BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20041 (616 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 29 0.71 SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyce... 28 1.2 SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 27 2.2 SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 27 2.8 SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ... 26 3.8 SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ... 26 3.8 SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit ... 25 6.6 SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 25 6.6 SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ... 25 8.7 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 28.7 bits (61), Expect = 0.71 Identities = 27/106 (25%), Positives = 48/106 (45%), Gaps = 6/106 (5%) Frame = +2 Query: 74 RHRSVDRDVKARG---DEHARMSDSQAHGEAMSDRRSFFYPDSSSGSEEYNRDAEERRRV 244 R R + R+ + R +E R +A A S R++ P S+ + R+ +E+ R+ Sbjct: 46 RQRKLQRERELRQKYEEEQRRQQAMEAKRIAASTRQTSERPPLSAEEAKRIREVKEKDRL 105 Query: 245 SQSVWSIEQAPRSYNAVRGVFV**PDAIDLFSS---RTSEKRSEAP 373 + PRSYN + P + SS ++ +KRS++P Sbjct: 106 ESKKNERQGKPRSYNELLRQASSAPAVNETSSSGLLQSKDKRSQSP 151 >SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 277 Score = 27.9 bits (59), Expect = 1.2 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -1 Query: 319 GLLDEHAPDCIVGARGLFDTPDALRDSPTL 230 G LDEHAP + GA+ D ++ P+L Sbjct: 179 GFLDEHAPSLLEGAQASIDKNRPGKNQPSL 208 >SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1011 Score = 27.1 bits (57), Expect = 2.2 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +2 Query: 194 SSGSEEYNRDAEERRRVSQSVWSIEQAPRSYN 289 ++G+E R+AEERRR + + Q S N Sbjct: 923 NAGTEAMRREAEERRRAEEEEYERSQQESSSN 954 >SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 26.6 bits (56), Expect = 2.8 Identities = 12/33 (36%), Positives = 16/33 (48%) Frame = +1 Query: 262 YRTGPALLQCSPGRVRLVTRCHRPLQLKNLRKT 360 YR G L QCSP V + + K ++KT Sbjct: 743 YRRGRLLQQCSPSSVTKYLKYEEKFKRKRMKKT 775 >SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 26.2 bits (55), Expect = 3.8 Identities = 12/21 (57%), Positives = 13/21 (61%) Frame = -1 Query: 157 CLTMCLRVRHTRVLVSASFYI 95 CLT+CL VR T L AS I Sbjct: 470 CLTICLAVRETMPLTLASLII 490 >SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 26.2 bits (55), Expect = 3.8 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = +3 Query: 180 SILIAAVARKSTTETRRSVGESLKASGVSNRPRAPT 287 S +I+ A + +T R+ S + SGVS+ PRA T Sbjct: 162 SSVISPAASLNRFQTPRAAAISKRESGVSSEPRART 197 >SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit Bdp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 507 Score = 25.4 bits (53), Expect = 6.6 Identities = 10/29 (34%), Positives = 15/29 (51%) Frame = +2 Query: 209 EYNRDAEERRRVSQSVWSIEQAPRSYNAV 295 EY + RRV+ + W Q P +NA+ Sbjct: 343 EYVEENSLSRRVTSATWGNRQKPEKWNAM 371 >SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 944 Score = 25.4 bits (53), Expect = 6.6 Identities = 16/60 (26%), Positives = 22/60 (36%) Frame = +2 Query: 65 PPRRHRSVDRDVKARGDEHARMSDSQAHGEAMSDRRSFFYPDSSSGSEEYNRDAEERRRV 244 PP RH+S H R DS+ A RS + + + DAE + V Sbjct: 139 PPSRHQSPPPSSVLASQRHHRRHDSELEEFARRASRSLSFSMNGTPQRRMTFDAEAWKNV 198 >SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase Cho2|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 25.0 bits (52), Expect = 8.7 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = -3 Query: 152 HHVPESQTYARARLRELLHHGPHYGASEAEIYTSLSL 42 +H+P + TY LR H +G IYTS+S+ Sbjct: 478 YHLPSNWTYGLVSLR----HALGFGLIALHIYTSVSI 510 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,542,409 Number of Sequences: 5004 Number of extensions: 50957 Number of successful extensions: 139 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 269634532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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