BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20036 (591 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.38 SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 27 2.7 SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 26 3.6 SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 26 3.6 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 4.7 SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 25 6.2 SPBC24C6.10c |||conserved eukaryotic protein|Schizosaccharomyces... 25 8.3 >SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 29.5 bits (63), Expect = 0.38 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = -2 Query: 503 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 402 DLE N +V Q+ G G+FL G FG + FV+ Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444 >SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1237 Score = 26.6 bits (56), Expect = 2.7 Identities = 11/40 (27%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = +1 Query: 115 ELYMSVVIGEY-ETAIAKCSEYLKEKKGEVIKEAVKRLIE 231 ++++++V+ Y A+ EYL+EK I + K ++E Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595 >SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase|Schizosaccharomyces pombe|chr 2|||Manual Length = 362 Score = 26.2 bits (55), Expect = 3.6 Identities = 9/15 (60%), Positives = 10/15 (66%) Frame = +3 Query: 243 EHHGFAYQLWTKDGK 287 EHH F Y WT+ GK Sbjct: 292 EHHDFPYVAWTRIGK 306 >SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 26.2 bits (55), Expect = 3.6 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = -2 Query: 197 SPFFSFRYSEHLAIAVSYSPMTTLIYSSPP 108 +PFF +S HL S S + + YS PP Sbjct: 343 APFFPLGFSPHLNDESSRSRWSNISYSPPP 372 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 25.8 bits (54), Expect = 4.7 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +3 Query: 351 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 443 L+ K+D A K+ DQ H K+ +DK S Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526 >SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr 3|||Manual Length = 1647 Score = 25.4 bits (53), Expect = 6.2 Identities = 12/39 (30%), Positives = 23/39 (58%) Frame = -2 Query: 401 VLLVDQLEGVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 285 V+L+ + G + + L LL +D +++ +V FDDF+ Sbjct: 1284 VVLLQDIHGCLTAWA-RLTGLLVDDCNEVISDVHFDDFI 1321 >SPBC24C6.10c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 25.0 bits (52), Expect = 8.3 Identities = 18/73 (24%), Positives = 35/73 (47%) Frame = -2 Query: 362 FVYELDSLLGEDHSKLDGEVRFDDFLSILCP*LVGESMVFLLPFSIRRFTASLITSPFFS 183 F+ E+D LL + K + E+ FLS + GE + + F ++R + SP Sbjct: 16 FLAEIDELLTIPYEKNELEIGVSKFLSFINK--YGEPYL-ITEFQLQRCMEKFLNSPL-- 70 Query: 182 FRYSEHLAIAVSY 144 ++ E+ +++ Y Sbjct: 71 YQLDENAVLSIFY 83 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,492,852 Number of Sequences: 5004 Number of extensions: 52316 Number of successful extensions: 208 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 201 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 208 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 256184654 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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