Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20090
(550 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 122 2e-28
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 87 7e-18
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 87 7e-18
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 87 7e-18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 79 2e-15
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 5e-06
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 45 4e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 45 4e-05
At5g05160.1 68418.m00549 leucine-rich repeat transmembrane prote... 29 1.5
At1g04650.1 68414.m00462 hypothetical protein 29 2.0
At5g59740.1 68418.m07488 UDP-galactose/UDP-glucose transporter-r... 29 2.7
At5g17300.1 68418.m02026 myb family transcription factor similar... 28 3.6
At3g46180.1 68416.m04997 UDP-galactose/UDP-glucose transporter-r... 28 3.6
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 3.6
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 4.7
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 28 4.7
At3g59130.1 68416.m06592 DC1 domain-containing protein contains ... 27 6.2
At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subu... 27 6.2
At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family pr... 27 8.3
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 122 bits (294), Expect = 2e-28
Identities = 61/83 (73%), Positives = 68/83 (81%), Gaps = 1/83 (1%)
Frame = +2
Query: 263 ADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDH-ACIPIKKS 439
+DLPKLVEGLKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL++D I KS
Sbjct: 499 SDLPKLVEGLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKS 558
Query: 440 DPVVSYRETVAEESDQLXLSKSP 508
DPVVS+RETV + S + +SKSP
Sbjct: 559 DPVVSFRETVCDRSTRTVMSKSP 581
Score = 109 bits (263), Expect = 9e-25
Identities = 50/87 (57%), Positives = 67/87 (77%), Gaps = 2/87 (2%)
Frame = +3
Query: 3 RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
RIMGPN+ PG+K+DLY K++QRT++ MG+ E +EDVP GN +VG+DQF+ K T+T
Sbjct: 410 RIMGPNYIPGEKKDLYTKSVQRTVIWMGKRQETVEDVPCGNTVAMVGLDQFITKNATLTN 469
Query: 183 FK--NAHNMKVMKFSVSPVVRVAVEPK 257
K +AH ++ MKFSVSPVVRVAV+ K
Sbjct: 470 EKEVDAHPIRAMKFSVSPVVRVAVQCK 496
Score = 27.1 bits (57), Expect = 8.3
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = +1
Query: 499 KVAHKHNRLFMKAQPM 546
K +KHNRL+M+A+PM
Sbjct: 579 KSPNKHNRLYMEARPM 594
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 87.0 bits (206), Expect = 7e-18
Identities = 39/84 (46%), Positives = 60/84 (71%)
Frame = +2
Query: 257 NPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKK 436
NP++LPK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K
Sbjct: 596 NPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKV 655
Query: 437 SDPVVSYRETVAEESDQLXLSKSP 508
+DPVVS+ ETV E S +++P
Sbjct: 656 ADPVVSFCETVVESSSMKCFAETP 679
Score = 49.6 bits (113), Expect = 1e-06
Identities = 24/87 (27%), Positives = 46/87 (52%), Gaps = 3/87 (3%)
Frame = +3
Query: 3 RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
R++G ++P +ED+ K + + + RY + P G+ + GVD ++KT T+
Sbjct: 508 RVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCN 567
Query: 183 F---KNAHNMKVMKFSVSPVVRVAVEP 254
++ + + +KF+ PVV+ A EP
Sbjct: 568 ASYDEDVYIFRALKFNTLPVVKTATEP 594
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 87.0 bits (206), Expect = 7e-18
Identities = 39/84 (46%), Positives = 60/84 (71%)
Frame = +2
Query: 257 NPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKK 436
NP++LPK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K
Sbjct: 610 NPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKV 669
Query: 437 SDPVVSYRETVAEESDQLXLSKSP 508
+DPVVS+ ETV E S +++P
Sbjct: 670 ADPVVSFCETVVESSSMKCFAETP 693
Score = 48.0 bits (109), Expect = 4e-06
Identities = 23/87 (26%), Positives = 46/87 (52%), Gaps = 3/87 (3%)
Frame = +3
Query: 3 RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
R++G ++P +ED+ K + + + RY + P G+ + GVD ++KT T+
Sbjct: 522 RVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCN 581
Query: 183 F---KNAHNMKVMKFSVSPVVRVAVEP 254
++ + + ++F+ PVV+ A EP
Sbjct: 582 ASYDEDVYIFRALQFNTLPVVKTATEP 608
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 87.0 bits (206), Expect = 7e-18
Identities = 39/84 (46%), Positives = 60/84 (71%)
Frame = +2
Query: 257 NPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKK 436
NP++LPK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K
Sbjct: 610 NPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKV 669
Query: 437 SDPVVSYRETVAEESDQLXLSKSP 508
+DPVVS+ ETV E S +++P
Sbjct: 670 ADPVVSFCETVVESSSMKCFAETP 693
Score = 48.0 bits (109), Expect = 4e-06
Identities = 23/87 (26%), Positives = 46/87 (52%), Gaps = 3/87 (3%)
Frame = +3
Query: 3 RIMGPNFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITT 182
R++G ++P +ED+ K + + + RY + P G+ + GVD ++KT T+
Sbjct: 522 RVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCN 581
Query: 183 F---KNAHNMKVMKFSVSPVVRVAVEP 254
++ + + ++F+ PVV+ A EP
Sbjct: 582 ASYDEDVYIFRALQFNTLPVVKTATEP 608
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 79.0 bits (186), Expect = 2e-15
Identities = 35/78 (44%), Positives = 54/78 (69%)
Frame = +2
Query: 257 NPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKK 436
+PAD+ L++GL+ L ++DP V+ GEH++A AGE+HLE C+KDL+E A + ++
Sbjct: 547 DPADMSALMKGLRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLKERFAKVNLEV 606
Query: 437 SDPVVSYRETVAEESDQL 490
S P+VSYRET+ + L
Sbjct: 607 SPPLVSYRETIEGDGSNL 624
Score = 52.8 bits (121), Expect = 1e-07
Identities = 22/70 (31%), Positives = 43/70 (61%)
Frame = +3
Query: 45 LYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSV 224
+ E + LMMG+ + + +V +GN+ + G+ ++ K+ T+++ +N + M+F V
Sbjct: 476 IQEAELHSLYLMMGQGLTPVTEVKAGNVVAIRGLGPYISKSATLSSTRNCWPLASMEFQV 535
Query: 225 SPVVRVAVEP 254
SP +RVA+EP
Sbjct: 536 SPTLRVAIEP 545
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 47.6 bits (108), Expect = 5e-06
Identities = 25/72 (34%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
Frame = +2
Query: 263 ADLPKLVEGLKRLAKSDPMVQCI-NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKS 439
AD+ K+ GL +LA+ DP +EE + ++ G GELHLEI + L+ + +
Sbjct: 505 ADIDKMATGLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRLKREFK-VEANVG 563
Query: 440 DPVVSYRETVAE 475
P V+YRE++++
Sbjct: 564 APQVNYRESISK 575
Score = 30.7 bits (66), Expect = 0.67
Identities = 27/82 (32%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +3
Query: 18 NFTPGKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTG-TITTFKNA 194
N GKKE I R + M E ++ +G+I L G+ + TG T++ +N
Sbjct: 430 NANKGKKE-----RIGRLLEMHANSREDVKVALTGDIIALAGLKDTI--TGETLSDPENP 482
Query: 195 HNMKVMKFSVSPVVRVAVEPKT 260
++ M F PV++VA+EPKT
Sbjct: 483 VVLERMDFP-DPVIKVAIEPKT 503
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 44.8 bits (101), Expect = 4e-05
Identities = 21/68 (30%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +2
Query: 284 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYR 460
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 461 ETVAEESD 484
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 44.8 bits (101), Expect = 4e-05
Identities = 21/68 (30%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
Frame = +2
Query: 284 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYR 460
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 461 ETVAEESD 484
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At5g05160.1 68418.m00549 leucine-rich repeat transmembrane protein
kinase, putative
Length = 640
Score = 29.5 bits (63), Expect = 1.5
Identities = 24/80 (30%), Positives = 39/80 (48%), Gaps = 9/80 (11%)
Frame = +2
Query: 137 RWSRSVLSQDWYHHHFQ-ECPQHEG--DEIQCITSRACRC*AQNPADLPK------LVEG 289
RW RSV+ ++W F E + + +E+ + A C A+NP PK ++E
Sbjct: 556 RWVRSVVREEWTAEVFDVELLKFQNIEEEMVQMLQLALACVARNPESRPKMEEVARMIED 615
Query: 290 LKRLAKSDPMVQCINEESGE 349
++RL +S + Q N S E
Sbjct: 616 VRRLDQSQQLQQ--NRTSSE 633
>At1g04650.1 68414.m00462 hypothetical protein
Length = 936
Score = 29.1 bits (62), Expect = 2.0
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = -3
Query: 197 VGILESGDGTSLD*ELIDSNETTDVTR-GHILNSFNITSHHKDCTLDSLLIQVLFLSRCK 21
V + + G S + D E T V G +LN+ +T + LDSL + CK
Sbjct: 313 VSLSQQTHGKSASEVMTDLLEKTTVDLLGALLNAAELTQEFRLTLLDSLFVDEFSNQICK 372
Query: 20 VWSHDA 3
SHD+
Sbjct: 373 KQSHDS 378
>At5g59740.1 68418.m07488 UDP-galactose/UDP-glucose
transporter-related weak similarity to
UDP-galactose/UDP-glucose transporter [Arabidopsis
thaliana] GI:22651763
Length = 344
Score = 28.7 bits (61), Expect = 2.7
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -3
Query: 125 VTRGHILNSFNITSHHKDCTLDSLLIQVL 39
+ +GH+L + + S H+DC LD L+ +
Sbjct: 228 ILQGHLLPAVDFVSLHRDCLLDIALLSTV 256
>At5g17300.1 68418.m02026 myb family transcription factor similar to
CCA1 [Arabidopsis thaliana] GI:4090569; contains Pfam
profile PF00249: Myb-like DNA-binding domain
Length = 387
Score = 28.3 bits (60), Expect = 3.6
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -2
Query: 480 DSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPAT 355
D+ + S T GS+ L + + S +RSL +S S PA T
Sbjct: 154 DTQSPTSVLSTVGSEALCSLDSSSPNRSLSPVSSASPPAALT 195
>At3g46180.1 68416.m04997 UDP-galactose/UDP-glucose
transporter-related contains weak similarity to
UDP-galactose/UDP-glucose transporter (GI:22651763)
[Arabidopsis thaliana]
Length = 347
Score = 28.3 bits (60), Expect = 3.6
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = -3
Query: 125 VTRGHILNSFNITSHHKDCTLDSLLIQVL 39
+ +GH+L + + S H+DC D L+ +
Sbjct: 229 ILQGHLLPAVDFVSRHRDCLFDIALLSTV 257
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 3.6
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +2
Query: 329 INEESGEHIVAGAGELHLEICLKDLEEDH 415
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 4.7
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 362 GAGELHLEICLKDLEEDHACIPIKK 436
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 27.9 bits (59), Expect = 4.7
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -1
Query: 466 GLTVRHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 350
G TV+ DRV W +++ +++TD +EF N+
Sbjct: 348 GGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At3g59130.1 68416.m06592 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 329
Score = 27.5 bits (58), Expect = 6.2
Identities = 14/52 (26%), Positives = 24/52 (46%)
Frame = +2
Query: 131 WSRWSRSVLSQDWYHHHFQECPQHEGDEIQCITSRACRC*AQNPADLPKLVE 286
+ + S S++ W+ HHF + ++EG +AC NP D +E
Sbjct: 167 YKKISGSLIEHCWHQHHFLKLEKYEGTRDSKKQCQACMR-PVNPHDFYSCIE 217
>At1g05055.1 68414.m00506 basic transcription factor 2, 44kD
subunit-related contains weak similarity to
Swiss-Prot:Q13888 TFIIH basal transcription factor
complex p44 subunit (Basic transcription factor 2 44 kDa
subunit, BTF2-p44, General transcription factor IIH
polypeptide 2) [Homo sapiens]
Length = 421
Score = 27.5 bits (58), Expect = 6.2
Identities = 24/91 (26%), Positives = 42/91 (46%), Gaps = 6/91 (6%)
Frame = +2
Query: 173 HHHFQECPQHEGDEIQCITSRACRC*AQNPADLPKLVEGLKRLAKSDPMVQCINEES--G 346
H H + P + E+ + S C C +P D+ + ++ K+ +K V ++ E
Sbjct: 174 HEHLNQVPSYGHREVLILYSALCTC---DPGDIMETIQKCKK-SKLRCSVIGLSAEMFIC 229
Query: 347 EHIVAGAGELHL----EICLKDLEEDHACIP 427
+H+ G L+ E+ LKDL +HA P
Sbjct: 230 KHLCQETGGLYSVAVDEVHLKDLLLEHAPPP 260
>At1g27740.1 68414.m03390 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 258
Score = 27.1 bits (57), Expect = 8.3
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 1 LASWDQTLHLERKRTCMRRLSSVQSL 78
L SWDQ+ HL+ + R+L V++L
Sbjct: 81 LDSWDQSHHLQETSSLKRKLLDVENL 106
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,501,418
Number of Sequences: 28952
Number of extensions: 260661
Number of successful extensions: 753
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 729
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 746
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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