Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20037
(704 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 99 3e-22
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 79 8e-16
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 61 2e-10
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 48 2e-06
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 2e-05
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.004
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 36 0.007
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 35 0.013
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 28 1.5
SPBC31F10.14c |hip3|hir3|HIRA interacting protein Hip3|Schizosac... 26 4.6
SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizos... 26 4.6
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 99 bits (238), Expect = 3e-22
Identities = 44/83 (53%), Positives = 59/83 (71%), Gaps = 2/83 (2%)
Frame = +3
Query: 264 VFDTTKKVLVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKIDATANELEHTKITSF 443
V D TK VLVEFYAPWCGHCK L P Y+KL E + +D +V++AKIDAT N++ I+ F
Sbjct: 369 VMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGF 427
Query: 444 PTIKLYSKDNQVHD--YNGERTL 506
PTI + +++V+ Y G+RTL
Sbjct: 428 PTIMFFKANDKVNPVRYEGDRTL 450
Score = 64.9 bits (151), Expect = 1e-11
Identities = 33/98 (33%), Positives = 51/98 (52%), Gaps = 6/98 (6%)
Frame = +3
Query: 279 KKVLVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKIDAT--ANELEHTKITSFPTI 452
K ++V+FYAPWCGHCK L P Y+ + E D + + ++D T + I +PT+
Sbjct: 40 KVLMVKFYAPWCGHCKALAPEYESAADELEK-DGISLVEVDCTEEGDLCSEYSIRGYPTL 98
Query: 453 KLYSKDNQVHDYNGERTLAASPSSLR----PTVKAPSQ 554
++ Q+ Y+G R A +R PTVK S+
Sbjct: 99 NVFKNGKQISQYSGPRKHDALVKYMRKQLLPTVKPISK 136
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 78.6 bits (185), Expect = 8e-16
Identities = 43/100 (43%), Positives = 60/100 (60%), Gaps = 4/100 (4%)
Frame = +3
Query: 219 PPNPSKCSSPPT*RSVFDTTKKVLVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKI 398
P N + S + V D K VLVEFYA WCG+CK+L P Y+ LG+ F+N+ +V I KI
Sbjct: 139 PSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKI 198
Query: 399 --DATANELEHTKITSFPTIKLYSKDNQVHD--YNGERTL 506
D A+ ++ SFPTIK + KD++ Y G+R+L
Sbjct: 199 NADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSL 238
Score = 72.9 bits (171), Expect = 4e-14
Identities = 32/66 (48%), Positives = 43/66 (65%), Gaps = 2/66 (3%)
Frame = +3
Query: 279 KKVLVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKIDATANE--LEHTKITSFPTI 452
K L+EFYA WCGHCK L P+Y++LG FE+ +DV+I KIDA + + IT FPT+
Sbjct: 40 KGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTL 99
Query: 453 KLYSKD 470
+ D
Sbjct: 100 IWFPPD 105
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 60.9 bits (141), Expect = 2e-10
Identities = 34/79 (43%), Positives = 44/79 (55%), Gaps = 7/79 (8%)
Frame = +3
Query: 288 LVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKIDATANE--LEHTKITSFPTIKLY 461
LV FYAPWCG+CK+LVP Y KL + + V DA N ++ FPTIKL
Sbjct: 52 LVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLV 111
Query: 462 ---SKDNQVH--DYNGERT 503
SK + + DYNG+R+
Sbjct: 112 YPSSKGSSLSSTDYNGDRS 130
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 47.6 bits (108), Expect = 2e-06
Identities = 24/96 (25%), Positives = 47/96 (48%)
Frame = +3
Query: 258 RSVFDTTKKVLVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKIDATANELEHTKIT 437
+S+ K V+V+F+A WCG CK + P +++ + +D I +D + +
Sbjct: 12 KSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTY-SDATFIKVDVDQLSEIAAEAGVH 70
Query: 438 SFPTIKLYSKDNQVHDYNGERTLAASPSSLRPTVKA 545
+ P+ LY ++ E + A+P+ L ++KA
Sbjct: 71 AMPSFFLYKNGEKI-----EEIVGANPAKLEASIKA 101
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 44.4 bits (100), Expect = 2e-05
Identities = 21/67 (31%), Positives = 33/67 (49%)
Frame = +3
Query: 279 KKVLVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKIDATANELEHTKITSFPTIKL 458
K +V+FYA WCG CK L P +KL E I D ++ + + + PT+ L
Sbjct: 36 KVTVVDFYADWCGPCKYLKPFLEKLSEQ-NQKASFIAVNADKFSDIAQKNGVYALPTMVL 94
Query: 459 YSKDNQV 479
+ K ++
Sbjct: 95 FRKGQEL 101
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 36.3 bits (80), Expect = 0.004
Identities = 20/84 (23%), Positives = 35/84 (41%), Gaps = 1/84 (1%)
Frame = +3
Query: 291 VEFYAPWCGHCKQLVPIYDKLGEHFENDDDVII-AKIDATANELEHTKITSFPTIKLYSK 467
V+ YA WCG CK + P++ +L + + V +D + + PT +
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 468 DNQVHDYNGERTLAASPSSLRPTV 539
Q+ G A+P +L+ V
Sbjct: 84 GKQIDMLTG-----ANPQALKEKV 102
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 35.5 bits (78), Expect = 0.007
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +3
Query: 285 VLVEFYAPWCGHCKQLVPIYDKLGEHFENDDDVIIAKIDA 404
+L+ FYAPW CKQ+ ++D+ + +N + KI+A
Sbjct: 23 ILLNFYAPWAAPCKQMNQVFDQFAKDTKN---AVFLKIEA 59
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 34.7 bits (76), Expect = 0.013
Identities = 17/68 (25%), Positives = 32/68 (47%), Gaps = 3/68 (4%)
Frame = +3
Query: 291 VEFYAPWCGHCKQLVPIYDKLGEHFEND---DDVIIAKIDATANELEHTKITSFPTIKLY 461
+++Y P CG CK+L P++D + E + + ++D + I + PT+ LY
Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLY 106
Query: 462 SKDNQVHD 485
V +
Sbjct: 107 QNGEIVEE 114
>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
Dna2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1398
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/42 (28%), Positives = 19/42 (45%)
Frame = -2
Query: 142 NSXRRQLITAGLVFGHVLFKGDQTGRWNLILLHTEELQDPLV 17
N R + +G G +F D+ G W+L H +QD +
Sbjct: 791 NGYPRNFLHSGFSVGERVFISDEHGHWSLAKGHIVHIQDSCI 832
>SPBC31F10.14c |hip3|hir3|HIRA interacting protein
Hip3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1630
Score = 26.2 bits (55), Expect = 4.6
Identities = 16/52 (30%), Positives = 24/52 (46%)
Frame = +3
Query: 405 TANELEHTKITSFPTIKLYSKDNQVHDYNGERTLAASPSSLRPTVKAPSQYL 560
+AN LEH ++ ++L S+ N ++ E S LRP P YL
Sbjct: 451 SANRLEHMQVPDGYLLQLLSEVNSLNMVPAELATFFVESMLRPRKLEPPFYL 502
>SPBC1539.05 |cog3||Golgi transport complex subunit Cog3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/48 (29%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
Frame = +2
Query: 8 EDEHQRILEFFGMKKDEVPSARLI----ALEQDMAKYKPSSNELSPXA 139
ED ++++EF E+ L A+E D+ +Y P+ +L+P A
Sbjct: 415 EDVDKKVVEFLNPLVLELQERLLFVVQTAIETDIQRYSPTEEDLNPIA 462
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,545,847
Number of Sequences: 5004
Number of extensions: 48654
Number of successful extensions: 173
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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