BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20012 (652 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 148 8e-37 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 28 1.0 SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr... 27 1.8 SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 27 2.3 SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit Gsa1... 27 2.3 SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 27 2.3 SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces ... 25 9.5 SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 25 9.5 SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 25 9.5 SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 25 9.5 >SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 418 Score = 148 bits (358), Expect = 8e-37 Identities = 69/104 (66%), Positives = 80/104 (76%) Frame = +2 Query: 257 DMVAQAIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436 DMVAQAIK +GGFVWACKNYDGDV SD+VAQ YGSLG+MTSVL+ P+GRT ESE+AHGTV Sbjct: 263 DMVAQAIKSNGGFVWACKNYDGDVMSDVVAQAYGSLGLMTSVLIHPNGRTFESEAAHGTV 322 Query: 437 TRHYRMHQQGKPTSTNPVASIYAWQEVLHTGPN*DGTPELERFA 568 RHY + +GK TSTN +ASI+AW L DG L +FA Sbjct: 323 QRHYMQYLKGKKTSTNSIASIFAWTRGLAHRGRLDGNERLVKFA 366 Score = 140 bits (339), Expect = 2e-34 Identities = 61/82 (74%), Positives = 72/82 (87%) Frame = +3 Query: 9 LYDFKTPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEE 188 +Y+F GVAM MYNTD+SIR FAHSSFQ+ALQKK PLYLSTKNTILK+YDGRFKD F+E Sbjct: 180 VYEFNGSGVAMSMYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTILKKYDGRFKDTFQE 239 Query: 189 VFQSDYKTKFDEAKIWYEHRLI 254 V++SDYK KF+E +WY+HRLI Sbjct: 240 VYESDYKQKFEELGLWYQHRLI 261 Score = 32.7 bits (71), Expect = 0.047 Identities = 16/42 (38%), Positives = 24/42 (57%) Frame = +1 Query: 511 RGLAHRAKLGRDSRIGALRS*PSKRRXVECIDSGKMTKDLVI 636 RGLAHR +L + R+ + + V C++ G MTKDL + Sbjct: 348 RGLAHRGRLDGNERLVKFAN-ALEHACVRCVEKGIMTKDLYL 388 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 28.3 bits (60), Expect = 1.0 Identities = 11/30 (36%), Positives = 20/30 (66%) Frame = +3 Query: 132 TKNTILKRYDGRFKDIFEEVFQSDYKTKFD 221 T+ T+L DG+FKDIF++ D + +++ Sbjct: 414 TQETLLGLLDGKFKDIFDKCIIIDCRFEYE 443 >SPAC11D3.13 |||ThiJ domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 222 Score = 27.5 bits (58), Expect = 1.8 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +2 Query: 107 EKMASIPVYEEYHFETLRWSFQ 172 ++M +P +E+YHF+TL FQ Sbjct: 156 QQMNLMPTFEKYHFKTLNKLFQ 177 >SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr 1|||Manual Length = 621 Score = 27.1 bits (57), Expect = 2.3 Identities = 16/34 (47%), Positives = 19/34 (55%) Frame = -1 Query: 103 KAT*KLEWAKDRIDSSVLYMPIATPGVLKSYNTR 2 KAT K EW K++ID L I T LK+Y R Sbjct: 300 KATEKREWVKEKIDLESLLGTIQTN--LKTYQLR 331 >SPAC3F10.04 |gsa1|gsh2|glutathione synthetase large subunit Gsa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 27.1 bits (57), Expect = 2.3 Identities = 12/44 (27%), Positives = 22/44 (50%) Frame = +3 Query: 102 LQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKI 233 + K W LY + + + +F+ + VF+SDY D++ I Sbjct: 97 MNKLWNLYQKHREAVAHLKENQFQPLSLGVFRSDYMVHQDDSFI 140 >SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 603 Score = 27.1 bits (57), Expect = 2.3 Identities = 11/42 (26%), Positives = 21/42 (50%) Frame = +2 Query: 311 NYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436 N+ G + + +QG+ + + S LMCP + E H ++ Sbjct: 244 NFVGKPAALVFSQGFSTNATVFSTLMCPGSLIISDELNHTSI 285 >SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 314 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = +2 Query: 284 SGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436 S G+ A K +D + S + YG G +++V + PDG S GT+ Sbjct: 167 SAGWDKAVKVWDLETFS-LRTSHYGHTGYVSAVTISPDGSLCASGGRDGTL 216 >SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 362 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/41 (39%), Positives = 21/41 (51%) Frame = +2 Query: 311 NYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGT 433 N GD+ SD A GSLG++ S + V SE HG+ Sbjct: 252 NLYGDILSDGAASLIGSLGLVPSANV--GDNFVMSEPVHGS 290 >SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces pombe|chr 1|||Manual Length = 567 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/35 (28%), Positives = 17/35 (48%) Frame = -3 Query: 440 VSPSRAPIRIPRYGHRDTSILMSSFPMTRNPEQQY 336 +SPS P +P Y H ++ + +P P + Y Sbjct: 421 LSPSSIPSGLPTYAHVNSPLGTEQYPSEAYPPKGY 455 >SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr 3|||Manual Length = 625 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/32 (37%), Positives = 16/32 (50%) Frame = +2 Query: 398 GRTVESESAHGTVTRHYRMHQQGKPTSTNPVA 493 G T S S+H + +H G TST+ VA Sbjct: 552 GSTSSSGSSHTITSTSQSVHTSGSSTSTSSVA 583 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,916,686 Number of Sequences: 5004 Number of extensions: 62412 Number of successful extensions: 172 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 171 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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