Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbS20027
(677 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY686596-1|AAT96374.1| 1946|Apis mellifera Dscam protein. 33 0.002
AB269871-1|BAF03050.1| 1923|Apis mellifera cell adhesion molecul... 33 0.002
AB257298-1|BAE93381.1| 1919|Apis mellifera Dscam family member A... 33 0.002
AB231585-1|BAE17127.1| 898|Apis mellifera Mahya protein. 27 0.22
DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 23 2.0
AB252421-1|BAE80739.1| 122|Apis mellifera GB15078 protein. 23 2.7
AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 22 6.2
AY463910-1|AAR24352.1| 843|Apis mellifera metabotropic glutamat... 22 6.2
AB161181-1|BAD08343.1| 933|Apis mellifera metabotropic glutamat... 22 6.2
>AY686596-1|AAT96374.1| 1946|Apis mellifera Dscam protein.
Length = 1946
Score = 33.5 bits (73), Expect = 0.002
Identities = 24/63 (38%), Positives = 31/63 (49%)
Frame = +2
Query: 326 IRRKPKPTIQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGE 505
+R P T+ W KDG PL LE+ + LR+ KKE D+G+Y C RN
Sbjct: 331 VRGNPIKTVSWLKDGKPLG---------LEEAV--LRIESVKKE-DKGMYQCFVRNDQES 378
Query: 506 ALA 514
A A
Sbjct: 379 AQA 381
Score = 31.1 bits (67), Expect = 0.010
Identities = 20/70 (28%), Positives = 35/70 (50%), Gaps = 3/70 (4%)
Frame = +2
Query: 329 RRKPKPTIQWYK-DGTPLTSNDHPQRVLLEDGLLF--LRVMRGKKESDEGVYWCVARNPV 499
R P+P I W + DG+ + ++VL L+F R ++E VY C+AR+P
Sbjct: 28 RGNPQPDIIWVRADGSAVGDVPGLRQVLPNGNLVFPPFRAEDYRQEVHAQVYSCLARSPA 87
Query: 500 GEALAKTLRL 529
G ++ + +
Sbjct: 88 GSVHSRDVNV 97
Score = 29.9 bits (64), Expect = 0.023
Identities = 22/64 (34%), Positives = 29/64 (45%)
Frame = +2
Query: 338 PKPTIQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEALAK 517
P +I W +D L N Q+V L+ V R SD+ Y CVARN G +
Sbjct: 518 PIESIVWERDTRVLPIN-RKQKVFPNGTLIIENVER---MSDQATYTCVARNAQGYSARG 573
Query: 518 TLRL 529
TL +
Sbjct: 574 TLEV 577
Score = 29.5 bits (63), Expect = 0.031
Identities = 24/77 (31%), Positives = 37/77 (48%), Gaps = 7/77 (9%)
Frame = +2
Query: 338 PKPTIQWYK--DGT----PLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPV 499
P P +WYK +G+ P+ N+ ++V G L +R R + D G Y C+ N V
Sbjct: 240 PVPVHRWYKFIEGSSRRQPVQLNERVRQV---SGTLIIREARVE---DSGKYLCIVNNSV 293
Query: 500 -GEALAKTLRLLLPYSA 547
GE++ L + P A
Sbjct: 294 GGESVETVLTVTAPLGA 310
Score = 28.7 bits (61), Expect = 0.054
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
Frame = +2
Query: 338 PKPTIQWYKDGTPLTSNDH---PQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEA 508
P P I W DG L++ + Q V + ++ + +D G+Y C+A + VG A
Sbjct: 421 PTPEITWELDGKRLSNTERLQVGQYVTVNGDVVSHLNISSTHTNDGGLYKCIAASKVGSA 480
Score = 28.3 bits (60), Expect = 0.071
Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 6/68 (8%)
Frame = +2
Query: 338 PKPTIQWYKDG--TPLTSND----HPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPV 499
PKP + W K TP D +P + +EDG L + ++ +++EG Y C A N +
Sbjct: 706 PKPQVTWKKAAGDTPGDYTDLKLSNPD-ISVEDGTLSINNIQ---KTNEGYYLCEAVNGI 761
Query: 500 GEALAKTL 523
G L+ +
Sbjct: 762 GAGLSAVI 769
Score = 22.2 bits (45), Expect = 4.7
Identities = 13/50 (26%), Positives = 20/50 (40%)
Frame = +1
Query: 502 GSISKNATLTIAVLRDEFKIEPHDVKVASGEPALLQCASPKGVPDSVYTL 651
GS+ AV+ + + + G A+L+C P V D V L
Sbjct: 88 GSVHSRDVNVRAVVAQYYDTDVNKEYAIRGNSAILKCVVPSFVADFVKVL 137
Score = 22.2 bits (45), Expect = 4.7
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +1
Query: 553 FKIEPHDVKVASGEPALLQC 612
F+I+ + GEPA+LQC
Sbjct: 779 FEIKLKNQTARRGEPAVLQC 798
>AB269871-1|BAF03050.1| 1923|Apis mellifera cell adhesion molecule
AbsCAM-Ig7B protein.
Length = 1923
Score = 33.5 bits (73), Expect = 0.002
Identities = 26/77 (33%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 314 SAVCIRRK-PKPTIQWYKD--GTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCV 484
S VC+ + P P +WY P+ P+ LL L V D G+Y C
Sbjct: 256 SLVCVAQACPTPEYRWYAQTGSEPMLVLSGPRTRLLGSVLALEAVTL----EDNGIYRCS 311
Query: 485 ARNPVGEALAKTLRLLL 535
A NP GEA A+ +RL++
Sbjct: 312 ASNPGGEASAE-IRLIV 327
Score = 30.7 bits (66), Expect = 0.013
Identities = 21/61 (34%), Positives = 32/61 (52%)
Frame = +2
Query: 338 PKPTIQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEALAK 517
P I+W + L +D Q+VL DG L + ++ K+ D GVY C ARN G + +
Sbjct: 546 PIEEIKWERANRELP-DDLRQKVL-PDGTLVITSVQ--KKGDAGVYTCSARNKQGHSARR 601
Query: 518 T 520
+
Sbjct: 602 S 602
Score = 29.9 bits (64), Expect = 0.023
Identities = 23/71 (32%), Positives = 25/71 (35%), Gaps = 3/71 (4%)
Frame = +2
Query: 302 RTRYSAVCIRRKPKPTIQWYKDGT---PLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGV 472
RTR P TI W KDG PL N + D L + G
Sbjct: 627 RTRTVCGVAAGDPPLTISWLKDGQSPFPLPPNLASANISQLDPYSSLLSITNLAAEHSGD 686
Query: 473 YWCVARNPVGE 505
Y CVA NP E
Sbjct: 687 YTCVAANPAAE 697
Score = 29.1 bits (62), Expect = 0.041
Identities = 17/62 (27%), Positives = 24/62 (38%)
Frame = +2
Query: 350 IQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEALAKTLRL 529
I WYKDG L +L +G+ D G+Y C+ R G+ + L
Sbjct: 365 ITWYKDGRQLPGTGRQSELLRLNGI---------NREDRGMYQCIVRRSEGDTAQASAEL 415
Query: 530 LL 535
L
Sbjct: 416 QL 417
Score = 25.8 bits (54), Expect = 0.38
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +2
Query: 347 TIQWYKDG----TPLTS-NDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVG 502
T+ W K G P T+ +R + DG++ + + SD G Y+C A N G
Sbjct: 838 TVTWLKGGKIELNPSTNYRVTVKREVTPDGVIAQLQISSAEASDSGAYFCQASNLYG 894
>AB257298-1|BAE93381.1| 1919|Apis mellifera Dscam family member
AbsCAM-Ig7A protein.
Length = 1919
Score = 33.5 bits (73), Expect = 0.002
Identities = 26/77 (33%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 314 SAVCIRRK-PKPTIQWYKD--GTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCV 484
S VC+ + P P +WY P+ P+ LL L V D G+Y C
Sbjct: 256 SLVCVAQACPTPEYRWYAQTGSEPMLVLSGPRTRLLGSVLALEAVTL----EDNGIYRCS 311
Query: 485 ARNPVGEALAKTLRLLL 535
A NP GEA A+ +RL++
Sbjct: 312 ASNPGGEASAE-IRLIV 327
Score = 31.1 bits (67), Expect = 0.010
Identities = 30/114 (26%), Positives = 52/114 (45%), Gaps = 2/114 (1%)
Frame = +2
Query: 338 PKPTIQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEALAK 517
P I+W + L +D Q+VL DG L + ++ K+ D GVY C ARN G + +
Sbjct: 546 PIEEIKWERANRELP-DDLRQKVL-PDGTLVITSVQ--KKGDAGVYTCSARNKQGHSARR 601
Query: 518 T--LRLLLPYSAMNLRSNLTM*K*LVANLLFYNAHHRKGCLIPSIHWNKDGHSL 673
+ + +++P ++ + + +G L SI W KDG ++
Sbjct: 602 SGDVAVIVPPKISPFTADRDL---HLGERTTLTCSVTRGDLPLSISWLKDGRAM 652
Score = 29.1 bits (62), Expect = 0.041
Identities = 17/62 (27%), Positives = 24/62 (38%)
Frame = +2
Query: 350 IQWYKDGTPLTSNDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVGEALAKTLRL 529
I WYKDG L +L +G+ D G+Y C+ R G+ + L
Sbjct: 365 ITWYKDGRQLPGTGRQSELLRLNGI---------NREDRGMYQCIVRRSEGDTAQASAEL 415
Query: 530 LL 535
L
Sbjct: 416 QL 417
Score = 25.8 bits (54), Expect = 0.38
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +2
Query: 347 TIQWYKDG----TPLTS-NDHPQRVLLEDGLLFLRVMRGKKESDEGVYWCVARNPVG 502
T+ W K G P T+ +R + DG++ + + SD G Y+C A N G
Sbjct: 834 TVTWLKGGKIELNPSTNYRVTVKREVTPDGVIAQLQISSAEASDSGAYFCQASNLYG 890
>AB231585-1|BAE17127.1| 898|Apis mellifera Mahya protein.
Length = 898
Score = 26.6 bits (56), Expect = 0.22
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = +2
Query: 338 PKPTIQWYKDGTPLTSNDHPQRVLLEDGLLFL 433
P P + W ++G L + + P+ + DG L+L
Sbjct: 338 PPPPLVWRRNGADLETLNEPEIRVFNDGSLYL 369
Score = 26.6 bits (56), Expect = 0.22
Identities = 15/52 (28%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +2
Query: 335 KPKPTIQWYKDGTPLTSNDHPQRV-LLEDGLLFLRVMRGKKESDEGVYWCVA 487
+P P +QW K+ L ++D P + L+ +G +++ +D G Y C A
Sbjct: 428 EPLPRVQWLKNDEAL-NHDQPDKYDLIGNGTKL--IIKNVDYADTGAYMCQA 476
>DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine
receptor alpha3subunit protein.
Length = 566
Score = 23.4 bits (48), Expect = 2.0
Identities = 13/51 (25%), Positives = 25/51 (49%)
Frame = -3
Query: 471 TPSSLSFLPRITLRKSNPSSSSTLCGWSLEVRGVPSLYHCIVGLGFLRIHT 319
+P++ + P++ +S + +TL W P +Y I G+ F+ HT
Sbjct: 447 SPATTAAPPQLPTEESVDALCNTLHHW----HHCPEIYKAIEGIRFIADHT 493
Score = 21.4 bits (43), Expect = 8.2
Identities = 6/12 (50%), Positives = 10/12 (83%)
Frame = -3
Query: 666 CPSLFQCIDGIR 631
CP +++ I+GIR
Sbjct: 476 CPEIYKAIEGIR 487
>AB252421-1|BAE80739.1| 122|Apis mellifera GB15078 protein.
Length = 122
Score = 23.0 bits (47), Expect = 2.7
Identities = 8/14 (57%), Positives = 9/14 (64%)
Frame = +2
Query: 338 PKPTIQWYKDGTPL 379
P+P I W KDG L
Sbjct: 50 PRPEITWLKDGIEL 63
>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
protein.
Length = 1370
Score = 21.8 bits (44), Expect = 6.2
Identities = 14/40 (35%), Positives = 17/40 (42%)
Frame = +1
Query: 532 IAVLRDEFKIEPHDVKVASGEPALLQCASPKGVPDSVYTL 651
I L + +KI VK + SP VPDSV L
Sbjct: 583 IESLGNYYKIRDSKVKTLDASHNRITELSPLSVPDSVELL 622
>AY463910-1|AAR24352.1| 843|Apis mellifera metabotropic glutamate
receptor 1 protein.
Length = 843
Score = 21.8 bits (44), Expect = 6.2
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = -2
Query: 475 VDALVAFLLASHNPKEE 425
VDA+ AF LA HN +++
Sbjct: 334 VDAVYAFALALHNLQKD 350
>AB161181-1|BAD08343.1| 933|Apis mellifera metabotropic glutamate
receptor protein.
Length = 933
Score = 21.8 bits (44), Expect = 6.2
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = -2
Query: 475 VDALVAFLLASHNPKEE 425
VDA+ AF LA HN +++
Sbjct: 424 VDAVYAFALALHNLQKD 440
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 200,379
Number of Sequences: 438
Number of extensions: 4578
Number of successful extensions: 33
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20586735
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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