Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20092
(738 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 79 4e-15
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 75 4e-14
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 74 9e-14
At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical t... 48 9e-06
At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 47 1e-05
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 41 7e-04
At1g72190.1 68414.m08347 oxidoreductase family protein similar t... 40 0.002
At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containi... 35 0.065
At5g62220.1 68418.m07813 exostosin family protein contains Pfam ... 31 0.80
At5g28310.1 68418.m03437 oxidoreductase-related low similarity t... 30 1.8
At2g45630.2 68415.m05674 oxidoreductase family protein low simil... 30 1.8
At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) simil... 30 1.8
At1g06460.1 68414.m00684 31.2 kDa small heat shock family protei... 30 1.8
At3g28790.1 68416.m03593 expressed protein 29 4.3
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 29 4.3
At4g15810.1 68417.m02406 chloroplast outer membrane protein, put... 28 7.4
At3g26420.1 68416.m03295 glycine-rich RNA-binding protein simila... 28 7.4
At4g11420.1 68417.m01840 eukaryotic translation initiation facto... 27 9.8
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 78.6 bits (185), Expect = 4e-15
Identities = 41/106 (38%), Positives = 64/106 (60%)
Frame = +2
Query: 164 QDLQGRTSYGDTQPRRSGCAFSNSSDQRGVDAGVKLKVVGRAGAGVDNIDVDSAGKKGVG 343
+DL+ + + D RSG + + A +LKVVGRAG G+DN+D+ +A + G
Sbjct: 114 EDLKKKVAESDALIVRSGTKVTREVFEA---AKGRLKVVGRAGVGIDNVDLQAATEHGCL 170
Query: 344 VINAPGANALSACELTCTLMLVLARHVVPASNALKAGRWDRALYTG 481
V+NAP AN ++A E L+ +AR+V A ++KAG+W+R+ Y G
Sbjct: 171 VVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGKWERSKYVG 216
Score = 37.5 bits (83), Expect = 0.009
Identities = 20/59 (33%), Positives = 33/59 (55%)
Frame = +3
Query: 87 VLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKEVLTQA 263
+L+ + +G LL +G + +S E+L ++ DAL+VRS T+VT+EV A
Sbjct: 85 ILVTEKLGEAGVNLLREFGDVDCSY-DLSPEDLKKKVAESDALIVRSGTKVTREVFEAA 142
Score = 37.5 bits (83), Expect = 0.009
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +1
Query: 508 LLGLGRVGREVATRMYAFGMNIIGFDPFVSADQ 606
++G G+VG EVA R GM +I DP+ AD+
Sbjct: 226 VMGFGKVGTEVARRAKGLGMTVISHDPYAPADR 258
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 75.4 bits (177), Expect = 4e-14
Identities = 35/72 (48%), Positives = 48/72 (66%)
Frame = +2
Query: 266 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASNAL 445
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E LM +AR+V A ++
Sbjct: 124 RLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASV 183
Query: 446 KAGRWDRALYTG 481
KAG W R Y G
Sbjct: 184 KAGEWKRNKYVG 195
Score = 38.3 bits (85), Expect = 0.005
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = +1
Query: 508 LLGLGRVGREVATRMYAFGMNIIGFDPFVSADQ 606
+LG G+VG EVA R GM +I DP+ AD+
Sbjct: 205 VLGFGKVGTEVARRAKGLGMRVIAHDPYAPADR 237
Score = 28.3 bits (60), Expect = 5.6
Identities = 14/28 (50%), Positives = 21/28 (75%)
Frame = +3
Query: 168 ISKEELLMEIPNHDALVVRSATQVTKEV 251
++ EEL ++I DAL+VRS T+V +EV
Sbjct: 90 MTPEELNIKISLCDALIVRSGTKVGREV 117
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 74.1 bits (174), Expect = 9e-14
Identities = 33/72 (45%), Positives = 49/72 (68%)
Frame = +2
Query: 266 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASNAL 445
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E L+ +AR++ A ++
Sbjct: 109 RLKVVGRAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASI 168
Query: 446 KAGRWDRALYTG 481
KAG+W R Y G
Sbjct: 169 KAGKWTRNKYVG 180
Score = 37.9 bits (84), Expect = 0.007
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +1
Query: 508 LLGLGRVGREVATRMYAFGMNIIGFDPFVSADQ 606
+LG G+VG EVA R GM++I DP+ AD+
Sbjct: 190 VLGFGKVGSEVARRARGLGMHVITHDPYAPADR 222
Score = 32.3 bits (70), Expect = 0.35
Identities = 19/56 (33%), Positives = 32/56 (57%)
Frame = +3
Query: 84 SVLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKEV 251
++L+ + +G +LL Y + +S EEL +I DAL+VRS T+V ++V
Sbjct: 48 TILVTEKLGQAGIDLLKKYANVDCSY-DLSLEELCTKISLCDALIVRSGTKVGRDV 102
>At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical to
GI:7677266
Length = 384
Score = 47.6 bits (108), Expect = 9e-06
Identities = 24/67 (35%), Positives = 37/67 (55%)
Frame = +2
Query: 269 LKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASNALK 448
LK++ AG G D+ID+ +A G+ V G+N +S E +L+L R+ VP N +
Sbjct: 120 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 179
Query: 449 AGRWDRA 469
G W+ A
Sbjct: 180 KGEWNVA 186
>At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent
hydroxypyruvate reductase identical to hydroxypyruvate
reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant
Cell Physiol 1997 Apr;38(4):449-55)
Length = 386
Score = 46.8 bits (106), Expect = 1e-05
Identities = 21/64 (32%), Positives = 33/64 (51%)
Frame = +2
Query: 272 KVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASNALKA 451
K G +N+DV++A K G+ V N PG + EL +L L AR +V A ++
Sbjct: 88 KAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRG 147
Query: 452 GRWD 463
G ++
Sbjct: 148 GLYE 151
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 41.1 bits (92), Expect = 7e-04
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +2
Query: 269 LKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASNALK 448
L++V G+D ID+ +KG+ V N P +L L+L L R + ++
Sbjct: 68 LEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVR 127
Query: 449 AGRW 460
+G+W
Sbjct: 128 SGKW 131
Score = 30.3 bits (65), Expect = 1.4
Identities = 20/77 (25%), Positives = 39/77 (50%), Gaps = 1/77 (1%)
Frame = +1
Query: 508 LLGLGRVGREVATRMYAFGMNIIGFDPFVSADQCAQFHCTKMEL-EDIWL*RTTSPCTLR 684
++GLGR+G +A R AF I + + D +++ T ++L ++ + P T
Sbjct: 149 IIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTVVDLAQNSDILVVACPLT-- 206
Query: 685 FIESTRNFINADVFEAV 735
E TR+ ++ V +A+
Sbjct: 207 --EQTRHIVDRQVMDAL 221
>At1g72190.1 68414.m08347 oxidoreductase family protein similar to
D-3-phosphoglycerate dehydrogenase from Arabidopsis
thaliana [SP|O04130], glyoxylate reductase from Homo
sapiens (gi:6002730); contains Pfam D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain PF02826
Length = 373
Score = 39.5 bits (88), Expect = 0.002
Identities = 21/69 (30%), Positives = 34/69 (49%), Gaps = 3/69 (4%)
Frame = +2
Query: 221 AFSNSSDQRGVDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPG---ANALSACELT 391
A + D + +K++ + G G+D +D+D+A K G+ V P NA S E+
Sbjct: 100 AMTMQMDSNVISRASNIKLIMQYGVGLDGVDIDAATKHGIKVARIPSEGTGNAASCSEMA 159
Query: 392 CTLMLVLAR 418
LML L +
Sbjct: 160 IYLMLGLLK 168
>At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containing
protein ESTs gb|F20110 and gb|F20109 come from this gene;
contains Pfam profile PF00515: TPR Domain
Length = 1558
Score = 34.7 bits (76), Expect = 0.065
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +2
Query: 86 GFDRRRGWRQVCRTPKR-LRNRHHHQGQDLQGRTSYGDTQPRRSGCAFSNSSDQRGV 253
G R+ RQ KR L N+HH++ QD+Q + Y Q G + S SS +R +
Sbjct: 1165 GAGRKSRQRQPDLMKKRMLLNKHHNRNQDVQQQNIYSPLQKTSKGPSLSKSSPRRAL 1221
>At5g62220.1 68418.m07813 exostosin family protein contains Pfam
profile: PF03016 Exostosin family
Length = 517
Score = 31.1 bits (67), Expect = 0.80
Identities = 21/69 (30%), Positives = 36/69 (52%)
Frame = -3
Query: 268 LHACVNTSLVT*VAERTTRASWLGISIRSSSLEILALVVVAIP*AFRSSAHLAPTPSTIK 89
LH+ N S +A + +WL +S+ S L IL L + +IP R P+P+ +
Sbjct: 35 LHSVGNRSSY--IAASRSHCTWLILSLLSLQL-ILFLTLRSIPFPHRHIPENFPSPAAVV 91
Query: 88 TDLMSTTIL 62
T ++TT++
Sbjct: 92 TTTVTTTVI 100
>At5g28310.1 68418.m03437 oxidoreductase-related low similarity to
glyoxylate reductase from Thermococcus litoralis
[gi:13515409]
Length = 233
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = +1
Query: 508 LLGLGRVGREVATRMYAFGMNI 573
++GLG +G +VATR+ AFG I
Sbjct: 119 IVGLGSIGSKVATRLKAFGCQI 140
>At2g45630.2 68415.m05674 oxidoreductase family protein low
similarity to SP|P36234 Glycerate dehydrogenase (EC
1.1.1.29) (NADH-dependent hydroxypyruvate reductase)
{Hyphomicrobium methylovorum}; contains Pfam profile
PF00389: D-isomer specific 2-hydroxyacid dehydrogenase,
catalytic domain
Length = 338
Score = 29.9 bits (64), Expect = 1.8
Identities = 22/76 (28%), Positives = 34/76 (44%)
Frame = +1
Query: 508 LLGLGRVGREVATRMYAFGMNIIGFDPFVSADQCAQFHCTKMELEDIWL*RTTSPCTLRF 687
++GLG +G +VATR+ AFG I + +H M++E++
Sbjct: 173 IVGLGSIGSKVATRLDAFGCQ-ISYSSRNRKPYDVPYH-YYMDIEEMAANSDALIICCEL 230
Query: 688 IESTRNFINADVFEAV 735
E T IN DV A+
Sbjct: 231 NEKTLRLINKDVLSAL 246
>At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) similar
to glutamate decarboxylase (gad) GI:294111 from [Petunia
hybrida]
Length = 494
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = +3
Query: 63 KMVVDIKSVLI-VDGVGAKCAELLNAYGIATTTKAKISKEELLMEI 197
++V DI VL +D + +K ++ + GIA K K ++E+LME+
Sbjct: 431 RLVADISKVLHELDTLPSKISKKMGIEGIAENVKEKKMEKEILMEV 476
>At1g06460.1 68414.m00684 31.2 kDa small heat shock family protein /
hsp20 family protein contains Pfam profile: PF00011
Hsp20/alpha crystallin family
Length = 285
Score = 29.9 bits (64), Expect = 1.8
Identities = 17/57 (29%), Positives = 25/57 (43%)
Frame = -1
Query: 516 AKEARVLPASSLPVYRARSHLPAFSALEAGTTWRARTNINVQVSSHALSALAPGALI 346
AKE P+ S + + R L GT W R+N+ S+ ++ PGA I
Sbjct: 152 AKEDIFFPSLSPNLQKERPKLDLPKLANLGTVWSPRSNVAESTHSYVVAIELPGASI 208
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/29 (44%), Positives = 15/29 (51%)
Frame = -1
Query: 372 SALAPGALITPTPFLPAESTSMLSTPAPA 286
S +P TPTP P ST STP P+
Sbjct: 271 SGASPSGSPTPTPSTPTPSTPTPSTPTPS 299
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = -1
Query: 345 TPTPFLPAESTSMLSTPAPA 286
TPTP P ST STPAP+
Sbjct: 295 TPTPSTPTPSTPTPSTPAPS 314
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 28.7 bits (61), Expect = 4.3
Identities = 24/71 (33%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = +2
Query: 2 PDARRRRTPASSLTTIALPNQDGRGH*VGFDRRRGWRQVCRTPKRLRNRHHH-QGQDLQG 178
PD RRRR+P+SS + + H R R ++ R+P RL Q L
Sbjct: 603 PDRRRRRSPSSSRSP-SRSRSPPVLHRSPSPRGRKHQRERRSPGRLSEEQDRVQNSKLLK 661
Query: 179 RTSYGDTQPRR 211
RTS DT R+
Sbjct: 662 RTSVPDTDKRK 672
>At4g15810.1 68417.m02406 chloroplast outer membrane protein,
putative similar to chloroplast protein import component
Toc159 [Pisum sativum] GI:8489806, chloroplast outer
envelope protein 86 [Pisum sativum] GI:599958,
GTP-binding protein [Pisum sativum] GI:576509
Length = 898
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +2
Query: 149 HHHQGQDLQGRTSYGDTQPRRSGCAFSNSSDQRGVDAGVKL 271
H+H +L GDT + +G +F NSS+ + V+L
Sbjct: 491 HNHTFMELDEYEGTGDTSEKLTGSSFQNSSELLSSNHSVEL 531
>At3g26420.1 68416.m03295 glycine-rich RNA-binding protein similar
to RNA-binding protein (RZ-1) GB:BAA12064 [Nicotiana
sylvestris]; contains Pfam profile: PF00076 RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 245
Score = 27.9 bits (59), Expect = 7.4
Identities = 22/60 (36%), Positives = 24/60 (40%), Gaps = 1/60 (1%)
Frame = +2
Query: 161 GQDLQGRTSYGD-TQPRRSGCAFSNSSDQRGVDAGVKLKVVGRAGAGVDNIDVDSAGKKG 337
G DL GRT D QP + G N D RG D G + R G D GK G
Sbjct: 70 GMDLDGRTITVDKAQPHQGGAGRDNDGD-RGRDRGYD-RDRSRPSGGRGGGDCFKCGKPG 127
>At4g11420.1 68417.m01840 eukaryotic translation initiation factor 3
subunit 10 / eIF-3 theta / eIF3a (TIF3A1) identical to
eukaryotic translation initiation factor 3 subunit 10
(eIF-3 theta) (Eukaryotic translation initiation factor 3
large subunit) (eIF3a) (p114). [Arabidopsis thaliana]
SWISS-PROT:Q9LD55
Length = 987
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = -2
Query: 464 GPTCQPSAHWKLGPRGEPEPTSTCRSARTR 375
GP + W GPRG + ST S+R R
Sbjct: 954 GPPAEGGDRWGSGPRGSDDRRSTFGSSRPR 983
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,138,683
Number of Sequences: 28952
Number of extensions: 295800
Number of successful extensions: 919
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 876
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 918
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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