BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20090 (550 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 134 8e-33 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 134 8e-33 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 81 8e-17 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 69 3e-13 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 43 3e-05 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 34 0.012 SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 29 0.45 SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual 29 0.45 SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ... 28 1.0 SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces po... 27 2.4 SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc... 26 4.2 SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 26 4.2 SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 5.6 SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase |S... 25 5.6 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 25 5.6 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 9.7 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 25 9.7 SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyce... 25 9.7 SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual 25 9.7 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 134 bits (324), Expect = 8e-33 Identities = 63/85 (74%), Positives = 73/85 (85%) Frame = +3 Query: 3 RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182 RI GPN+ PGKK+DL+ K IQRT+LMMG +E IED P+GNI GLVGVDQFLVK+GT+TT Sbjct: 412 RIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTT 471 Query: 183 FKNAHNMKVMKFSVSPVVRVAVEPK 257 + AHNMKVMKFSVSPVV+VAVE K Sbjct: 472 SEVAHNMKVMKFSVSPVVQVAVEVK 496 Score = 132 bits (320), Expect = 2e-32 Identities = 64/85 (75%), Positives = 70/85 (82%) Frame = +2 Query: 254 QNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIK 433 +N DLPKLVEGLKRL+KSDP V C ESGEHIVAGAGELHLEICLKDL+EDHA IP+K Sbjct: 496 KNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLK 555 Query: 434 KSDPVVSYRETVAEESDQLXLSKSP 508 S PVVSYRE+V+E S LSKSP Sbjct: 556 ISPPVVSYRESVSEPSSMTALSKSP 580 Score = 27.1 bits (57), Expect = 1.8 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +1 Query: 499 KVAHKHNRLFMKAQPM 546 K +KHNR+FM A+PM Sbjct: 578 KSPNKHNRIFMTAEPM 593 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 134 bits (324), Expect = 8e-33 Identities = 63/85 (74%), Positives = 73/85 (85%) Frame = +3 Query: 3 RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182 RI GPN+ PGKK+DL+ K IQRT+LMMG +E IED P+GNI GLVGVDQFLVK+GT+TT Sbjct: 412 RIQGPNYVPGKKDDLFIKAIQRTVLMMGSRIEPIEDCPAGNIIGLVGVDQFLVKSGTLTT 471 Query: 183 FKNAHNMKVMKFSVSPVVRVAVEPK 257 + AHNMKVMKFSVSPVV+VAVE K Sbjct: 472 SEVAHNMKVMKFSVSPVVQVAVEVK 496 Score = 132 bits (320), Expect = 2e-32 Identities = 64/85 (75%), Positives = 70/85 (82%) Frame = +2 Query: 254 QNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIK 433 +N DLPKLVEGLKRL+KSDP V C ESGEHIVAGAGELHLEICLKDL+EDHA IP+K Sbjct: 496 KNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLK 555 Query: 434 KSDPVVSYRETVAEESDQLXLSKSP 508 S PVVSYRE+V+E S LSKSP Sbjct: 556 ISPPVVSYRESVSEPSSMTALSKSP 580 Score = 27.1 bits (57), Expect = 1.8 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +1 Query: 499 KVAHKHNRLFMKAQPM 546 K +KHNR+FM A+PM Sbjct: 578 KSPNKHNRIFMTAEPM 593 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 81.4 bits (192), Expect = 8e-17 Identities = 41/81 (50%), Positives = 52/81 (64%) Frame = +2 Query: 260 PADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKS 439 P ++ KLV GL L ++DP VQ EE+GEH++ AGE+HLE CLKDL E A I I+ S Sbjct: 573 PFEMNKLVTGLDMLNQADPCVQIAVEENGEHVIMCAGEIHLERCLKDLRERFAKIEIQAS 632 Query: 440 DPVVSYRETVAEESDQLXLSK 502 P+V YRET D L +K Sbjct: 633 QPLVPYRETTIATPDLLAKNK 653 Score = 54.4 bits (125), Expect = 1e-08 Identities = 27/84 (32%), Positives = 48/84 (57%), Gaps = 1/84 (1%) Frame = +3 Query: 6 IMGPNFTPGKKEDLYEK-TIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182 + GP + P E K T++ LMMG+ + +E VP+GN+ + G+ +++T T+ + Sbjct: 487 VYGPKYDPVNPEKHITKVTVESLYLMMGQELVYLETVPAGNVFAIGGLAGTVLRTATLCS 546 Query: 183 FKNAHNMKVMKFSVSPVVRVAVEP 254 N N+ + + P+VRVA+EP Sbjct: 547 SPNGPNLVGVTQQMEPIVRVALEP 570 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 69.3 bits (162), Expect = 3e-13 Identities = 34/84 (40%), Positives = 52/84 (61%) Frame = +2 Query: 257 NPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKK 436 NP++LPKL++GL++ KS P+ EESGEH + G GE++++ L DL ++ I I+ Sbjct: 609 NPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLRTLYSEIEIRV 668 Query: 437 SDPVVSYRETVAEESDQLXLSKSP 508 SDPV + ET + S S +P Sbjct: 669 SDPVARFCETAVDTSSIKCFSDTP 692 Score = 32.7 bits (71), Expect = 0.037 Identities = 22/87 (25%), Positives = 41/87 (47%), Gaps = 3/87 (3%) Frame = +3 Query: 3 RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182 +++G N++ +ED+ I + RY ++ +G + L GVD + KT TI + Sbjct: 521 KVLGENYSLEDEEDMVVAHIAEICVPCARYRLHVDGAVAGMLVLLGGVDNSISKTATIVS 580 Query: 183 FK---NAHNMKVMKFSVSPVVRVAVEP 254 + + + + V +VAVEP Sbjct: 581 DNLKDDPYIFRPIAHMSESVFKVAVEP 607 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 43.2 bits (97), Expect = 3e-05 Identities = 21/72 (29%), Positives = 42/72 (58%), Gaps = 1/72 (1%) Frame = +2 Query: 266 DLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSD 442 D + L R + DP + ++ ES E I++G GELHLE+ ++ + ++ + + Sbjct: 486 DTTSFSKALNRFQREDPTFRVQLDNESKETIISGMGELHLEVYVERMRREYK-VDCETGK 544 Query: 443 PVVSYRETVAEE 478 P V++RET++++ Sbjct: 545 PRVAFRETLSKK 556 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 34.3 bits (75), Expect = 0.012 Identities = 20/73 (27%), Positives = 36/73 (49%), Gaps = 1/73 (1%) Frame = +2 Query: 266 DLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSD 442 D P L+E L + + DP + + E+G+ ++ G G +HL++ + L + Sbjct: 456 DEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERLVSEFGA-RASLGK 514 Query: 443 PVVSYRETVAEES 481 V YRET+ + S Sbjct: 515 VQVGYRETLIDVS 527 >SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit Tfg3|Schizosaccharomyces pombe|chr 1|||Manual Length = 241 Score = 29.1 bits (62), Expect = 0.45 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 6/49 (12%) Frame = +2 Query: 260 PADLPKLVEGLKRLAKSD--PMVQCINEESGEHIVA----GAGELHLEI 388 P D+ KL EGL++L + D +VQ +NE + GE H+++ Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDL 220 >SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual Length = 973 Score = 29.1 bits (62), Expect = 0.45 Identities = 19/56 (33%), Positives = 27/56 (48%) Frame = -2 Query: 477 SSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPATMCSPDSSLIHCTMGS 310 S T YDTTG+D ++ SS R WS+ P +P ++ I+ T GS Sbjct: 117 SDYTSVYYDTTGTDDVVYEYHPSSERHKSTNDTWSTNLPT---NPTTTAIYSTSGS 169 >SPAC21E11.06 |tif224||translation initiation factor eIF2B delta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 27.9 bits (59), Expect = 1.0 Identities = 12/33 (36%), Positives = 21/33 (63%), Gaps = 2/33 (6%) Frame = +3 Query: 138 VGVDQFLVKTGTITTFKNAHNMKV--MKFSVSP 230 +GVD F K G + +K+ N+K+ +K+ V+P Sbjct: 404 MGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTP 436 >SPACUNK4.08 |||dipeptidyl aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 793 Score = 26.6 bits (56), Expect = 2.4 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +3 Query: 147 DQFLVKTGTITTFKNAHNMKVMKFSVSPVV 236 D FLVK G + ++ +N+ FSVS +V Sbjct: 82 DTFLVKYGDLINIQDPYNLNKTLFSVSDLV 111 >SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 451 Score = 25.8 bits (54), Expect = 4.2 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%) Frame = +3 Query: 12 GPNFTP-GKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVG 143 G +F P GK ++ + Q + + RY+ DVP+G+I G+ G Sbjct: 115 GSDFDPKGKSDNEIRRFSQAFMRQLFRYIGPQTDVPAGDI-GVTG 158 >SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 25.8 bits (54), Expect = 4.2 Identities = 13/41 (31%), Positives = 22/41 (53%) Frame = +2 Query: 383 EICLKDLEEDHACIPIKKSDPVVSYRETVAEESDQLXLSKS 505 E+C +LE+D I +S +S + A++ DQL S + Sbjct: 64 ELCNFELEKDSLESMIHESSTALSAQSNTAQDGDQLASSST 104 >SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.4 bits (53), Expect = 5.6 Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 2/33 (6%) Frame = -2 Query: 375 SSPAPATMCSPDSSL--IHCTMGSDLARRLRPS 283 S+ +P+T+CSPD+++ ++ G L ++ P+ Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435 >SPCC1235.15 |dga1|SPCC548.01|diacylglycerol O-acyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 345 Score = 25.4 bits (53), Expect = 5.6 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 214 NSVYHQSCVSLLSPKP 261 NSV +SCVS+LS KP Sbjct: 172 NSVSKKSCVSILSRKP 187 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 25.4 bits (53), Expect = 5.6 Identities = 15/52 (28%), Positives = 26/52 (50%) Frame = +2 Query: 194 PQHEGDEIQCITSRACRC*AQNPADLPKLVEGLKRLAKSDPMVQCINEESGE 349 P+ EGD I + + + DL K +R K+ PM++ +++ SGE Sbjct: 248 PEPEGDRITNVMKLLIPEDSNDSLDLAK-----RRSCKTPPMIEFVSDSSGE 294 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 24.6 bits (51), Expect = 9.7 Identities = 15/56 (26%), Positives = 26/56 (46%), Gaps = 2/56 (3%) Frame = +3 Query: 90 YVEAIEDVPSGNIC--GLVGVDQFLVKTGTITTFKNAHNMKVMKFSVSPVVRVAVE 251 Y+ A ++ SG G V + L T ++ A + + K + SP+VR+ E Sbjct: 414 YIAAFSEILSGRNYDFGEVSIQLMLSCTTCVSNMMEALPLCMAKIAYSPIVRILCE 469 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 24.6 bits (51), Expect = 9.7 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = -3 Query: 116 GHILNSFNITSHHKDCTLDSLLIQ 45 G+++N F + SHH L L+ Q Sbjct: 573 GNVINGFIVRSHHDQLILKELMRQ 596 >SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 351 Score = 24.6 bits (51), Expect = 9.7 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +2 Query: 317 MVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIK 433 M + IN +S + +E CL L+E H +P++ Sbjct: 1 MKEMINLDSSSEPSIVTKQFSVEECLSKLKEQHCYVPMR 39 >SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual Length = 533 Score = 24.6 bits (51), Expect = 9.7 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +2 Query: 293 KRLAKSDPMVQCINEESGEHIVAGAGEL 376 KR A + +V C+NE E +A A EL Sbjct: 83 KRAAIAQQLVNCVNELFYEEALARAAEL 110 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,323,055 Number of Sequences: 5004 Number of extensions: 46732 Number of successful extensions: 154 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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