BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbS20010 (420 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd... 54 5e-08 At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 53 7e-08 At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd... 52 2e-07 At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd... 49 1e-06 At5g46570.1 68418.m05734 protein kinase family protein contains ... 32 0.18 At1g64330.1 68414.m07290 myosin heavy chain-related similar to m... 28 3.0 >At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 299 Score = 53.6 bits (123), Expect = 5e-08 Identities = 27/75 (36%), Positives = 46/75 (61%) Frame = +3 Query: 33 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKI 212 A+II G +A +I +++ ++V L K G P LA+V VG R+DS Y+ K KA ++ Sbjct: 9 AKIIDGKAIAHTIRSEIAEEVRGLSEK-HGKVPGLAVVIVGSRKDSQTYVNTKRKACAEV 67 Query: 213 GIAAEHIRLPRDITK 257 GI + + LP ++++ Sbjct: 68 GIKSFDVGLPEEVSE 82 Score = 52.0 bits (119), Expect = 2e-07 Identities = 24/52 (46%), Positives = 36/52 (69%) Frame = +2 Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415 +L++K+ LN +P VHGI+VQ+PL H + H + A+S DKDVDG + +N Sbjct: 84 DLISKVHELNSNPDVHGILVQLPLPK-HINEEH-ILGAISIDKDVDGFHPLN 133 >At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 360 Score = 53.2 bits (122), Expect = 7e-08 Identities = 24/52 (46%), Positives = 36/52 (69%) Frame = +2 Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415 E+L ++ N+ PSVHGI+VQ+PL S +D + +AVS +KDVDG + +N Sbjct: 144 EVLKSVSGFNDDPSVHGILVQLPLPSH--MDEQNILNAVSIEKDVDGFHPLN 193 Score = 52.4 bits (120), Expect = 1e-07 Identities = 27/72 (37%), Positives = 43/72 (59%) Frame = +3 Query: 33 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKI 212 A +I G VA I +++ +V+R++ G P LA++ VG R+DS Y+R K KA + + Sbjct: 69 AIVIDGKAVAKKIRDEITIEVSRMKES-IGVIPGLAVILVGDRKDSATYVRNKKKACDSV 127 Query: 213 GIAAEHIRLPRD 248 GI + +RL D Sbjct: 128 GIKSFEVRLAED 139 >At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 310 Score = 52.0 bits (119), Expect = 2e-07 Identities = 23/52 (44%), Positives = 36/52 (69%) Frame = +2 Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415 E+L ++ N+ PSVHG++VQ+PL S +D + +AVS +KDVDG + +N Sbjct: 94 EVLKYVSGFNDDPSVHGVLVQLPLPSH--MDEQNILNAVSIEKDVDGFHPLN 143 >At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohydrolase, putative similar to SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate synthetase (EC 6.3.4.3) {Saccharomyces cerevisiae}; contains Pfam profiles PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain, PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain Length = 352 Score = 49.2 bits (112), Expect = 1e-06 Identities = 27/73 (36%), Positives = 41/73 (56%) Frame = +3 Query: 39 IISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGI 218 +I G +A I + +V +++ K G P LA+V VG + DS Y+R K+KA E+ GI Sbjct: 65 VIDGNVIAEEIRTKIISEVGKMK-KAVGKVPGLAVVLVGEQRDSQTYVRNKIKACEETGI 123 Query: 219 AAEHIRLPRDITK 257 + LP D T+ Sbjct: 124 KSVLAELPEDCTE 136 Score = 41.1 bits (92), Expect = 3e-04 Identities = 17/52 (32%), Positives = 33/52 (63%) Frame = +2 Query: 260 ELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTIN 415 ++++ + NE S+HGI+VQ+PL ++ ++ + V +KDVDG + +N Sbjct: 138 QIISVLRKFNEDTSIHGILVQLPLPQH--LNESKILNMVRLEKDVDGFHPLN 187 >At5g46570.1 68418.m05734 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 489 Score = 31.9 bits (69), Expect = 0.18 Identities = 19/59 (32%), Positives = 29/59 (49%) Frame = +3 Query: 42 ISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGI 218 ++G +V I+N ++ LR +GF P IV GG + NV R KL+ + I Sbjct: 26 VNGDQVDQEIQNFKEFELNELRKATNGFSPS-CIVSEGGEKAPNVVYRGKLEGNHLVAI 83 >At1g64330.1 68414.m07290 myosin heavy chain-related similar to myosin heavy chain (GI:1850913) [Entamoeba histolytica]; similar to Intracellular protein transport protein USO1 (Swiss-Prot:P25386) [Saccharomyces cerevisiae] Length = 555 Score = 27.9 bits (59), Expect = 3.0 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +3 Query: 51 IEVAGSIENDLRQQVTRLRSKWSGFEPRLA 140 +EVAG E+DL Q++ ++ + G E LA Sbjct: 190 LEVAGETESDLNQKLEDVKKERDGLEAELA 219 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,137,647 Number of Sequences: 28952 Number of extensions: 142737 Number of successful extensions: 358 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 347 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 354 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 645327280 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -