BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20110
(575 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 28 0.85
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 2.0
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 6.0
SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 25 6.0
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 25 6.0
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 25 6.0
SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 25 7.9
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 28.3 bits (60), Expect = 0.85
Identities = 18/61 (29%), Positives = 30/61 (49%)
Frame = +1
Query: 112 YDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRWKATSIRYLFTC 291
Y+ P ++S + +L S L LD++ K L+L FP++I + R + F C
Sbjct: 153 YELPDPETKKSKIK--ALQSQLVRVNGELDDLQKHLTLSFPTLIAKSRVFFGQLMKHFYC 210
Query: 292 L 294
L
Sbjct: 211 L 211
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.1 bits (57), Expect = 2.0
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 65 PRHSTTMARHIGRITITT-PSVLTFGKACWTHI 160
P H+TT R I +I I PS+LT G +C HI
Sbjct: 553 PVHATT--RFIAQIAILELPSILTTGYSCVMHI 583
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 25.4 bits (53), Expect = 6.0
Identities = 18/51 (35%), Positives = 27/51 (52%)
Frame = +1
Query: 103 YHHYDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGR 255
+ Y F +E +T S + NL E+++L NM+ EL KFP + GR
Sbjct: 1484 FTQYRLFDVRGKERTSNTMSTY-NL-EEVEYLVNMVDELLNKFPDVNFTGR 1532
>SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 6.0
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = +1
Query: 52 AAVSAAPQYYHGSSHWPYHHYDPFSP 129
A +A PQYY +P HY P P
Sbjct: 502 ATDAAKPQYYIPKDPYPVPHYYPQQP 527
>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 851
Score = 25.4 bits (53), Expect = 6.0
Identities = 10/39 (25%), Positives = 20/39 (51%)
Frame = +1
Query: 139 ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGR 255
E + + +W+++ +MQ D ++L F I N G+
Sbjct: 104 EGSTNVNEVWNDITEDMQSQDFSTEDLKQLFLLIFNNGK 142
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 25.4 bits (53), Expect = 6.0
Identities = 20/59 (33%), Positives = 34/59 (57%), Gaps = 5/59 (8%)
Frame = +1
Query: 199 DNMMKELSLKFPSII-NEGRWKA-TSI---RYLFTCLVTNRKTST*KRKMEC*WCRLTV 360
+N++KE++ K+ SII N+ + K+ TSI YL+ V K ++K+ W +TV
Sbjct: 6 NNLLKEVAAKYASIILNDAKIKSLTSINSAEYLYDSFVEISKLPL-EKKLPLKWKLITV 63
>SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr
3|||Manual
Length = 872
Score = 25.0 bits (52), Expect = 7.9
Identities = 7/25 (28%), Positives = 16/25 (64%)
Frame = +1
Query: 103 YHHYDPFSPYVRESMLDTHSLWSNL 177
+ H+DP+S ++ ++ + H W+ L
Sbjct: 292 FFHFDPYSLFISNNLEEIHINWNIL 316
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,370,238
Number of Sequences: 5004
Number of extensions: 48017
Number of successful extensions: 135
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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