Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32531
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 66 3e-12
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 64 2e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 50 3e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 30 0.18
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr... 28 0.95
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 26 3.8
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 3.8
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 25 6.7
SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 8.9
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 66.1 bits (154), Expect = 3e-12
Identities = 41/138 (29%), Positives = 69/138 (50%), Gaps = 1/138 (0%)
Frame = +2
Query: 101 DLSKNDVERASXSFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXX 280
+L+ + +FS++D + G I + LG ++R+L +PT A +
Sbjct: 5 NLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGT 64
Query: 281 XXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSE 457
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L E
Sbjct: 65 IDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 458 VAEVTKDCMDPEDDDGMI 511
VA++ ++ D DG+I
Sbjct: 123 VADMIREA--DTDGDGVI 138
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 63.7 bits (148), Expect = 2e-11
Identities = 37/113 (32%), Positives = 60/113 (53%)
Frame = +2
Query: 137 SFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 316
+FS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 317 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTK 475
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLK 120
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 49.6 bits (113), Expect = 3e-07
Identities = 31/128 (24%), Positives = 61/128 (47%)
Frame = +2
Query: 107 SKNDVERASXSFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 286
SK + +F +YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 287 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 466
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 467 VTKDCMDP 490
+ ++ DP
Sbjct: 119 MVQEA-DP 125
Score = 30.3 bits (65), Expect = 0.18
Identities = 14/52 (26%), Positives = 31/52 (59%)
Frame = +2
Query: 344 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 499
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDE 60
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 30.3 bits (65), Expect = 0.18
Identities = 31/151 (20%), Positives = 59/151 (39%), Gaps = 1/151 (0%)
Frame = +2
Query: 62 RPGGNRQTPHKMSDLSKNDVERASXSFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 241
R GG ++++ + D+ A F ++D + ID L +RAL N + +
Sbjct: 20 RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76
Query: 242 XXXXXXXXXXXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 418
F+ + ++ ++D E+ +L+D +E G + L
Sbjct: 77 KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134
Query: 419 TLLALGEKLDDSEVAEVTKDCMDPEDDDGMI 511
L E +DD E+ + ++ D DG I
Sbjct: 135 VAKELNENIDDQELEAMIEEF--DLDQDGEI 163
>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 178
Score = 27.9 bits (59), Expect = 0.95
Identities = 22/97 (22%), Positives = 44/97 (45%), Gaps = 2/97 (2%)
Frame = +3
Query: 69 EGTD-KHHTK*ATSARTTLKGRLXPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSR 242
E TD ++HT T A+ KG P++ KAK+ + ++ +S Q P++
Sbjct: 38 ERTDYRNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTK 97
Query: 243 NSVVQRRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 353
+S+ + + + SK S+ ++ + +IS
Sbjct: 98 HSLDENEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134
>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 371
Score = 25.8 bits (54), Expect = 3.8
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 119 VERASXSFSIYDFEGKGKIDAFNLG 193
+E A + SIYDF +I+A N+G
Sbjct: 117 IEDAPPTQSIYDFSSSRQINALNVG 141
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 3.8
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +2
Query: 431 LGEKLDDSEVAEVTKDCMDPED 496
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 25.0 bits (52), Expect = 6.7
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = -1
Query: 234 VASVGFELSALRRSPRLKASILPLPSKS*IEKXKDALSTSFLLRSLIL 91
V S GF SA + A + PS++ E K+AL FL IL
Sbjct: 77 VISTGFAWSAYSLVKKYIAPMFRAPSQNAYEADKNALDAKFLEAHKIL 124
>SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit
Swi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +1
Query: 46 ERLPVKTGREPTNTTQNERPQQ 111
E +PV TG E ++ NER +Q
Sbjct: 8 ESIPVNTGSESISSNDNERFEQ 29
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 24.6 bits (51), Expect = 8.9
Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 2/75 (2%)
Frame = +3
Query: 114 TTLKGRLXPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 287
T PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 288 KSSFPSTAKQRKTKT 332
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,009,499
Number of Sequences: 5004
Number of extensions: 37117
Number of successful extensions: 116
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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