BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20104 (663 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 116 2e-27 SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 106 2e-24 SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 54 1e-08 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.11 SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.4 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.4 SPBC36.09 |sap61||U2 snRNP-associated protein sap61|Schizosaccha... 27 1.8 SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.8 SPBC18H10.05 |||WD repeat protein Wdr44 family, WD repeat protei... 27 2.4 SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 3.2 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 3.2 SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 4.2 SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 26 5.6 SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 25 7.4 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 25 7.4 SPBC336.06c |rnh1||ribonuclease H Rnh1|Schizosaccharomyces pombe... 25 7.4 SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 9.7 SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 25 9.7 SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 25 9.7 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 9.7 >SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 116 bits (280), Expect = 2e-27 Identities = 55/84 (65%), Positives = 71/84 (84%) Frame = +2 Query: 8 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 187 P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66 Query: 188 YGLVVAVLIAGALQEPANYPLYKG 259 YGLVV+VLI+G L++ + LY G Sbjct: 67 YGLVVSVLISGNLKQILS--LYSG 88 Score = 116 bits (278), Expect = 4e-27 Identities = 57/64 (89%), Positives = 60/64 (93%) Frame = +1 Query: 256 GFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIV 435 GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQPRLFV MILILIFAEVLGLYGLIV Sbjct: 88 GFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQPRLFVAMILILIFAEVLGLYGLIV 147 Query: 436 AIYL 447 A+ L Sbjct: 148 ALLL 151 >SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 106 bits (255), Expect = 2e-24 Identities = 47/84 (55%), Positives = 63/84 (75%) Frame = +2 Query: 8 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 187 PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII + Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66 Query: 188 YGLVVAVLIAGALQEPANYPLYKG 259 YGLV++VLIAG + +Y L+ G Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSG 90 Score = 96.7 bits (230), Expect = 3e-21 Identities = 45/64 (70%), Positives = 54/64 (84%) Frame = +1 Query: 256 GFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIV 435 GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q R+FV M+LILIFAEVLGLYGLIV Sbjct: 90 GFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQDRIFVSMVLILIFAEVLGLYGLIV 149 Query: 436 AIYL 447 + L Sbjct: 150 GLIL 153 Score = 32.7 bits (71), Expect = 0.049 Identities = 17/74 (22%), Positives = 38/74 (51%), Gaps = 2/74 (2%) Frame = +1 Query: 232 ASQLPPLQGFIHLGAGLAVG--FSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFA 405 +S L P+ AG+ FS L AG+ + G A +P + + ++ ++ + Sbjct: 2 SSNLCPIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMS 61 Query: 406 EVLGLYGLIVAIYL 447 ++G+YGL++++ + Sbjct: 62 GIIGVYGLVMSVLI 75 >SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces pombe|chr 1|||Manual Length = 199 Score = 54.4 bits (125), Expect = 1e-08 Identities = 23/75 (30%), Positives = 45/75 (60%), Gaps = 2/75 (2%) Frame = +2 Query: 26 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 205 +G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104 Query: 206 VLIAGALQE--PANY 244 ++ + + + PA + Sbjct: 105 IVFSAKINDINPAGF 119 Score = 53.6 bits (123), Expect = 2e-08 Identities = 25/64 (39%), Positives = 35/64 (54%) Frame = +1 Query: 256 GFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIV 435 GF G+ VG L G +GI G + AQ LFV ++++ IF VLGL+GLIV Sbjct: 128 GFALFWGGITVGLCNLICGVCVGITGSSAALADAQDASLFVKVLVVEIFGSVLGLFGLIV 187 Query: 436 AIYL 447 + + Sbjct: 188 GLLI 191 Score = 40.3 bits (90), Expect = 2e-04 Identities = 20/58 (34%), Positives = 32/58 (55%) Frame = +1 Query: 268 LGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAI 441 LG V F + A + I I G + + G + PR+ ++ +IF EV+ +Y LI+AI Sbjct: 48 LGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIAI 105 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 31.5 bits (68), Expect = 0.11 Identities = 20/57 (35%), Positives = 28/57 (49%) Frame = -2 Query: 425 P*RPNTSAKIRIRIIPTNNLGC*AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 255 P RP ++A ++ PT +VP P++P MP P+A P A AP NP Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746 >SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 281 Score = 27.9 bits (59), Expect = 1.4 Identities = 17/39 (43%), Positives = 25/39 (64%), Gaps = 3/39 (7%) Frame = -1 Query: 453 CVQVDGDD-KSV--KTQYFSENKNKNHSDE*PRLLSSTT 346 C++VD +D K + K+QY +EN N N + P L S+TT Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTNSINNSP-LSSNTT 270 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.9 bits (59), Expect = 1.4 Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 2/31 (6%) Frame = -2 Query: 356 AVPRTPAS--PTMPMAKPAARPENPTAKPAP 270 A P P S P++PM PAA P P+A AP Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457 >SPBC36.09 |sap61||U2 snRNP-associated protein sap61|Schizosaccharomyces pombe|chr 2|||Manual Length = 492 Score = 27.5 bits (58), Expect = 1.8 Identities = 13/44 (29%), Positives = 25/44 (56%) Frame = +3 Query: 372 IRRNDSYSYFR*SIGSLRTYRRHLPVHKINALNTHHSRRPCSEP 503 I +D + F S+G ++ + + P HK+ L+ +S +P S+P Sbjct: 76 INADDDLTEFYKSLGEIQEFHKKYPDHKVEDLSQLYSIKP-SQP 118 >SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 27.5 bits (58), Expect = 1.8 Identities = 22/72 (30%), Positives = 32/72 (44%) Frame = +2 Query: 11 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 190 +YGPF+ A+ FS Y +G G + S+ K +I A II Y Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137 Query: 191 GLVVAVLIAGAL 226 ++AVL+ G L Sbjct: 138 TTIIAVLLWGIL 149 >SPBC18H10.05 |||WD repeat protein Wdr44 family, WD repeat protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 586 Score = 27.1 bits (57), Expect = 2.4 Identities = 12/43 (27%), Positives = 20/43 (46%) Frame = -1 Query: 660 ATRLHRSGXYVKTSREQMYXQEWKGAPKPYARWTRYRRRPSLC 532 A+ + +SG Y+ T+ + + WK P R T + P C Sbjct: 191 ASEISKSGKYLATAGKDAIIRVWKVIETPERRETLLKEGPQSC 233 >SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1729 Score = 26.6 bits (56), Expect = 3.2 Identities = 17/80 (21%), Positives = 39/80 (48%), Gaps = 3/80 (3%) Frame = +2 Query: 23 FFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKSIIPVVMAGIIAIYGL 196 F+G ++G + F+ + + ++ G S LI+ S+ P+V++ IIA + Sbjct: 1439 FYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMSVAPLVLSAIIAFFFF 1498 Query: 197 VVAVLIAGALQEPA-NYPLY 253 + +++ L + + Y +Y Sbjct: 1499 CLGIMLRPILGDRSKTYGVY 1518 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 26.6 bits (56), Expect = 3.2 Identities = 16/52 (30%), Positives = 21/52 (40%) Frame = -2 Query: 356 AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNPCKGGSWLAPGGHQQSGQP 201 +VP+ PA P +P A P P P + P GG A Q + P Sbjct: 678 SVPQPPAVPVVPEAGQLNEPVVPPLPPHDETQEPQVGGDVKATEHTQPTKTP 729 Score = 25.4 bits (53), Expect = 7.4 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%) Frame = -2 Query: 356 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 267 +VP+ PA+P +P A +P A P P AP+ Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546 >SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 422 Score = 26.2 bits (55), Expect = 4.2 Identities = 16/52 (30%), Positives = 21/52 (40%), Gaps = 3/52 (5%) Frame = -2 Query: 344 TPASPTMPMAKPAARPENPTAKPAPK---WMNPCKGGSWLAPGGHQQSGQPR 198 TPAS T+P++ P PAP NP G + + SG R Sbjct: 297 TPASATVPISPARGFPTTSAYNPAPPAYGMANPAYGSTGIRSSSIPSSGSIR 348 >SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 2609 Score = 25.8 bits (54), Expect = 5.6 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = -1 Query: 558 RYRRRPSLCASSDSDNDIEARSTG 487 R R P L SS+ D+ +EARS+G Sbjct: 1640 RKRLMPCLLQSSELDSKLEARSSG 1663 >SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 288 Score = 25.4 bits (53), Expect = 7.4 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = +2 Query: 152 IIPVVMAGIIAIYGLVVAVLIAGAL 226 II +AG+IA + +++ IAG + Sbjct: 193 IITATIAGVIAAFSVIITATIAGVI 217 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 25.4 bits (53), Expect = 7.4 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -1 Query: 528 SSDSDNDIEARSTGDGSGVCSGRLFCVQVDGDD 430 SSD+D D + G G+ + + L VDG+D Sbjct: 186 SSDNDEDPNDTTVGIGAALAASILSVDSVDGED 218 >SPBC336.06c |rnh1||ribonuclease H Rnh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 264 Score = 25.4 bits (53), Expect = 7.4 Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 1/70 (1%) Frame = -1 Query: 642 SGXYVKTSREQMYXQEWKGAPKPYARWT-RYRRRPSLCASSDSDNDIEARSTGDGSGVCS 466 SG Y +++ Y + AR + +YR++ S S++ D +I + T + S CS Sbjct: 62 SGPYRRSTTSYGYSPYSSSSSNYSARHSDKYRKKISRSYSTEKDIEIFSNDTHEKSIACS 121 Query: 465 GRLFCVQVDG 436 R V DG Sbjct: 122 DRQ-VVYADG 130 >SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1405 Score = 25.0 bits (52), Expect = 9.7 Identities = 8/19 (42%), Positives = 13/19 (68%) Frame = -3 Query: 331 PRCLWRNRQPDQRILQPNQ 275 PRCLW +Q +++PN+ Sbjct: 582 PRCLWTGKQVFTVLMKPNR 600 >SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein intermediate chain Dic1|Schizosaccharomyces pombe|chr 2|||Manual Length = 544 Score = 25.0 bits (52), Expect = 9.7 Identities = 10/31 (32%), Positives = 14/31 (45%) Frame = +1 Query: 184 HLRSGRGCPDCWCPPGASQLPPLQGFIHLGA 276 HL+ P C P A ++ L F+H A Sbjct: 112 HLKHSTKAPKCSLPTSALEIDHLNNFLHSSA 142 >SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 579 Score = 25.0 bits (52), Expect = 9.7 Identities = 21/58 (36%), Positives = 28/58 (48%), Gaps = 2/58 (3%) Frame = +1 Query: 250 LQGFIHLGAGLAVGFSGLAAGFAIGIVGD-AGVRGTAQQPRLFVGMIL-ILIFAEVLG 417 L G + +A+G +GLA G IG+ G A A LF G+ L +I A LG Sbjct: 163 LSGTRKMRRRIAMGLAGLAGGALIGLTGGLAAPFVAAGLGTLFAGLGLGTMIGATYLG 220 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 25.0 bits (52), Expect = 9.7 Identities = 10/27 (37%), Positives = 14/27 (51%) Frame = +2 Query: 410 YWVFTDLSSPSTCTQNKRPEHTPLPSP 490 YW +T +SP+ +N R P P P Sbjct: 497 YWYYTVFNSPTIIEKNFRQSVGPKPIP 523 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,891,993 Number of Sequences: 5004 Number of extensions: 61960 Number of successful extensions: 211 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 193 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 211 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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