BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20026 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13220.2 68414.m01534 nuclear matrix constituent protein-rela... 31 0.67 At3g26420.1 68416.m03295 glycine-rich RNA-binding protein simila... 30 1.5 At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 29 3.6 At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 29 3.6 At1g40133.1 68414.m04768 hypothetical protein 29 3.6 At2g18810.1 68415.m02189 hypothetical protein contains Pfam prof... 28 6.2 At5g08780.1 68418.m01041 histone H1/H5 family protein contains P... 27 8.2 >At1g13220.2 68414.m01534 nuclear matrix constituent protein-related similar to nuclear matrix constituent protein 1 (NMCP1) [Daucus carota] GI:2190187 Length = 1128 Score = 31.1 bits (67), Expect = 0.67 Identities = 28/93 (30%), Positives = 44/93 (47%), Gaps = 1/93 (1%) Frame = +2 Query: 236 LTRKDSSVEEIQEKLKAAEERRRSLE-XXXXXXXXXXXXXXXXXSRIRSEQTNNFIVATK 412 L K +++E +EK+ AEE+R SLE +IR+E T + + Sbjct: 446 LEAKLKTIKE-REKIIQAEEKRLSLEKQQLLSDKESLEDLQQEIEKIRAEMTKKEEMIEE 504 Query: 413 EALDAKMETHEEKREAYINELRSRLKDHLEGVR 511 E +E +E+RE Y+ L+S LK +E R Sbjct: 505 EC--KSLEIKKEEREEYL-RLQSELKSQIEKSR 534 >At3g26420.1 68416.m03295 glycine-rich RNA-binding protein similar to RNA-binding protein (RZ-1) GB:BAA12064 [Nicotiana sylvestris]; contains Pfam profile: PF00076 RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) Length = 245 Score = 29.9 bits (64), Expect = 1.5 Identities = 14/33 (42%), Positives = 18/33 (54%) Frame = +1 Query: 406 HQGGARRQDGDPRGKTRGLHQRAALPSQGSS*G 504 HQGGA R + RG+ RG + + PS G G Sbjct: 86 HQGGAGRDNDGDRGRDRGYDRDRSRPSGGRGGG 118 >At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to IF2 protein [Drosophila melanogaster] GI:7108770; contains Pfam profile PF03144: Elongation factor Tu domain 2 Length = 1294 Score = 28.7 bits (61), Expect = 3.6 Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 3/75 (4%) Frame = +2 Query: 260 EEIQEKLKAAEERRRSLEXXXXXXXXXXXXXXXXXSRIRSEQTNNFIVATK---EALDAK 430 +E +E+ K EE + E R R E+ ++ K + L AK Sbjct: 424 QEAEERKKKEEEEKLRKEEEERRRQEELEAQAEEAKRKRKEKEKEKLLRKKLEGKLLTAK 483 Query: 431 METHEEKREAYINEL 475 +T +KREA+ N+L Sbjct: 484 QKTEAQKREAFKNQL 498 >At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03144: Elongation factor Tu domain 2 Length = 1201 Score = 28.7 bits (61), Expect = 3.6 Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 3/75 (4%) Frame = +2 Query: 260 EEIQEKLKAAEERRRSLEXXXXXXXXXXXXXXXXXSRIRSEQTNNFIVATK---EALDAK 430 +E +E+ K EE + E R R E+ ++ K + L AK Sbjct: 377 QEAEERKKKEEEEKLRKEEEERRRQEELEAQAEEAKRKRKEKEKEKLLRKKLEGKLLTAK 436 Query: 431 METHEEKREAYINEL 475 +T +KREA+ N+L Sbjct: 437 QKTEAQKREAFKNQL 451 >At1g40133.1 68414.m04768 hypothetical protein Length = 663 Score = 28.7 bits (61), Expect = 3.6 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +2 Query: 383 QTNNFIVATKEALDAKMETHEEKREAYINE 472 + NN A +A AKME E +REA++N+ Sbjct: 474 EANNRDEALSQAAAAKMEKEEVEREAFVNK 503 >At2g18810.1 68415.m02189 hypothetical protein contains Pfam profile PF03754: Domain of unknown function (DUF313) Length = 234 Score = 27.9 bits (59), Expect = 6.2 Identities = 13/31 (41%), Positives = 17/31 (54%) Frame = -2 Query: 562 RWPCTLPRSAVPGSTWSSNALKMILETGAQL 470 RW T + V GS W+ + ILETG +L Sbjct: 136 RWDMTSTSNYVLGSGWNKVVTENILETGTRL 166 >At5g08780.1 68418.m01041 histone H1/H5 family protein contains Pfam domain, PF00538: linker histone H1 and H5 family Length = 457 Score = 27.5 bits (58), Expect = 8.2 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 111 VEAMEVETKSTEIRCQEMSKGGLAYEV 191 VE ++VE E R + S+GG YEV Sbjct: 229 VEVVDVENSENEARIEANSRGGELYEV 255 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,439,798 Number of Sequences: 28952 Number of extensions: 174179 Number of successful extensions: 653 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 634 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 653 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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