BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20074 (638 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G6.04 |tim8||TIM22 inner membrane protein import complex s... 49 7e-07 SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex... 33 0.046 SPCC417.11c |||glutamate-1-semialdehyde 2,1-aminomutaseaminotran... 26 5.3 SPCC126.05c |mrpl17||mitochondrial ribosomal protein subunit L17... 25 9.2 SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 25 9.2 SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 25 9.2 SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch... 25 9.2 >SPAC13G6.04 |tim8||TIM22 inner membrane protein import complex subunit Tim8|Schizosaccharomyces pombe|chr 1|||Manual Length = 98 Score = 48.8 bits (111), Expect = 7e-07 Identities = 22/47 (46%), Positives = 26/47 (55%) Frame = +2 Query: 125 ELQDFLIQEKQKAQFQAQIHEFNSFCWDKCIEKPGAKLDSRTENVSQ 265 EL F+ E+QK + Q IH+F S CW KCI G KLD E Q Sbjct: 19 ELSKFIESEQQKVKLQQAIHQFTSTCWPKCIGNIGNKLDKSEEQCLQ 65 Score = 34.7 bits (76), Expect = 0.011 Identities = 16/32 (50%), Positives = 22/32 (68%) Frame = +1 Query: 244 SDRKCITNCVERFIDTSLLITNRFAQMLEKGG 339 S+ +C+ NCVERF+D + I R+A LEK G Sbjct: 59 SEEQCLQNCVERFLDCNFHIIKRYA--LEKFG 88 >SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex subunit Tim13|Schizosaccharomyces pombe|chr 1|||Manual Length = 95 Score = 32.7 bits (71), Expect = 0.046 Identities = 17/47 (36%), Positives = 23/47 (48%) Frame = +2 Query: 101 PSPKGADSELQDFLIQEKQKAQFQAQIHEFNSFCWDKCIEKPGAKLD 241 PS + S + QE AQ I + N C+DKCI +PG+ D Sbjct: 12 PSSEDKKSIFMKQIRQELAVAQAGELISKINENCFDKCIPEPGSTFD 58 Score = 25.8 bits (54), Expect = 5.3 Identities = 5/30 (16%), Positives = 20/30 (66%) Frame = +1 Query: 244 SDRKCITNCVERFIDTSLLITNRFAQMLEK 333 +++ C++ C+ER++D +++ + +++ Sbjct: 60 NEKSCVSKCMERYMDAWNIVSRTYISRMQR 89 >SPCC417.11c |||glutamate-1-semialdehyde 2,1-aminomutaseaminotransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 435 Score = 25.8 bits (54), Expect = 5.3 Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 1/37 (2%) Frame = +2 Query: 122 SELQDF-LIQEKQKAQFQAQIHEFNSFCWDKCIEKPG 229 SE+ D L +EK +AQF A+ + N F C PG Sbjct: 3 SEISDLSLAKEKSEAQFIARNPKSNDFHCKACDSLPG 39 >SPCC126.05c |mrpl17||mitochondrial ribosomal protein subunit L17|Schizosaccharomyces pombe|chr 3|||Manual Length = 268 Score = 25.0 bits (52), Expect = 9.2 Identities = 9/46 (19%), Positives = 26/46 (56%) Frame = +2 Query: 143 IQEKQKAQFQAQIHEFNSFCWDKCIEKPGAKLDSRTENVSQTVWKD 280 I +Q ++F+ I+++N+ W++ + A+ + +V + W++ Sbjct: 52 ILTRQPSEFEKSIYKYNAELWNELSDPLPAEFYFKKGSVGEKDWQE 97 >SPBC11G11.02c |end3||actin cortical patch component End3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 25.0 bits (52), Expect = 9.2 Identities = 12/37 (32%), Positives = 18/37 (48%) Frame = -3 Query: 567 DMHYSLCVTNNNYMLKFRFPSLNMQNNYYYTTTVKQS 457 D+ YSL + + + S N NNYY + T+ S Sbjct: 215 DVPYSLKASFKRGNIDYNLDSYNSTNNYYSSPTMAPS 251 >SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 762 Score = 25.0 bits (52), Expect = 9.2 Identities = 9/21 (42%), Positives = 14/21 (66%) Frame = -3 Query: 510 PSLNMQNNYYYTTTVKQSIVN 448 PS ++ + YYT KQS++N Sbjct: 372 PSFDVWDREYYTARYKQSLIN 392 >SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1092 Score = 25.0 bits (52), Expect = 9.2 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +2 Query: 104 SPKGADSELQDFLIQEKQKAQFQAQIHEFN 193 SPKG ++D++ K F AQ EFN Sbjct: 100 SPKGYSDLIRDYVDYLLDKLSFHAQHPEFN 129 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,568,128 Number of Sequences: 5004 Number of extensions: 51843 Number of successful extensions: 153 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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