BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20041
(616 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 29 0.71
SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyce... 28 1.2
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 27 2.2
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 27 2.8
SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ... 26 3.8
SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ... 26 3.8
SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit ... 25 6.6
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 25 6.6
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ... 25 8.7
>SPCC1393.02c |||non-specific DNA binding protein Spt2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 406
Score = 28.7 bits (61), Expect = 0.71
Identities = 27/106 (25%), Positives = 48/106 (45%), Gaps = 6/106 (5%)
Frame = +2
Query: 74 RHRSVDRDVKARG---DEHARMSDSQAHGEAMSDRRSFFYPDSSSGSEEYNRDAEERRRV 244
R R + R+ + R +E R +A A S R++ P S+ + R+ +E+ R+
Sbjct: 46 RQRKLQRERELRQKYEEEQRRQQAMEAKRIAASTRQTSERPPLSAEEAKRIREVKEKDRL 105
Query: 245 SQSVWSIEQAPRSYNAVRGVFV**PDAIDLFSS---RTSEKRSEAP 373
+ PRSYN + P + SS ++ +KRS++P
Sbjct: 106 ESKKNERQGKPRSYNELLRQASSAPAVNETSSSGLLQSKDKRSQSP 151
>SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 277
Score = 27.9 bits (59), Expect = 1.2
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 319 GLLDEHAPDCIVGARGLFDTPDALRDSPTL 230
G LDEHAP + GA+ D ++ P+L
Sbjct: 179 GFLDEHAPSLLEGAQASIDKNRPGKNQPSL 208
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 27.1 bits (57), Expect = 2.2
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 194 SSGSEEYNRDAEERRRVSQSVWSIEQAPRSYN 289
++G+E R+AEERRR + + Q S N
Sbjct: 923 NAGTEAMRREAEERRRAEEEEYERSQQESSSN 954
>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +1
Query: 262 YRTGPALLQCSPGRVRLVTRCHRPLQLKNLRKT 360
YR G L QCSP V + + K ++KT
Sbjct: 743 YRRGRLLQQCSPSSVTKYLKYEEKFKRKRMKKT 775
>SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 570
Score = 26.2 bits (55), Expect = 3.8
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -1
Query: 157 CLTMCLRVRHTRVLVSASFYI 95
CLT+CL VR T L AS I
Sbjct: 470 CLTICLAVRETMPLTLASLII 490
>SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 26.2 bits (55), Expect = 3.8
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = +3
Query: 180 SILIAAVARKSTTETRRSVGESLKASGVSNRPRAPT 287
S +I+ A + +T R+ S + SGVS+ PRA T
Sbjct: 162 SSVISPAASLNRFQTPRAAAISKRESGVSSEPRART 197
>SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit
Bdp1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 507
Score = 25.4 bits (53), Expect = 6.6
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +2
Query: 209 EYNRDAEERRRVSQSVWSIEQAPRSYNAV 295
EY + RRV+ + W Q P +NA+
Sbjct: 343 EYVEENSLSRRVTSATWGNRQKPEKWNAM 371
>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 25.4 bits (53), Expect = 6.6
Identities = 16/60 (26%), Positives = 22/60 (36%)
Frame = +2
Query: 65 PPRRHRSVDRDVKARGDEHARMSDSQAHGEAMSDRRSFFYPDSSSGSEEYNRDAEERRRV 244
PP RH+S H R DS+ A RS + + + DAE + V
Sbjct: 139 PPSRHQSPPPSSVLASQRHHRRHDSELEEFARRASRSLSFSMNGTPQRRMTFDAEAWKNV 198
>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
Cho2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 905
Score = 25.0 bits (52), Expect = 8.7
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -3
Query: 152 HHVPESQTYARARLRELLHHGPHYGASEAEIYTSLSL 42
+H+P + TY LR H +G IYTS+S+
Sbjct: 478 YHLPSNWTYGLVSLR----HALGFGLIALHIYTSVSI 510
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,542,409
Number of Sequences: 5004
Number of extensions: 50957
Number of successful extensions: 139
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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