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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20012
         (652 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_26442| Best HMM Match : Iso_dh (HMM E-Value=3.6e-37)               153   9e-38
SB_17538| Best HMM Match : Iso_dh (HMM E-Value=0)                     121   6e-28
SB_1787| Best HMM Match : Peptidase_S9 (HMM E-Value=1.5e-08)           31   0.62 
SB_35142| Best HMM Match : Peptidase_M1 (HMM E-Value=2e-13)            30   1.4  
SB_12581| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   3.3  
SB_51024| Best HMM Match : 7tm_1 (HMM E-Value=5.8e-08)                 29   4.3  
SB_9471| Best HMM Match : VQ (HMM E-Value=6.2)                         28   5.7  
SB_57353| Best HMM Match : TIP49 (HMM E-Value=0)                       28   7.6  

>SB_26442| Best HMM Match : Iso_dh (HMM E-Value=3.6e-37)
          Length = 296

 Score =  153 bits (372), Expect = 9e-38
 Identities = 68/79 (86%), Positives = 74/79 (93%)
 Frame = +2

Query: 257 DMVAQAIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           DMVA A+K  GGF+WACKNYDGDVQSD VAQG+GSLGMMTSVL+CPDG+TVESE+AHGTV
Sbjct: 194 DMVAFALKTEGGFIWACKNYDGDVQSDSVAQGFGSLGMMTSVLVCPDGKTVESEAAHGTV 253

Query: 437 TRHYRMHQQGKPTSTNPVA 493
           TRHYRMHQQGK TSTNPVA
Sbjct: 254 TRHYRMHQQGKETSTNPVA 272



 Score = 68.5 bits (160), Expect = 4e-12
 Identities = 38/75 (50%), Positives = 46/75 (61%)
 Frame = +3

Query: 30  GVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYK 209
           GV MGM+NTD SIR+FAHSSFQ AL KK+PLY+                        +YK
Sbjct: 141 GVTMGMFNTDVSIRAFAHSSFQYALDKKYPLYM-----------------------REYK 177

Query: 210 TKFDEAKIWYEHRLI 254
           +KF+E+ IWYEHRLI
Sbjct: 178 SKFEESNIWYEHRLI 192


>SB_17538| Best HMM Match : Iso_dh (HMM E-Value=0)
          Length = 644

 Score =  121 bits (291), Expect = 6e-28
 Identities = 60/103 (58%), Positives = 71/103 (68%)
 Frame = +2

Query: 257 DMVAQAIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           DMVA A+K  GGFVWA KNYDGDVQSD +AQ               DG+T+ESE+AHGTV
Sbjct: 242 DMVAYALKSEGGFVWAAKNYDGDVQSDTLAQ---------------DGKTIESEAAHGTV 286

Query: 437 TRHYRMHQQGKPTSTNPVASIYAWQEVLHTGPN*DGTPELERF 565
           TRHYR HQ+G  TSTNP+ASI+AW + L      DG PEL++F
Sbjct: 287 TRHYREHQKGNETSTNPIASIFAWTQGLSHRAKLDGNPELKKF 329



 Score =  120 bits (290), Expect = 8e-28
 Identities = 51/77 (66%), Positives = 65/77 (84%)
 Frame = +3

Query: 24  TPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSD 203
           T GV MGMYNTDE+IR FAHS  Q A+ K+ PLY+STKNTILK+YDGRFKDIF+++++ +
Sbjct: 164 TGGVMMGMYNTDEAIRDFAHSCMQYAIHKQVPLYMSTKNTILKKYDGRFKDIFQDIYERE 223

Query: 204 YKTKFDEAKIWYEHRLI 254
           Y+++F E  IWYEHRLI
Sbjct: 224 YESQFKELGIWYEHRLI 240



 Score = 41.1 bits (92), Expect = 8e-04
 Identities = 20/47 (42%), Positives = 29/47 (61%)
 Frame = +1

Query: 511 RGLAHRAKLGRDSRIGALRS*PSKRRXVECIDSGKMTKDLVICIHGL 651
           +GL+HRAKL  +  +        ++  V+ +D GKMTKDL  CI+GL
Sbjct: 312 QGLSHRAKLDGNPELKKFCK-ALEKACVDTVDQGKMTKDLAACIYGL 357


>SB_1787| Best HMM Match : Peptidase_S9 (HMM E-Value=1.5e-08)
          Length = 1057

 Score = 31.5 bits (68), Expect = 0.62
 Identities = 30/92 (32%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
 Frame = +2

Query: 350 GYGSLGMMTSVLMCPDGRTVESESAHGTVT-RHYRMHQQGKPTSTNPVASIYAWQEVLHT 526
           G+G+L   T +  C D  T +S   HGTVT R    H+ G  T T  +   Y    +   
Sbjct: 44  GHGTL--TTRLRSCHDTITAQSHHNHGTVTPRSRHTHRDGHGTLTTRLR--YCHDTITAQ 99

Query: 527 GPN*DGTPELERFALSPRRGVXSSASIAVK*Q 622
             +  GT    R   SPRR   +  +IAV+ Q
Sbjct: 100 SHHNHGT-VTPRSRHSPRRSRYTHDAIAVQSQ 130


>SB_35142| Best HMM Match : Peptidase_M1 (HMM E-Value=2e-13)
          Length = 302

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 14/48 (29%), Positives = 23/48 (47%)
 Frame = +3

Query: 93  QVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIW 236
           Q+ L+ K  L    K TI   + G   +     ++S YKT+  E ++W
Sbjct: 167 QLLLEPKQGLVKGQKFTIKLEFSGELSNKMAGFYKSSYKTRNGEVRVW 214


>SB_12581| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 756

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +1

Query: 295 CLGLQKLRRRCAVRYCCSGLRVIGNDDISIDVSRWPYR 408
           C G  K  R+C +  C +  RV+   D++ D++R  Y+
Sbjct: 127 CWGDSKKTRKCGIAPCPANKRVVNFLDVTFDLNRNTYQ 164


>SB_51024| Best HMM Match : 7tm_1 (HMM E-Value=5.8e-08)
          Length = 342

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 9/16 (56%), Positives = 13/16 (81%)
 Frame = +3

Query: 387 CVPMAVPWNPNRRTGR 434
           CVP+A+PW P+R + R
Sbjct: 296 CVPVALPWRPSRPSSR 311


>SB_9471| Best HMM Match : VQ (HMM E-Value=6.2)
          Length = 307

 Score = 28.3 bits (60), Expect = 5.7
 Identities = 15/42 (35%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
 Frame = +2

Query: 362 LGMMTSVLMCPDGRTVESESAHGTVTR-HYRMHQQGKPTSTN 484
           L    ++  CPDGR   SE + GTV++ H   H     T  N
Sbjct: 255 LDQAEAITACPDGRLFYSEKSSGTVSQVHAEGHDSVVLTGLN 296


>SB_57353| Best HMM Match : TIP49 (HMM E-Value=0)
          Length = 283

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 11/34 (32%), Positives = 18/34 (52%)
 Frame = +2

Query: 344 AQGYGSLGMMTSVLMCPDGRTVESESAHGTVTRH 445
           A+ Y ++G  T  + CP+G   + +    TVT H
Sbjct: 23  ARDYDAMGPQTKFVQCPEGELQKRKEVVHTVTLH 56


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,631,074
Number of Sequences: 59808
Number of extensions: 511411
Number of successful extensions: 1340
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1252
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1337
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1657237625
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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