Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20033
(729 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 31 0.17
SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe... 27 2.1
SPAC823.16c |mug179||WD repeat protein Mug179|Schizosaccharomyce... 27 2.1
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 2.7
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 27 3.6
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 26 4.8
SPAC1782.09c |clp1|flp1|Cdc14-related protein phosphatase Clp1/F... 26 4.8
SPAC824.03c |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.3
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 26 6.3
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 26 6.3
SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 25 8.4
SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomy... 25 8.4
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 31.1 bits (67), Expect = 0.17
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = +1
Query: 106 YDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRVEATSIRYLFTC 285
Y+ P ++S + +L S L LD++ K L+L FP++I + RV + F C
Sbjct: 153 YELPDPETKKSKIK--ALQSQLVRVNGELDDLQKHLTLSFPTLIAKSRVFFGQLMKHFYC 210
Query: 286 L 288
L
Sbjct: 211 L 211
>SPBC27B12.11c |||transcription factor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 738
Score = 27.5 bits (58), Expect = 2.1
Identities = 17/57 (29%), Positives = 25/57 (43%)
Frame = +3
Query: 489 VAEPTETPLRM*VVKRWSSPPRATCGTLTSAWRQPRRPMRSRKCRSDHVRCQHQRRC 659
V+ T PL V + P+AT G + R P+ KC+ D+ +C R C
Sbjct: 256 VSNNTTPPLS--TVNKIIKKPKATTGKVKK--RLPQAKRACAKCQKDNKKCDDARPC 308
>SPAC823.16c |mug179||WD repeat protein Mug179|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 335
Score = 27.5 bits (58), Expect = 2.1
Identities = 17/80 (21%), Positives = 42/80 (52%), Gaps = 3/80 (3%)
Frame = -1
Query: 681 IWNRQELRIVSDVDSVRGRFYTFAISLVFWAVSKPTSTSRTLLSVVNSISSRLTFVEVSQ 502
++N + + +++ +++ +G FA+ + A + PT+ L+ +++ + +T +
Sbjct: 109 VYNLKNMELINTLNTSKGNVIAFAVHENYVAYNSPTNPGDIYLASLDT-AIPVTLIHCHS 167
Query: 501 WALQLASCYP---LAAFASA 451
A+Q+ +P L A ASA
Sbjct: 168 SAVQVVDFHPRGHLIATASA 187
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.1 bits (57), Expect = 2.7
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +2
Query: 59 PRHSTTMARHIGRITITT-PSVLTFGKACWTHI 154
P H+TT R I +I I PS+LT G +C HI
Sbjct: 553 PVHATT--RFIAQIAILELPSILTTGYSCVMHI 583
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 26.6 bits (56), Expect = 3.6
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = +1
Query: 97 YHHYDPFSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRV 252
+ Y F +E +T S + NL E+++L NM+ EL KFP + GR+
Sbjct: 1484 FTQYRLFDVRGKERTSNTMSTY-NL-EEVEYLVNMVDELLNKFPDVNFTGRI 1533
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 26.2 bits (55), Expect = 4.8
Identities = 19/59 (32%), Positives = 35/59 (59%), Gaps = 5/59 (8%)
Frame = +1
Query: 193 DNMMKELSLKFPSII-NEGRVEA-TSI---RYLFTCLVTNRKTST*KRKMEC*WCRLTV 354
+N++KE++ K+ SII N+ ++++ TSI YL+ V K ++K+ W +TV
Sbjct: 6 NNLLKEVAAKYASIILNDAKIKSLTSINSAEYLYDSFVEISKLPL-EKKLPLKWKLITV 63
>SPAC1782.09c |clp1|flp1|Cdc14-related protein phosphatase
Clp1/Flp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 537
Score = 26.2 bits (55), Expect = 4.8
Identities = 30/131 (22%), Positives = 52/131 (39%), Gaps = 9/131 (6%)
Frame = +3
Query: 180 NATLGQHDEGAVVEVPQHYKRRTR--GGDKYQISIHLPGYEQKDINVKAKNGV---LMVQ 344
NAT G +P+ + R G + LP N K KN + +
Sbjct: 370 NATNGTSQSNISTPLPEPTPGQPRKVSGHNPPSARRLPSASSVKFNEKLKNASKQSIQNE 429
Query: 345 ANSAFNHYX--KIQNLPWDVNSEGSWVYEKDVLKITFPLKQK--QPEDSKRPVAEPTETP 512
++++ Y +IQN + + G+ V+++ Q +P + P + PT +P
Sbjct: 430 NKASYSSYEDSEIQNDD-ETRTVGTPTETISVVRLRRSSSQSNIEPNGVRSPTSSPTGSP 488
Query: 513 LRM*VVKRWSS 545
+R RWSS
Sbjct: 489 IRRTSGNRWSS 499
>SPAC824.03c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 25.8 bits (54), Expect = 6.3
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -1
Query: 702 HLYLIKLIWNRQELRIVSDVDSVR 631
H Y+ + +WN Q L I D+ SVR
Sbjct: 168 HSYMFQFVWNHQALAIFPDL-SVR 190
>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 851
Score = 25.8 bits (54), Expect = 6.3
Identities = 10/40 (25%), Positives = 21/40 (52%)
Frame = +1
Query: 133 ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRV 252
E + + +W+++ +MQ D ++L F I N G++
Sbjct: 104 EGSTNVNEVWNDITEDMQSQDFSTEDLKQLFLLIFNNGKL 143
>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 920
Score = 25.8 bits (54), Expect = 6.3
Identities = 19/89 (21%), Positives = 42/89 (47%), Gaps = 1/89 (1%)
Frame = -2
Query: 401 IHIPGKVLYFXVMIKSTVSLHHQHSIFRFHVD-VFLFVTRQVNRYLILVASTRPSFIMLG 225
+ +P + L F + ++T+ L H S + ++ ++ + R +++ S + +F
Sbjct: 776 LSVPEQELPF-TLEQATMDLRHVRSNYLYNEGYLYRLEDSSIRRRFVVLRSKQLNFYAEK 834
Query: 224 NFNDSSFIMLSKCCISLARLDQSECVSNM 138
LSK +S+ ++ SE VSN+
Sbjct: 835 GGQYLDTFQLSKTVVSIPMVNFSEAVSNL 863
>SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 8.4
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = +1
Query: 46 AAVSAAPQYYHGSSHWPYHHYDPFSP 123
A +A PQYY +P HY P P
Sbjct: 502 ATDAAKPQYYIPKDPYPVPHYYPQQP 527
>SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 25.4 bits (53), Expect = 8.4
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = -2
Query: 674 IGKNSASSLMLTAYVVASTLSRSHWSSGLSPSR 576
+ +NS++S M T+Y S S S SS S S+
Sbjct: 481 VSRNSSASTMSTSYTSGSGSSNSSGSSSNSSSK 513
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,049,455
Number of Sequences: 5004
Number of extensions: 63053
Number of successful extensions: 204
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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