Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32508
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 80 2e-16
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 77 1e-15
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 54 1e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 40 2e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 38 0.001
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 2.9
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 5.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 79.8 bits (188), Expect = 2e-16
Identities = 44/110 (40%), Positives = 65/110 (59%), Gaps = 6/110 (5%)
Frame = +1
Query: 205 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXELDEMVGE----ASGPIN 372
+ +Q+AEF+EAF L D D+DG I N+L SLG+ + EL +M+ E +G I+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 373 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLXHALMTWG 516
FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G
Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLG 115
Score = 36.3 bits (80), Expect = 0.003
Identities = 20/65 (30%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +1
Query: 223 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXELDEMVGEA----SGPINFTQLLT 390
E +EAF++ D D +G I +L SLG S+ E+ +M+ EA G IN+ +
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSR 145
Query: 391 LFANR 405
+ +++
Sbjct: 146 VISSK 150
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 77.4 bits (182), Expect = 1e-15
Identities = 43/121 (35%), Positives = 64/121 (52%), Gaps = 2/121 (1%)
Frame = +1
Query: 160 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXELD 339
++ AKR S F+ + Q+ E KEAF L+D D DG IG+ D++ SL + ASE ++
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87
Query: 340 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLXHALMTW 513
M + PIN LT + + S +D ++ AF TFD + GKI + AL +
Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146
Query: 514 G 516
G
Sbjct: 147 G 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 54.4 bits (125), Expect = 1e-08
Identities = 32/106 (30%), Positives = 58/106 (54%), Gaps = 2/106 (1%)
Frame = +1
Query: 205 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXELDEMVGEASGPINFTQL 384
S++Q E KEAF L D DKDG+I + + + SLG ++ EL ++ E I+ +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 385 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLXHALMTWG 516
++ +N++ E++ I AF+ FD++ G I++ + + T G
Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLG 108
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 39.9 bits (89), Expect = 2e-04
Identities = 18/42 (42%), Positives = 25/42 (59%)
Frame = +1
Query: 223 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXELDEMV 348
EF + FQ+ D D G+IG +LR SLG S E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 37.5 bits (83), Expect = 0.001
Identities = 28/102 (27%), Positives = 50/102 (49%), Gaps = 6/102 (5%)
Frame = +1
Query: 205 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXELDEMVGE----ASGPIN 372
+++Q + EAF+L D DKD I ++LRA +LG A + E+ +++ + G +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 373 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERL 492
+ + ++ D + + AF+ F DE GKI L
Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNL 132
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 26.2 bits (55), Expect = 2.9
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +1
Query: 223 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXELDEMV 348
E +EAF L D G I DLR + LG ++ +L M+
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 25.4 bits (53), Expect = 5.1
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +1
Query: 214 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEXE 333
+ A+FK+ F DKD + + +L A + L +LA + +
Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEKFD 251
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.132 0.372
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,485,299
Number of Sequences: 5004
Number of extensions: 21359
Number of successful extensions: 98
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 97
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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