BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20034 (519 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 79 3e-16 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 64 1e-11 SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 40 3e-04 SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosacch... 28 0.96 SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog, Rhp... 25 9.0 >SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 937 Score = 79.4 bits (187), Expect = 3e-16 Identities = 35/76 (46%), Positives = 51/76 (67%) Frame = +3 Query: 24 MAQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGRXDSNVYIRMKLKAAEK 203 MA ++ G +A + +LR+Q++ ++S F L I+QVGGR DSNVY+RMK +AA + Sbjct: 1 MALLLEGTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANE 60 Query: 204 IGIAAEHIRLPRDITE 251 GI+ EH+ P DITE Sbjct: 61 AGISCEHVNFPEDITE 76 Score = 59.7 bits (138), Expect = 3e-10 Identities = 33/86 (38%), Positives = 49/86 (56%), Gaps = 1/86 (1%) Frame = +2 Query: 257 LLAKITSLNESPSVHGIIVQMPXXSDHAXDAHRVTDAVSPDKDVDGLNTINEGRVXVGDL 436 LL I NE P+VHGIIVQ+P + H + +T+AV+P+KDVDG N G++ + Sbjct: 79 LLLAIKGFNEDPTVHGIIVQLPLPA-HINE-QIITEAVAPEKDVDGFCETNLGKLTKREG 136 Query: 437 SGXI-PCTPAGCVELIXKTGVTIAGK 511 CTP G + ++ G+ + GK Sbjct: 137 QPLFTACTPKGIMCILKHYGINVQGK 162 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 64.1 bits (149), Expect = 1e-11 Identities = 32/73 (43%), Positives = 47/73 (64%) Frame = +3 Query: 27 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGRXDSNVYIRMKLKAAEKI 206 A I++G +A ++ + ++V L++K F+P LAIVQVG R DSNVY+RMK KAA + Sbjct: 33 ANILNGKLLARKLQRSVAEEVQALKAKDRNFKPALAIVQVGKREDSNVYVRMKEKAARLV 92 Query: 207 GIAAEHIRLPRDI 245 GI ++ P I Sbjct: 93 GIDFKYCPFPETI 105 Score = 62.5 bits (145), Expect = 4e-11 Identities = 31/85 (36%), Positives = 50/85 (58%), Gaps = 1/85 (1%) Frame = +2 Query: 257 LLAKITSLNESPSVHGIIVQMPXXSDHAXDAHRVTDAVSPDKDVDGLNTINEGRVXVGDL 436 LL ++ LN+ +VHG++VQ+P H + VT++++P KDVDG N G + D Sbjct: 110 LLHELKKLNDDHTVHGVLVQLPLPK-HLNE-RTVTESITPPKDVDGFGAFNIGLLAKNDA 167 Query: 437 SG-XIPCTPAGCVELIXKTGVTIAG 508 + PCTP G +EL+ +++AG Sbjct: 168 TPIHYPCTPKGIMELLKDNKISVAG 192 >SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccharomyces pombe|chr 2|||Manual Length = 320 Score = 39.5 bits (88), Expect = 3e-04 Identities = 28/84 (33%), Positives = 42/84 (50%), Gaps = 8/84 (9%) Frame = +2 Query: 269 ITSLNESPSVHGIIVQMPXXSDHAXDAHRVTDAVSPDKDVDGL------NTINEGRVXVG 430 I N PSV+GI++ P +D D + + VSPDKDV+GL N + R Sbjct: 85 IVEANNDPSVNGIMIYFPVFND-GQDQY-LQQVVSPDKDVEGLCHKYVMNMYHNIRHLDP 142 Query: 431 DLS--GXIPCTPAGCVELIXKTGV 496 + + +PCTP V+++ GV Sbjct: 143 EKTKKSILPCTPLAIVKILEYLGV 166 >SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosaccharomyces pombe|chr 1|||Manual Length = 179 Score = 27.9 bits (59), Expect = 0.96 Identities = 14/33 (42%), Positives = 22/33 (66%), Gaps = 1/33 (3%) Frame = +3 Query: 111 SGFEPRLAIVQ-VGGRXDSNVYIRMKLKAAEKI 206 SGFE R+ I++ + DSN+YIR+K + K+ Sbjct: 11 SGFENRIKILEDLMNEFDSNLYIRLKRECDFKL 43 >SPBC342.05 |crb2|rhp9, rhp9|DNA repair protein RAD9 homolog, Rhp9|Schizosaccharomyces pombe|chr 2|||Manual Length = 778 Score = 24.6 bits (51), Expect = 9.0 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = +1 Query: 208 VSPPNISAYQGTSLK*AVSKNHESERISFRSRH 306 V+P NI +QG L + K+ + E SF H Sbjct: 478 VAPVNIRRFQGRDLSFSTLKDMKFEETSFLPSH 510 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,781,467 Number of Sequences: 5004 Number of extensions: 29473 Number of successful extensions: 69 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 61 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 65 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 210309424 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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