Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbS20012
         (652 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA...   155   3e-38
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA...   150   7e-37
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA...   147   6e-36
At3g30848.1 68416.m03974 hypothetical protein contains similarit...    30   1.5  
At4g15480.1 68417.m02366 UDP-glucoronosyl/UDP-glucosyl transfera...    28   4.7  
At3g01085.1 68416.m00012 protein kinase family protein contains ...    28   4.7  
At2g04280.1 68415.m00420 expressed protein                             28   4.7  
At1g30730.1 68414.m03756 FAD-binding domain-containing protein s...    28   4.7  
At5g41970.1 68418.m05110 expressed protein contains Pfam profile...    27   8.2  
At4g32420.1 68417.m04615 peptidyl-prolyl cis-trans isomerase cyc...    27   8.2  
At4g12700.1 68417.m01994 expressed protein                             27   8.2  
At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NA...    27   8.2  
At2g39330.1 68415.m04828 jacalin lectin family protein similar t...    27   8.2  

>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
           limon]; Nicotiana tabacum SP|P50218
          Length = 416

 Score =  155 bits (375), Expect = 3e-38
 Identities = 66/88 (75%), Positives = 78/88 (88%)
 Frame = +2

Query: 257 DMVAQAIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           DMVA A+K  GG+VWACKNYDGDVQSD +AQGYGSLGMMTSVL+CPDG+T+E+E+AHGTV
Sbjct: 254 DMVAYAMKSEGGYVWACKNYDGDVQSDFLAQGYGSLGMMTSVLVCPDGKTIEAEAAHGTV 313

Query: 437 TRHYRMHQQGKPTSTNPVASIYAWQEVL 520
           TRHYR+HQ+G  TSTN +ASI+AW   L
Sbjct: 314 TRHYRVHQKGGETSTNSIASIFAWSRGL 341



 Score =  127 bits (306), Expect = 7e-30
 Identities = 55/82 (67%), Positives = 70/82 (85%)
 Frame = +3

Query: 9   LYDFKTPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEE 188
           +++F   GVA+ MYNTDESIR+FA SS   A QKKWPLYLSTKNTILK YDGRFKDIF+E
Sbjct: 171 VFNFTGGGVALAMYNTDESIRAFAESSMYTAYQKKWPLYLSTKNTILKIYDGRFKDIFQE 230

Query: 189 VFQSDYKTKFDEAKIWYEHRLI 254
           V+++++++K++ A IWYEHRLI
Sbjct: 231 VYEANWRSKYEAAGIWYEHRLI 252



 Score = 39.5 bits (88), Expect = 0.002
 Identities = 20/47 (42%), Positives = 30/47 (63%)
 Frame = +1

Query: 508 ARGLAHRAKLGRDSRIGALRS*PSKRRXVECIDSGKMTKDLVICIHG 648
           +RGLAHRAKL  ++ + +      +   +  ++SGKMTKDL + IHG
Sbjct: 338 SRGLAHRAKLDSNAALLSYTE-KLEAACMGTVESGKMTKDLALLIHG 383


>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase SP|Q40345 from [Medicago
           sativa]
          Length = 410

 Score =  150 bits (364), Expect = 7e-37
 Identities = 62/84 (73%), Positives = 77/84 (91%)
 Frame = +2

Query: 257 DMVAQAIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           DMVA A+K  GG+VWACKNYDGDVQSD +AQG+GSLG+MTSVL+CPDG+T+E+E+AHGTV
Sbjct: 253 DMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTV 312

Query: 437 TRHYRMHQQGKPTSTNPVASIYAW 508
           TRH+R+HQ+G  TSTN +ASI+AW
Sbjct: 313 TRHFRVHQKGGETSTNSIASIFAW 336



 Score =  128 bits (308), Expect = 4e-30
 Identities = 55/75 (73%), Positives = 66/75 (88%)
 Frame = +3

Query: 30  GVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYK 209
           GVAM MYNTDESIR+FA +S   A +KKWPLYLSTKNTILK+YDGRFKDIF+EV+++ +K
Sbjct: 177 GVAMAMYNTDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWK 236

Query: 210 TKFDEAKIWYEHRLI 254
           +K+D A IWYEHRLI
Sbjct: 237 SKYDAAGIWYEHRLI 251



 Score = 40.3 bits (90), Expect = 0.001
 Identities = 21/46 (45%), Positives = 29/46 (63%)
 Frame = +1

Query: 511 RGLAHRAKLGRDSRIGALRS*PSKRRXVECIDSGKMTKDLVICIHG 648
           RGLAHRAKL  ++++        +   V  ++SGKMTKDL + IHG
Sbjct: 338 RGLAHRAKLDDNAKLLDFTE-KLEAACVGTVESGKMTKDLALIIHG 382


>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
           GI:3021512; contains Pfam domain PF00180: dehydrogenase,
           isocitrate/isopropylmalate family
          Length = 485

 Score =  147 bits (356), Expect = 6e-36
 Identities = 65/103 (63%), Positives = 82/103 (79%)
 Frame = +2

Query: 257 DMVAQAIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTV 436
           DMVA A+K  GG+VWACKNYDGDVQSD++AQG+GSLG+MTSVL+  DG+T+ESE+AHGTV
Sbjct: 324 DMVAYAVKSEGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLLSADGKTLESEAAHGTV 383

Query: 437 TRHYRMHQQGKPTSTNPVASIYAWQEVLHTGPN*DGTPELERF 565
           TRH+R+HQ+G+ TSTN +ASI+AW   L      D   +L  F
Sbjct: 384 TRHFRLHQKGQETSTNSIASIFAWTRGLEHRAKLDKNEKLMDF 426



 Score =  139 bits (336), Expect = 2e-33
 Identities = 60/82 (73%), Positives = 72/82 (87%)
 Frame = +3

Query: 9   LYDFKTPGVAMGMYNTDESIRSFAHSSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEE 188
           +YDFK PGVA+ MYN DESIR+FA SS  +AL KKWPLYLSTKNTILK+YDGRFKDIF+E
Sbjct: 241 VYDFKGPGVALAMYNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQE 300

Query: 189 VFQSDYKTKFDEAKIWYEHRLI 254
           V+++++K KF+E  IWYEHRLI
Sbjct: 301 VYEANWKQKFEEHSIWYEHRLI 322



 Score = 38.7 bits (86), Expect = 0.003
 Identities = 19/46 (41%), Positives = 28/46 (60%)
 Frame = +1

Query: 511 RGLAHRAKLGRDSRIGALRS*PSKRRXVECIDSGKMTKDLVICIHG 648
           RGL HRAKL ++ ++        +   V  +++GKMTKDL + IHG
Sbjct: 409 RGLEHRAKLDKNEKLMDFVK-KLESSCVNTVETGKMTKDLALLIHG 453


>At3g30848.1 68416.m03974 hypothetical protein contains similarity
           to hypothetical proteins
          Length = 156

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = +3

Query: 129 STKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWYEHR 248
           STK     + D    ++  ++F +DYKTKF     W E R
Sbjct: 49  STKEKTSGQNDNYVVNLAHQIFYTDYKTKFSLEHAWREVR 88


>At4g15480.1 68417.m02366 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 490

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 1/31 (3%)
 Frame = -1

Query: 106 CKAT*K-LEWAKDRIDSSVLYMPIATPGVLK 17
           CK+T K LEW   R  SSV+Y+   T   LK
Sbjct: 271 CKSTDKCLEWLDSRPKSSVVYISFGTVAYLK 301


>At3g01085.1 68416.m00012 protein kinase family protein contains
           Pfam domain, PF00069: Protein kinase domain; contains
           non-consensus splice site (GC) at intron 2
          Length = 629

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
 Frame = +2

Query: 224 SQDMVRASFNYDMVAQAIKGSGGFVWA--CKNYDGDVQSDIVAQGYGSLGMMTSVLM 388
           SQ   R+ F Y+ ++Q    + GF WA   K  + DV S +     GS   ++ + M
Sbjct: 488 SQATTRSEFPYNSLSQTTAPASGFAWAGTKKRKENDVASTLTYIQPGSASHVSGMSM 544


>At2g04280.1 68415.m00420 expressed protein
          Length = 568

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 16/52 (30%), Positives = 27/52 (51%)
 Frame = +3

Query: 84  SSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWY 239
           S+ Q  +++   LY++T    L  ++   KD +   F  DYK  +DE+  WY
Sbjct: 462 STLQDKVEEGRHLYIATNEGELSFFNP-LKDKYATHFLYDYKDLWDESSEWY 512


>At1g30730.1 68414.m03756 FAD-binding domain-containing protein
           similar to SP|P30986 reticuline oxidase precursor
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase) [Eschscholzia
           californica]; contains PF01565 FAD binding domain
          Length = 526

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 14/29 (48%), Positives = 16/29 (55%)
 Frame = +3

Query: 168 FKDIFEEVFQSDYKTKFDEAKIWYEHRLI 254
           FKD FE +   D KTKFDE   W   + I
Sbjct: 496 FKDNFERLV--DIKTKFDEINFWRNEQSI 522


>At5g41970.1 68418.m05110 expressed protein contains Pfam profile
           PF03690: Uncharacterised protein family (UPF0160)
          Length = 346

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/31 (45%), Positives = 17/31 (54%)
 Frame = +3

Query: 153 RYDGRFKDIFEEVFQSDYKTKFDEAKIWYEH 245
           RYD   K  FEEVF   + TK   A + Y+H
Sbjct: 83  RYDHHQKG-FEEVFGHGFNTKLSSAGLVYKH 112


>At4g32420.1 68417.m04615 peptidyl-prolyl cis-trans isomerase
           cyclophilin-type family protein weak similarity to
           CARS-Cyp [Homo sapiens]  GI:1117968; contains Pfam
           profile PF00160: peptidyl-prolyl cis-trans isomerase,
           cyclophilin-type
          Length = 837

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 15/49 (30%), Positives = 26/49 (53%)
 Frame = -3

Query: 437 SPSRAPIRIPRYGHRDTSILMSSFPMTRNPEQQYRTAHLRRSFCRPKQS 291
           SPSR+P+R  R     + I +S   ++R+P +  R +  R     P++S
Sbjct: 522 SPSRSPVRSSRRSLSRSPIQLSRRSLSRSPTRLSRRSLSRSPIRSPRKS 570


>At4g12700.1 68417.m01994 expressed protein
          Length = 561

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/52 (26%), Positives = 28/52 (53%)
 Frame = +3

Query: 84  SSFQVALQKKWPLYLSTKNTILKRYDGRFKDIFEEVFQSDYKTKFDEAKIWY 239
           S+ Q  +++   LY++T    L  ++   KD ++  F  ++K  +DE+  WY
Sbjct: 455 STLQDKIEQGRNLYIATNEPELSFFNP-LKDKYKPHFLDEFKDLWDESSEWY 505


>At3g09810.1 68416.m01169 isocitrate dehydrogenase, putative / NAD+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NAD+) GB:CAA65502 GI:3021506
           [Nicotiana tabacum]
          Length = 374

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +2

Query: 311 NYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGT 433
           N  GD+ SD+ A   G LG+  S+ +  DG  + +E+ HG+
Sbjct: 265 NLYGDIISDLCAGLVGGLGLTPSMNIGEDGIAL-AEAVHGS 304


>At2g39330.1 68415.m04828 jacalin lectin family protein similar to
           myrosinase-binding protein homolog [Arabidopsis
           thaliana] GI:2997767, myrosinase binding protein
           [Brassica napus] GI:1711296; contains Pfam profile
           PF01419 jacalin-like lectin domain
          Length = 459

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 19/56 (33%), Positives = 26/56 (46%)
 Frame = +2

Query: 272 AIKGSGGFVWACKNYDGDVQSDIVAQGYGSLGMMTSVLMCPDGRTVESESAHGTVT 439
           AI GSGG  W    YDG V+  +V QG   +  +        G+ + S   HG +T
Sbjct: 317 AIGGSGGVAWDDGVYDG-VRKILVGQGNDGVAFVK--FEYNKGKDLVSGDDHGKMT 369


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,440,561
Number of Sequences: 28952
Number of extensions: 340203
Number of successful extensions: 863
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 827
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 859
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)