Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32506
(401 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 197 6e-52
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 111 4e-26
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 108 3e-25
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 105 2e-24
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.1
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 1.9
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 3.3
SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosacc... 25 5.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 197 bits (480), Expect = 6e-52
Identities = 83/110 (75%), Positives = 96/110 (87%)
Frame = +1
Query: 70 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 249
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 250 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTXGA 399
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYT GA
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGA 110
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 111 bits (267), Expect = 4e-26
Identities = 50/111 (45%), Positives = 69/111 (62%), Gaps = 2/111 (1%)
Frame = +1
Query: 70 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 243
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 244 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTXG 396
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVG 111
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 108 bits (260), Expect = 3e-25
Identities = 45/106 (42%), Positives = 74/106 (69%), Gaps = 2/106 (1%)
Frame = +1
Query: 73 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 252
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 253 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGH 384
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGY 108
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 105 bits (253), Expect = 2e-24
Identities = 48/115 (41%), Positives = 68/115 (59%), Gaps = 6/115 (5%)
Frame = +1
Query: 70 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 231
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 232 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTXG 396
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVG 115
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.1
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 109 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 210
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.2 bits (55), Expect = 1.9
Identities = 10/25 (40%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +1
Query: 304 GPFGQIFRPDNFVFGQSGAG-NNWA 375
GP+G +F P F+F +G NW+
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWS 181
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 3.3
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 362 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 267
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPCC10H11.01 |prp11||ATP-dependent RNA helicase
Prp11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1014
Score = 24.6 bits (51), Expect = 5.8
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -3
Query: 354 GLSEDEVVRTEDLSERSRADRVHGAGLQVDEDG 256
G+S E+ R +D S+ R +RVH DEDG
Sbjct: 240 GISGFEINRQKDTSDMKRNNRVH----MDDEDG 268
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,458,582
Number of Sequences: 5004
Number of extensions: 26408
Number of successful extensions: 84
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 81
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 136158338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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