BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20034 (519 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd... 77 7e-15 At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 76 2e-14 At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd... 73 1e-13 At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd... 69 2e-12 At5g46570.1 68418.m05734 protein kinase family protein contains ... 31 0.47 At1g64330.1 68414.m07290 myosin heavy chain-related similar to m... 28 4.3 At3g45780.1 68416.m04953 protein kinase / nonphototropic hypocot... 27 7.6 At1g72300.1 68414.m08358 leucine-rich repeat transmembrane prote... 27 7.6 >At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 299 Score = 77.0 bits (181), Expect = 7e-15 Identities = 37/86 (43%), Positives = 57/86 (66%), Gaps = 1/86 (1%) Frame = +2 Query: 257 LLAKITSLNESPSVHGIIVQMPXXSDHAXDAHRVTDAVSPDKDVDGLNTINEGRVXV-GD 433 L++K+ LN +P VHGI+VQ+P H + H + A+S DKDVDG + +N G++ + G Sbjct: 85 LISKVHELNSNPDVHGILVQLPLPK-HINEEH-ILGAISIDKDVDGFHPLNIGKLAMKGR 142 Query: 434 LSGXIPCTPAGCVELIXKTGVTIAGK 511 +PCTP GC+EL+ ++GV I G+ Sbjct: 143 EPLFLPCTPKGCLELLARSGVKIKGQ 168 >At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 360 Score = 75.8 bits (178), Expect = 2e-14 Identities = 36/87 (41%), Positives = 55/87 (63%), Gaps = 1/87 (1%) Frame = +2 Query: 254 KLLAKITSLNESPSVHGIIVQMPXXSDHAXDAHRVTDAVSPDKDVDGLNTINEGRVXV-G 430 ++L ++ N+ PSVHGI+VQ+P S D + +AVS +KDVDG + +N GR+ + G Sbjct: 144 EVLKSVSGFNDDPSVHGILVQLPLPSH--MDEQNILNAVSIEKDVDGFHPLNIGRLAMRG 201 Query: 431 DLSGXIPCTPAGCVELIXKTGVTIAGK 511 +PCTP GC+EL+ + + I GK Sbjct: 202 REPLFVPCTPKGCIELLHRYNIEIKGK 228 Score = 53.2 bits (122), Expect = 1e-07 Identities = 28/75 (37%), Positives = 44/75 (58%) Frame = +3 Query: 27 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGRXDSNVYIRMKLKAAEKI 206 A +I G VA I +++ +V+R++ G P LA++ VG R DS Y+R K KA + + Sbjct: 69 AIVIDGKAVAKKIRDEITIEVSRMKES-IGVIPGLAVILVGDRKDSATYVRNKKKACDSV 127 Query: 207 GIAAEHIRLPRDITE 251 GI + +RL D +E Sbjct: 128 GIKSFEVRLAEDSSE 142 >At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 310 Score = 72.9 bits (171), Expect = 1e-13 Identities = 34/87 (39%), Positives = 54/87 (62%), Gaps = 1/87 (1%) Frame = +2 Query: 254 KLLAKITSLNESPSVHGIIVQMPXXSDHAXDAHRVTDAVSPDKDVDGLNTINEGRVXV-G 430 ++L ++ N+ PSVHG++VQ+P S D + +AVS +KDVDG + +N GR+ + G Sbjct: 94 EVLKYVSGFNDDPSVHGVLVQLPLPSH--MDEQNILNAVSIEKDVDGFHPLNIGRLAMRG 151 Query: 431 DLSGXIPCTPAGCVELIXKTGVTIAGK 511 +PCTP GC+EL+ + + GK Sbjct: 152 REPLFVPCTPKGCIELLHRYNIEFKGK 178 >At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 352 Score = 68.9 bits (161), Expect = 2e-12 Identities = 34/87 (39%), Positives = 55/87 (63%), Gaps = 1/87 (1%) Frame = +2 Query: 254 KLLAKITSLNESPSVHGIIVQMPXXSDHAXDAHRVTDAVSPDKDVDGLNTINEGRVXV-G 430 ++++ + NE S+HGI+VQ+P H ++ ++ + V +KDVDG + +N G + + G Sbjct: 138 QIISVLRKFNEDTSIHGILVQLPLPQ-HLNES-KILNMVRLEKDVDGFHPLNVGNLAMRG 195 Query: 431 DLSGXIPCTPAGCVELIXKTGVTIAGK 511 + CTP GCVEL+ +TGV IAGK Sbjct: 196 REPLFVSCTPKGCVELLIRTGVEIAGK 222 Score = 50.4 bits (115), Expect = 7e-07 Identities = 28/73 (38%), Positives = 41/73 (56%) Frame = +3 Query: 33 IISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGRXDSNVYIRMKLKAAEKIGI 212 +I G +A I + +V +++ K G P LA+V VG + DS Y+R K+KA E+ GI Sbjct: 65 VIDGNVIAEEIRTKIISEVGKMK-KAVGKVPGLAVVLVGEQRDSQTYVRNKIKACEETGI 123 Query: 213 AAEHIRLPRDITE 251 + LP D TE Sbjct: 124 KSVLAELPEDCTE 136 >At5g46570.1 68418.m05734 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 489 Score = 31.1 bits (67), Expect = 0.47 Identities = 19/59 (32%), Positives = 28/59 (47%) Frame = +3 Query: 36 ISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGRXDSNVYIRMKLKAAEKIGI 212 ++G +V I+N ++ LR +GF P IV GG NV R KL+ + I Sbjct: 26 VNGDQVDQEIQNFKEFELNELRKATNGFSPS-CIVSEGGEKAPNVVYRGKLEGNHLVAI 83 >At1g64330.1 68414.m07290 myosin heavy chain-related similar to myosin heavy chain (GI:1850913) [Entamoeba histolytica]; similar to Intracellular protein transport protein USO1 (Swiss-Prot:P25386) [Saccharomyces cerevisiae] Length = 555 Score = 27.9 bits (59), Expect = 4.3 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +3 Query: 45 IEVAGSIENDLRQQVTRLRSKWSGFEPRLA 134 +EVAG E+DL Q++ ++ + G E LA Sbjct: 190 LEVAGETESDLNQKLEDVKKERDGLEAELA 219 >At3g45780.1 68416.m04953 protein kinase / nonphototropic hypocotyl protein 1 (NPH1) / phototropin identical to SP|O48963 Nonphototropic hypocotyl protein 1 (EC 2.7.1.37) (Phototropin) {Arabidopsis thaliana}, cDNA nonphototropic hypocotyl 1 (NPH1) GI:2832240; contains Pfam profiles PF00069:Protein kinase domain and PF00785:PAC motif Length = 996 Score = 27.1 bits (57), Expect = 7.6 Identities = 15/32 (46%), Positives = 16/32 (50%) Frame = -1 Query: 429 PTXTRPSLIVFKPSTSLSGETASVTR*ASXAW 334 P TR SL VF PST L+ V R AW Sbjct: 17 PRDTRGSLEVFNPSTQLTRPDNPVFRPEPPAW 48 >At1g72300.1 68414.m08358 leucine-rich repeat transmembrane protein kinase, putative similar to GI:3641252 from [Malus x domestica] (Plant Mol. Biol. 40 (6), 945-957 (1999)) Length = 1095 Score = 27.1 bits (57), Expect = 7.6 Identities = 11/34 (32%), Positives = 21/34 (61%) Frame = +2 Query: 380 KDVDGLNTINEGRVXVGDLSGXIPCTPAGCVELI 481 KD+ L+ ++ ++ V +L G IP + A C +L+ Sbjct: 313 KDIGKLSKLSSLQLHVNNLMGSIPVSLANCTKLV 346 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,489,089 Number of Sequences: 28952 Number of extensions: 161039 Number of successful extensions: 338 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 322 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 330 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 947539968 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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