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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20041
         (616 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac...    29   0.71 
SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyce...    28   1.2  
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha...    27   2.2  
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    27   2.8  
SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ...    26   3.8  
SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ...    26   3.8  
SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit ...    25   6.6  
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo...    25   6.6  
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ...    25   8.7  

>SPCC1393.02c |||non-specific DNA binding protein Spt2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 406

 Score = 28.7 bits (61), Expect = 0.71
 Identities = 27/106 (25%), Positives = 48/106 (45%), Gaps = 6/106 (5%)
 Frame = +2

Query: 74  RHRSVDRDVKARG---DEHARMSDSQAHGEAMSDRRSFFYPDSSSGSEEYNRDAEERRRV 244
           R R + R+ + R    +E  R    +A   A S R++   P  S+   +  R+ +E+ R+
Sbjct: 46  RQRKLQRERELRQKYEEEQRRQQAMEAKRIAASTRQTSERPPLSAEEAKRIREVKEKDRL 105

Query: 245 SQSVWSIEQAPRSYNAVRGVFV**PDAIDLFSS---RTSEKRSEAP 373
                  +  PRSYN +       P   +  SS   ++ +KRS++P
Sbjct: 106 ESKKNERQGKPRSYNELLRQASSAPAVNETSSSGLLQSKDKRSQSP 151


>SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 277

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -1

Query: 319 GLLDEHAPDCIVGARGLFDTPDALRDSPTL 230
           G LDEHAP  + GA+   D     ++ P+L
Sbjct: 179 GFLDEHAPSLLEGAQASIDKNRPGKNQPSL 208


>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
            1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1011

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +2

Query: 194  SSGSEEYNRDAEERRRVSQSVWSIEQAPRSYN 289
            ++G+E   R+AEERRR  +  +   Q   S N
Sbjct: 923  NAGTEAMRREAEERRRAEEEEYERSQQESSSN 954


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 26.6 bits (56), Expect = 2.8
 Identities = 12/33 (36%), Positives = 16/33 (48%)
 Frame = +1

Query: 262 YRTGPALLQCSPGRVRLVTRCHRPLQLKNLRKT 360
           YR G  L QCSP  V    +     + K ++KT
Sbjct: 743 YRRGRLLQQCSPSSVTKYLKYEEKFKRKRMKKT 775


>SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 570

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 12/21 (57%), Positives = 13/21 (61%)
 Frame = -1

Query: 157 CLTMCLRVRHTRVLVSASFYI 95
           CLT+CL VR T  L  AS  I
Sbjct: 470 CLTICLAVRETMPLTLASLII 490


>SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 736

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 14/36 (38%), Positives = 21/36 (58%)
 Frame = +3

Query: 180 SILIAAVARKSTTETRRSVGESLKASGVSNRPRAPT 287
           S +I+  A  +  +T R+   S + SGVS+ PRA T
Sbjct: 162 SSVISPAASLNRFQTPRAAAISKRESGVSSEPRART 197


>SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit
           Bdp1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 507

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 10/29 (34%), Positives = 15/29 (51%)
 Frame = +2

Query: 209 EYNRDAEERRRVSQSVWSIEQAPRSYNAV 295
           EY  +    RRV+ + W   Q P  +NA+
Sbjct: 343 EYVEENSLSRRVTSATWGNRQKPEKWNAM 371


>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 944

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 16/60 (26%), Positives = 22/60 (36%)
 Frame = +2

Query: 65  PPRRHRSVDRDVKARGDEHARMSDSQAHGEAMSDRRSFFYPDSSSGSEEYNRDAEERRRV 244
           PP RH+S           H R  DS+    A    RS  +  + +       DAE  + V
Sbjct: 139 PPSRHQSPPPSSVLASQRHHRRHDSELEEFARRASRSLSFSMNGTPQRRMTFDAEAWKNV 198


>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
           Cho2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 905

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = -3

Query: 152 HHVPESQTYARARLRELLHHGPHYGASEAEIYTSLSL 42
           +H+P + TY    LR    H   +G     IYTS+S+
Sbjct: 478 YHLPSNWTYGLVSLR----HALGFGLIALHIYTSVSI 510


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,542,409
Number of Sequences: 5004
Number of extensions: 50957
Number of successful extensions: 139
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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