BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20110 (575 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 28 0.85 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 2.0 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 6.0 SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 25 6.0 SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 25 6.0 SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 25 6.0 SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 25 7.9 >SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizosaccharomyces pombe|chr 3|||Manual Length = 640 Score = 28.3 bits (60), Expect = 0.85 Identities = 18/61 (29%), Positives = 30/61 (49%) Frame = +1 Query: 112 YDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRWKATSIRYLFTC 291 Y+ P ++S + +L S L LD++ K L+L FP++I + R + F C Sbjct: 153 YELPDPETKKSKIK--ALQSQLVRVNGELDDLQKHLTLSFPTLIAKSRVFFGQLMKHFYC 210 Query: 292 L 294 L Sbjct: 211 L 211 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 27.1 bits (57), Expect = 2.0 Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = +2 Query: 65 PRHSTTMARHIGRITITT-PSVLTFGKACWTHI 160 P H+TT R I +I I PS+LT G +C HI Sbjct: 553 PVHATT--RFIAQIAILELPSILTTGYSCVMHI 583 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 25.4 bits (53), Expect = 6.0 Identities = 18/51 (35%), Positives = 27/51 (52%) Frame = +1 Query: 103 YHHYDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGR 255 + Y F +E +T S + NL E+++L NM+ EL KFP + GR Sbjct: 1484 FTQYRLFDVRGKERTSNTMSTY-NL-EEVEYLVNMVDELLNKFPDVNFTGR 1532 >SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 630 Score = 25.4 bits (53), Expect = 6.0 Identities = 11/26 (42%), Positives = 13/26 (50%) Frame = +1 Query: 52 AAVSAAPQYYHGSSHWPYHHYDPFSP 129 A +A PQYY +P HY P P Sbjct: 502 ATDAAKPQYYIPKDPYPVPHYYPQQP 527 >SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 851 Score = 25.4 bits (53), Expect = 6.0 Identities = 10/39 (25%), Positives = 20/39 (51%) Frame = +1 Query: 139 ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGR 255 E + + +W+++ +MQ D ++L F I N G+ Sbjct: 104 EGSTNVNEVWNDITEDMQSQDFSTEDLKQLFLLIFNNGK 142 >SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 471 Score = 25.4 bits (53), Expect = 6.0 Identities = 20/59 (33%), Positives = 34/59 (57%), Gaps = 5/59 (8%) Frame = +1 Query: 199 DNMMKELSLKFPSII-NEGRWKA-TSI---RYLFTCLVTNRKTST*KRKMEC*WCRLTV 360 +N++KE++ K+ SII N+ + K+ TSI YL+ V K ++K+ W +TV Sbjct: 6 NNLLKEVAAKYASIILNDAKIKSLTSINSAEYLYDSFVEISKLPL-EKKLPLKWKLITV 63 >SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr 3|||Manual Length = 872 Score = 25.0 bits (52), Expect = 7.9 Identities = 7/25 (28%), Positives = 16/25 (64%) Frame = +1 Query: 103 YHHYDPFSPYVRESMLDTHSLWSNL 177 + H+DP+S ++ ++ + H W+ L Sbjct: 292 FFHFDPYSLFISNNLEEIHINWNIL 316 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,370,238 Number of Sequences: 5004 Number of extensions: 48017 Number of successful extensions: 135 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 246098644 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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