SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20124
         (631 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual        29   0.42 
SPAC328.07c |||heavy metal ion homeostasis protein |Schizosaccha...    28   1.3  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    26   3.9  
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc...    25   6.8  
SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharom...    25   6.8  
SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce...    25   9.0  
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos...    25   9.0  
SPBC1711.07 |||WD repeat protein Rrb1 |Schizosaccharomyces pombe...    25   9.0  

>SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 433

 Score = 29.5 bits (63), Expect = 0.42
 Identities = 18/72 (25%), Positives = 32/72 (44%), Gaps = 1/72 (1%)
 Frame = +3

Query: 246 RLAGHPLPSMWNQRSLKTF-LQHRNSKLLLKRLCQQRVTMKATEKRPSLNWRSLSQLRLA 422
           R+ G   PS   Q+ +    L  +NS++     C Q+ T++  +K    NW  ++ L   
Sbjct: 268 RIIGFDPPSWKTQQMMSHLNLSRKNSEVSKTCKCLQKETIRCNKKSNCYNWNGIAALETY 327

Query: 423 KTSGPLVIGKKK 458
            T   + I  K+
Sbjct: 328 TTECHIQIPDKE 339


>SPAC328.07c |||heavy metal ion homeostasis protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 277

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
 Frame = +2

Query: 221 TPLEPYPCQTRGTSTAIDVEPAFVENFPPAQEFEVTPKALVSATSDYESDGEEAF-PELE 397
           TP+  +P + R T+ + ++  AF    PP  + E +  A + +++    +GEEAF   L 
Sbjct: 12  TPIPEFP-ENRSTNRS-ELAAAFE---PPDDDVEYSETAPLYSSARASIEGEEAFYQHLS 66

Query: 398 VPEPIEVSKNFWTVSDR 448
            P+P   S +  + +DR
Sbjct: 67  TPDPGNDSGHVRSSNDR 83


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 17/61 (27%), Positives = 28/61 (45%)
 Frame = +2

Query: 179 SHGNATRRDSFGPPTPLEPYPCQTRGTSTAIDVEPAFVENFPPAQEFEVTPKALVSATSD 358
           S  N  +   FG   P +P P ++ G +  I V+P+  E+  P+     T  +  SA S 
Sbjct: 235 SQENKPKPTPFGSGGPSKPTPFESHGPAKQISVQPS--EHPKPSISTTTTGSSYRSAESS 292

Query: 359 Y 361
           +
Sbjct: 293 H 293


>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 143

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +3

Query: 258 HPLPSMWNQRSLKTFLQHRNSKLLLKR 338
           H L S  NQ+    FL+  NSKL+ +R
Sbjct: 36  HQLISQRNQKFQANFLEWENSKLVYRR 62


>SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1466

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/38 (34%), Positives = 17/38 (44%)
 Frame = +3

Query: 459  YYWTQVLWEMCPPFTKCSASAPSDAGYWGVHRPPCPNR 572
            + W Q       P T+C    P DAG   +H  PCP +
Sbjct: 1317 FEWRQPTGYKRTPLTECEGGVPLDAG--TLH--PCPGK 1350


>SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 14/39 (35%), Positives = 18/39 (46%)
 Frame = +2

Query: 149 SFLREDAR*LSHGNATRRDSFGPPTPLEPYPCQTRGTST 265
           SF +E+    +H N T  +S GP  P   Y   T  T T
Sbjct: 61  SFYKENT--FAHHNTTGSNSVGPKKPFLDYHHSTSNTPT 97


>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
           Vas1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 950

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 13/35 (37%), Positives = 21/35 (60%)
 Frame = +2

Query: 395 EVPEPIEVSKNFWTVSDRKKEVLLDAGALGDVPAV 499
           +V EP+ V + + +V   KKEVL  +  L  VP++
Sbjct: 388 DVIEPVMVPQWYLSVDSLKKEVLKSSNKLKLVPSL 422


>SPBC1711.07 |||WD repeat protein Rrb1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 480

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 8/24 (33%), Positives = 15/24 (62%)
 Frame = -3

Query: 593 WSNFDLKPVRTRGSMYTPITGI*W 522
           WS +DL+ +++  S+ TP+    W
Sbjct: 362 WSVWDLRSLKSSSSVATPVASFKW 385


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,704,932
Number of Sequences: 5004
Number of extensions: 58718
Number of successful extensions: 158
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -