BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20124 (631 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 29 0.42 SPAC328.07c |||heavy metal ion homeostasis protein |Schizosaccha... 28 1.3 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 3.9 SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 25 6.8 SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharom... 25 6.8 SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce... 25 9.0 SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 25 9.0 SPBC1711.07 |||WD repeat protein Rrb1 |Schizosaccharomyces pombe... 25 9.0 >SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 29.5 bits (63), Expect = 0.42 Identities = 18/72 (25%), Positives = 32/72 (44%), Gaps = 1/72 (1%) Frame = +3 Query: 246 RLAGHPLPSMWNQRSLKTF-LQHRNSKLLLKRLCQQRVTMKATEKRPSLNWRSLSQLRLA 422 R+ G PS Q+ + L +NS++ C Q+ T++ +K NW ++ L Sbjct: 268 RIIGFDPPSWKTQQMMSHLNLSRKNSEVSKTCKCLQKETIRCNKKSNCYNWNGIAALETY 327 Query: 423 KTSGPLVIGKKK 458 T + I K+ Sbjct: 328 TTECHIQIPDKE 339 >SPAC328.07c |||heavy metal ion homeostasis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 27.9 bits (59), Expect = 1.3 Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 1/77 (1%) Frame = +2 Query: 221 TPLEPYPCQTRGTSTAIDVEPAFVENFPPAQEFEVTPKALVSATSDYESDGEEAF-PELE 397 TP+ +P + R T+ + ++ AF PP + E + A + +++ +GEEAF L Sbjct: 12 TPIPEFP-ENRSTNRS-ELAAAFE---PPDDDVEYSETAPLYSSARASIEGEEAFYQHLS 66 Query: 398 VPEPIEVSKNFWTVSDR 448 P+P S + + +DR Sbjct: 67 TPDPGNDSGHVRSSNDR 83 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 26.2 bits (55), Expect = 3.9 Identities = 17/61 (27%), Positives = 28/61 (45%) Frame = +2 Query: 179 SHGNATRRDSFGPPTPLEPYPCQTRGTSTAIDVEPAFVENFPPAQEFEVTPKALVSATSD 358 S N + FG P +P P ++ G + I V+P+ E+ P+ T + SA S Sbjct: 235 SQENKPKPTPFGSGGPSKPTPFESHGPAKQISVQPS--EHPKPSISTTTTGSSYRSAESS 292 Query: 359 Y 361 + Sbjct: 293 H 293 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 25.4 bits (53), Expect = 6.8 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 258 HPLPSMWNQRSLKTFLQHRNSKLLLKR 338 H L S NQ+ FL+ NSKL+ +R Sbjct: 36 HQLISQRNQKFQANFLEWENSKLVYRR 62 >SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharomyces pombe|chr 2|||Manual Length = 1466 Score = 25.4 bits (53), Expect = 6.8 Identities = 13/38 (34%), Positives = 17/38 (44%) Frame = +3 Query: 459 YYWTQVLWEMCPPFTKCSASAPSDAGYWGVHRPPCPNR 572 + W Q P T+C P DAG +H PCP + Sbjct: 1317 FEWRQPTGYKRTPLTECEGGVPLDAG--TLH--PCPGK 1350 >SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 25.0 bits (52), Expect = 9.0 Identities = 14/39 (35%), Positives = 18/39 (46%) Frame = +2 Query: 149 SFLREDAR*LSHGNATRRDSFGPPTPLEPYPCQTRGTST 265 SF +E+ +H N T +S GP P Y T T T Sbjct: 61 SFYKENT--FAHHNTTGSNSVGPKKPFLDYHHSTSNTPT 97 >SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizosaccharomyces pombe|chr 1|||Manual Length = 950 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = +2 Query: 395 EVPEPIEVSKNFWTVSDRKKEVLLDAGALGDVPAV 499 +V EP+ V + + +V KKEVL + L VP++ Sbjct: 388 DVIEPVMVPQWYLSVDSLKKEVLKSSNKLKLVPSL 422 >SPBC1711.07 |||WD repeat protein Rrb1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 480 Score = 25.0 bits (52), Expect = 9.0 Identities = 8/24 (33%), Positives = 15/24 (62%) Frame = -3 Query: 593 WSNFDLKPVRTRGSMYTPITGI*W 522 WS +DL+ +++ S+ TP+ W Sbjct: 362 WSVWDLRSLKSSSSVATPVASFKW 385 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,704,932 Number of Sequences: 5004 Number of extensions: 58718 Number of successful extensions: 158 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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