (40%)
Frame = +1
Query: 388 HRWLHEAYGLGRFGYAQPERFHYSISGRQERPEDY*YHTNNLQCCGINSPADWADHG 558
HR + Y GR+ + E + ++ R RPED QC +N + G
Sbjct: 2094 HRESEQIYDGGRYSIVEEENGLFVLTIRNTRPED----AGEYQCIAVNGAGEVTSKG 2146
>SB_12405| Best HMM Match : I-set (HMM E-Value=8.8e-21)
Length = 198
Score = 27.5 bits (58), Expect = 9.8
Identities = 15/57 (26%), Positives = 23/57 (40%)
Frame = +1
Query: 388 HRWLHEAYGLGRFGYAQPERFHYSISGRQERPEDY*YHTNNLQCCGINSPADWADHG 558
HR + Y GR+ + E + ++ R RPED QC +N + G
Sbjct: 22 HRESEQIYDGGRYSIVEEENGLFVLTIRNTRPED----AGEYQCIAVNGAGEVTSKG 74
>SB_3828| Best HMM Match : Tetraspannin (HMM E-Value=6.7e-08)
Length = 542
Score = 27.5 bits (58), Expect = 9.8
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +2
Query: 305 GAVKENHCMIITFSVFLLIIFVAELAVG 388
GA K+N+ ++ +V +IFV E+++G
Sbjct: 82 GAFKQNNRVLTILAVLFFVIFVTEISLG 109
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,279,501
Number of Sequences: 59808
Number of extensions: 378283
Number of successful extensions: 1109
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1001
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1109
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1620947750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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