BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20078 (770 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 50 3e-07 SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 35 0.011 SPAC22F3.09c |res2|mcs1, pct1|MBF transcription factor complex s... 30 0.42 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 29 0.56 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.74 SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 28 1.7 SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 27 2.2 SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.9 SPCC1739.10 |mug33||conserved fungal protein|Schizosaccharomyces... 26 6.9 SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 9.1 SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 25 9.1 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 50.4 bits (115), Expect = 3e-07 Identities = 25/68 (36%), Positives = 39/68 (57%) Frame = +2 Query: 314 TGFTPNQIERLYSRFTSLDKNDCGTLSREDFLRIPELAINPLSERIVQSFFAESHDDRVN 493 + F+ +IER+ RF +D N G++ R +FL IP +A NPL+ R+ S E V+ Sbjct: 16 SSFSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLASRLF-SVVDEDGGGDVD 74 Query: 494 FLQFMGFL 517 F +F+ L Sbjct: 75 FQEFINSL 82 Score = 40.7 bits (91), Expect = 2e-04 Identities = 17/29 (58%), Positives = 22/29 (75%) Frame = +1 Query: 565 REEKLRFAFSMYDLDSDGKISRDELLAIL 651 +EEKL+FAF +YD+D DG IS EL +L Sbjct: 91 KEEKLKFAFKIYDIDRDGYISNGELYLVL 119 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 35.1 bits (77), Expect = 0.011 Identities = 15/27 (55%), Positives = 22/27 (81%) Frame = +1 Query: 571 EKLRFAFSMYDLDSDGKISRDELLAIL 651 +KL +AF +YDLD++G IS DE+L I+ Sbjct: 99 DKLIWAFQLYDLDNNGLISYDEMLRIV 125 >SPAC22F3.09c |res2|mcs1, pct1|MBF transcription factor complex subunit Res2|Schizosaccharomyces pombe|chr 1|||Manual Length = 657 Score = 29.9 bits (64), Expect = 0.42 Identities = 19/65 (29%), Positives = 33/65 (50%), Gaps = 1/65 (1%) Frame = +2 Query: 263 KSSLLLREEEIAQIQEETGFTPNQIERLYSRFTSLDKND-CGTLSREDFLRIPELAINPL 439 +S L +EE++ + T N+I R Y T L KN+ + S E+ +R + L Sbjct: 469 ESQLREKEEDLIRANRLKQDTLNEISRTYQELTFLQKNNPTYSQSMENLIREAQETYQQL 528 Query: 440 SERIV 454 S+R++ Sbjct: 529 SKRLL 533 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 29.5 bits (63), Expect = 0.56 Identities = 19/62 (30%), Positives = 28/62 (45%) Frame = +2 Query: 323 TPNQIERLYSRFTSLDKNDCGTLSREDFLRIPELAINPLSERIVQSFFAESHDDRVNFLQ 502 T +QI+ L F LDK+ G + RED + SE + F ES + +N Sbjct: 43 TSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMF-ESINPPINLAA 101 Query: 503 FM 508 F+ Sbjct: 102 FL 103 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 29.1 bits (62), Expect = 0.74 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = +1 Query: 544 RENKLNCREEKLRFAFSMYDLDSDGKISRDELLAIL 651 +E N +++L +DLD DG+I+ E +AI+ Sbjct: 137 KELNENIDDQELEAMIEEFDLDQDGEINEQEFIAIM 172 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 27.9 bits (59), Expect = 1.7 Identities = 11/26 (42%), Positives = 18/26 (69%) Frame = +1 Query: 574 KLRFAFSMYDLDSDGKISRDELLAIL 651 + R AFS++D D DG I+ +EL ++ Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVM 38 >SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase Alg10|Schizosaccharomyces pombe|chr 1|||Manual Length = 445 Score = 27.5 bits (58), Expect = 2.2 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -1 Query: 302 FVQFPPLSTTKTTYFPLSVKSLLTFVPRPLI 210 F+ L T T+F L ++LT VP PL+ Sbjct: 354 FLDISKLQMTSLTFFLLISTTILTLVPAPLV 384 >SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 25.8 bits (54), Expect = 6.9 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -1 Query: 713 GSFSNTRQLFLTNIGSNHPCASIARSSS 630 G S+TR+ F +++GS+ P SSS Sbjct: 328 GHSSSTRRPFFSDVGSSQPAEEFVGSSS 355 >SPCC1739.10 |mug33||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 336 Score = 25.8 bits (54), Expect = 6.9 Identities = 14/36 (38%), Positives = 22/36 (61%) Frame = -3 Query: 393 DNVPQSFLSSDVNRE*RRSI*LGVNPVSSCICAISS 286 ++ F +S + R+ R S L + PVS+CIC IS+ Sbjct: 68 EHAKDGFRTSVIVRQ-RASYGLVIVPVSACICLIST 102 >SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 25.4 bits (53), Expect = 9.1 Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 2/57 (3%) Frame = +2 Query: 245 SQKVGN-KSSLLLREEEIAQIQEETGFTPNQIERLYSRFTSLDK-NDCGTLSREDFL 409 ++++ N K+S+ +EEI +QE+ NQ L R + D LS ED L Sbjct: 135 AEEISNLKTSITHLDEEIQILQEKLAIVTNQRNTLVKRLQTYDNLEKKKALSMEDRL 191 >SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.4 bits (53), Expect = 9.1 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = -1 Query: 698 TRQLFLTNIGSNHPCASIARSSSLDILPSLSR 603 ++Q +T + N P S+ SSS D +P+++R Sbjct: 138 SKQKGITVVFRNDPLESVRVSSSFDSIPAIAR 169 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,286,519 Number of Sequences: 5004 Number of extensions: 70566 Number of successful extensions: 198 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 186 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 198 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 371330890 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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