BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20018 (606 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 174 1e-44 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 97 1e-21 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 95 7e-21 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 92 5e-20 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 59 4e-10 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 58 8e-10 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 48 8e-07 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 33 0.043 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.30 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 30 0.30 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.8 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.7 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 4.9 SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces pom... 25 8.6 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 174 bits (423), Expect = 1e-44 Identities = 78/91 (85%), Positives = 82/91 (90%) Frame = +1 Query: 238 GTEGLFVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 417 G + +VGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE Sbjct: 48 GQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTE 107 Query: 418 APLNPKANREXMTQIMFXTFNTPAMYVAIQA 510 APLNPK+NRE MTQI+F TFN PA YVAIQA Sbjct: 108 APLNPKSNREKMTQIIFETFNAPAFYVAIQA 138 Score = 106 bits (254), Expect = 3e-24 Identities = 46/51 (90%), Positives = 50/51 (98%) Frame = +2 Query: 101 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDS 253 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDS Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDS 52 Score = 66.5 bits (155), Expect = 3e-12 Identities = 30/33 (90%), Positives = 32/33 (96%) Frame = +3 Query: 507 SVLSLYASGRXTGIVLDSGDGVSHTVPIYEGYA 605 +VLSLYASGR TGIVLDSGDGV+HTVPIYEGYA Sbjct: 138 AVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYA 170 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 97.5 bits (232), Expect = 1e-21 Identities = 40/96 (41%), Positives = 66/96 (68%) Frame = +1 Query: 223 RDGRYGTEGLFVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 402 + G + + VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 403 VLLTEAPLNPKANREXMTQIMFXTFNTPAMYVAIQA 510 +LLTE P+NP ANRE M + MF + +YVAIQA Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQA 138 Score = 49.2 bits (112), Expect = 5e-07 Identities = 20/30 (66%), Positives = 26/30 (86%) Frame = +3 Query: 507 SVLSLYASGRXTGIVLDSGDGVSHTVPIYE 596 +VLSLYA G +G+V+DSGDGV+H VP+YE Sbjct: 138 AVLSLYAQGLSSGVVVDSGDGVTHIVPVYE 167 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +2 Query: 107 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 211 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 95.1 bits (226), Expect = 7e-21 Identities = 41/87 (47%), Positives = 55/87 (63%), Gaps = 1/87 (1%) Frame = +1 Query: 253 FVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLN 429 F+G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLN Sbjct: 75 FIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLN 134 Query: 430 PKANREXMTQIMFXTFNTPAMYVAIQA 510 P NRE +IMF +FN +Y+A+QA Sbjct: 135 PPENRENTAEIMFESFNCAGLYIAVQA 161 Score = 40.7 bits (91), Expect = 2e-04 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +2 Query: 119 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 202 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 Score = 37.9 bits (84), Expect = 0.001 Identities = 14/21 (66%), Positives = 18/21 (85%) Frame = +3 Query: 540 TGIVLDSGDGVSHTVPIYEGY 602 TG V+DSGDGV+H +P+ EGY Sbjct: 180 TGTVVDSGDGVTHIIPVAEGY 200 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 92.3 bits (219), Expect = 5e-20 Identities = 40/87 (45%), Positives = 59/87 (67%), Gaps = 1/87 (1%) Frame = +1 Query: 250 LFVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPL 426 +FVG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE PL Sbjct: 56 MFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115 Query: 427 NPKANREXMTQIMFXTFNTPAMYVAIQ 507 N+E + + + T N PA+ ++Q Sbjct: 116 ANIRNKEKIAEYFYETLNVPALSFSLQ 142 Score = 58.8 bits (136), Expect = 6e-10 Identities = 25/44 (56%), Positives = 32/44 (72%) Frame = +2 Query: 119 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKD 250 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKD 55 Score = 52.0 bits (119), Expect = 7e-08 Identities = 21/32 (65%), Positives = 29/32 (90%) Frame = +3 Query: 510 VLSLYASGRXTGIVLDSGDGVSHTVPIYEGYA 605 VL+LYAS R TGIVL+ GDG++H+VPIY+G++ Sbjct: 144 VLALYASARTTGIVLECGDGLTHSVPIYDGFS 175 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 59.3 bits (137), Expect = 4e-10 Identities = 29/95 (30%), Positives = 52/95 (54%), Gaps = 1/95 (1%) Frame = +1 Query: 223 RDGRYGTEGLFVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 399 RD + VG++ + G ++ + P E +++NWD ME++ +TF +L + EH Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116 Query: 400 PVLLTEAPLNPKANREXMTQIMFXTFNTPAMYVAI 504 P+ +TE NP R MT+++F +N P++ I Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSVAYGI 151 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +2 Query: 119 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 214 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 Score = 26.2 bits (55), Expect = 3.7 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = +3 Query: 540 TGIVLDSGDGVSHTVPIYEG 599 +GIVL+ G+ SH +P+ G Sbjct: 166 SGIVLNLGNAASHVIPVLNG 185 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 58.4 bits (135), Expect = 8e-10 Identities = 27/73 (36%), Positives = 39/73 (53%) Frame = +1 Query: 289 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREXMTQIMF 468 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 469 XTFNTPAMYVAIQ 507 + PA Y+A Q Sbjct: 123 ESLRCPATYLAKQ 135 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 104 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 205 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 48.4 bits (110), Expect = 8e-07 Identities = 25/88 (28%), Positives = 45/88 (51%), Gaps = 2/88 (2%) Frame = +1 Query: 253 FVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL 426 +V DE Q I + +K +GI+ +W+ W +L+V P E+ +++TE Sbjct: 52 YVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSW 111 Query: 427 NPKANREXMTQIMFXTFNTPAMYVAIQA 510 NP++ R+ + + F + PA Y+ QA Sbjct: 112 NPQSVRQQIMEAAFEQLHVPAFYLTKQA 139 Score = 36.7 bits (81), Expect = 0.003 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +2 Query: 104 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 205 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 32.7 bits (71), Expect = 0.043 Identities = 20/72 (27%), Positives = 31/72 (43%), Gaps = 4/72 (5%) Frame = +1 Query: 304 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREXMTQIMFX 471 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ F Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 472 TFNTPAMYVAIQ 507 P +A + Sbjct: 172 ECQVPGFTIAYE 183 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 29.9 bits (64), Expect = 0.30 Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 5/53 (9%) Frame = +1 Query: 364 FYNELRVAPEEHPVLLTE--APLNPKANREXMTQIMFXTFNTPAM---YVAIQ 507 +Y L E+HP+LLT+ A L P+ + + +I + NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 29.9 bits (64), Expect = 0.30 Identities = 19/66 (28%), Positives = 29/66 (43%) Frame = -1 Query: 336 IPVSDDSVFDGVFEGQDTSFALCLISYKESFCPIPTITP*WRGLPTIEGNTARGASSPAK 157 +P + +V +TSFAL S S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 156 PALHIP 139 P L+ P Sbjct: 123 PQLNSP 128 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 2.8 Identities = 15/45 (33%), Positives = 18/45 (40%) Frame = -1 Query: 234 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 100 PT+ P P N S P PL+TTN TS+S Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 3.7 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -1 Query: 240 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 148 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 25.8 bits (54), Expect = 4.9 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = -2 Query: 257 TKSPSVPYRPSRPDGGAFPRSRGTR 183 +K+PS PY SRP A S TR Sbjct: 311 SKAPSYPYSSSRPSASASLASSPTR 335 >SPBC16H5.12c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 682 Score = 25.0 bits (52), Expect = 8.6 Identities = 9/26 (34%), Positives = 13/26 (50%) Frame = -3 Query: 562 PESSTIPVXRPDAYSESTLGWRRTSR 485 PE + + R D+Y GW R +R Sbjct: 611 PEGVRVNIMRDDSYYRQNRGWYRNNR 636 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,480,665 Number of Sequences: 5004 Number of extensions: 51339 Number of successful extensions: 163 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 266270664 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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