Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32482
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 320 6e-89
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 182 2e-47
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 168 5e-43
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 98 8e-22
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 97 2e-21
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 86 3e-18
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 49 2e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 1e-08
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 2.2
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 26 3.8
SPBC16A3.08c |||nuclear telomere cap complex subunit |Schizosacc... 25 5.1
SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5 |Schizosacchar... 25 6.7
SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 8.9
SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces pombe... 25 8.9
SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 8.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 320 bits (787), Expect = 6e-89
Identities = 150/172 (87%), Positives = 161/172 (93%)
Frame = +1
Query: 1 DGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKL 180
DGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKL
Sbjct: 157 DGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKL 216
Query: 181 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHE 360
CYVALDFEQE+ TAA S+SLEKSYELPDGQVITIGNERFR PEALFQPS LG+E+ GIHE
Sbjct: 217 CYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENAGIHE 276
Query: 361 TVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIK 516
YNSIMKCDVDIRKDLY N VMSGGTTMYPGIADRMQKEI ALAPS++K+K
Sbjct: 277 ATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVK 328
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 182 bits (444), Expect = 2e-47
Identities = 82/164 (50%), Positives = 115/164 (70%)
Frame = +1
Query: 1 DGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKL 180
DGV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F TA+ E VR++KEKL
Sbjct: 157 DGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKL 216
Query: 181 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHE 360
CYV+ D E + + +T L ++Y LPDG+VI +G+ER+ CPE LFQP +G E G+ E
Sbjct: 217 CYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSE 276
Query: 361 TVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITAL 492
++++I DVDIRK LY V+SGG++MY G+ R++KEI L
Sbjct: 277 FIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQL 320
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 168 bits (408), Expect = 5e-43
Identities = 74/172 (43%), Positives = 114/172 (66%)
Frame = +1
Query: 1 DGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKL 180
DG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++AE+EIVR+IKEK
Sbjct: 162 DGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKC 221
Query: 181 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHE 360
CYVA DF E+ + +Y+LPD Q IT+G E F PE LF P +G E+ G+H
Sbjct: 222 CYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHI 281
Query: 361 TVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTIKIK 516
++ SI+ D+D+R LY+N V+SGG+T+ G +R E+ A++ ++K
Sbjct: 282 QLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVK 333
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 97.9 bits (233), Expect = 8e-22
Identities = 60/189 (31%), Positives = 96/189 (50%), Gaps = 20/189 (10%)
Frame = +1
Query: 10 SHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYV 189
+H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV
Sbjct: 168 THIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNMME--ETYIVNEIKESVCYV 225
Query: 190 ALDFEQEMATAAAS--TSLEKSYELPD------------------GQVITIGNERFRCPE 309
+ +F+++M + LE Y LPD QV+ + NERF PE
Sbjct: 226 SQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIEQQVLNLSNERFMIPE 285
Query: 310 ALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITA 489
LF PS + + GI E V S+ +I+ L N V GG +PG R+ E+ +
Sbjct: 286 LLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRS 345
Query: 490 LAPSTIKIK 516
LAP+ ++K
Sbjct: 346 LAPANWEVK 354
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 96.7 bits (230), Expect = 2e-21
Identities = 59/176 (33%), Positives = 91/176 (51%), Gaps = 7/176 (3%)
Frame = +1
Query: 1 DGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKE 174
DGV+H +P+ EGY + +I + LAGRD+T ++ +L +R S TAER IKE
Sbjct: 188 DGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKE 242
Query: 175 KLCYVALDFEQEMA--TAAASTSLEKSYELPDGQ--VITIGNERFRCPEALFQPSFLGME 342
+ CYV D +E + L+ + E G I +G ERF PE F P +
Sbjct: 243 ECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIASSD 302
Query: 343 -SCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAPSTI 507
+ E V N + +D+RK LY N V+SGG+T++ +R+Q+++ + I
Sbjct: 303 FLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERI 358
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 85.8 bits (203), Expect = 3e-18
Identities = 48/132 (36%), Positives = 70/132 (53%), Gaps = 9/132 (6%)
Frame = +1
Query: 127 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNERFRCP 306
S+ +R ++ + KE + V + A AS+ K +E PDG G ERFR
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV---LDTPFDEAKASSRNPKPFEFPDGVTHKFGQERFRIS 299
Query: 307 EALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGI 459
E LF PSF S G+HE VY SI+ CD ++R L N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359
Query: 460 ADRMQKEITALA 495
++R+Q E+ LA
Sbjct: 360 SERLQAEVQRLA 371
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 48.8 bits (111), Expect(2) = 2e-11
Identities = 24/56 (42%), Positives = 35/56 (62%), Gaps = 1/56 (1%)
Frame = +1
Query: 349 GIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADRMQKEITALAP-STIKI 513
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P S +KI
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGSRLKI 381
Score = 34.3 bits (75), Expect(2) = 2e-11
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +1
Query: 220 AAASTSLEKSYELPDGQVITIGNERFRCPEALFQP 324
++ +T E +E PDG + G ER++ PE LF P
Sbjct: 254 SSETTIAESEFEFPDGSRMMFGAERYQIPEHLFVP 288
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 54.0 bits (124), Expect = 1e-08
Identities = 21/70 (30%), Positives = 45/70 (64%)
Frame = +1
Query: 289 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYANTVMSGGTTMYPGIADR 468
E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 469 MQKEITALAP 498
+++E+T++ P
Sbjct: 654 IKRELTSIMP 663
Score = 31.9 bits (69), Expect = 0.059
Identities = 18/82 (21%), Positives = 34/82 (41%)
Frame = +1
Query: 1 DGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKL 180
+ SH +P+ G + R+ G + YL+K+ + SF +
Sbjct: 174 NAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDH 233
Query: 181 CYVALDFEQEMATAAASTSLEK 246
C+V+ D+ ++A A LE+
Sbjct: 234 CHVSSDYTHDIAHALDRDILER 255
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 2.2
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2
Query: 26 STKVTLCPTPSSVWTW 73
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.8 bits (54), Expect = 3.8
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = +1
Query: 403 KDLYANTVMSGGTTMYPGIADRMQKEI 483
K+LY++ ++ GG +PG A +++ I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERI 553
Score = 25.4 bits (53), Expect = 5.1
Identities = 9/36 (25%), Positives = 21/36 (58%)
Frame = +1
Query: 25 IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 132
+ EG +P++ ++++ G D+T MK+L + +
Sbjct: 308 VEEGVVVPNSRIKINYGGDDITLLFMKLLMRSHFPY 343
>SPBC16A3.08c |||nuclear telomere cap complex subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 284
Score = 25.4 bits (53), Expect = 5.1
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +3
Query: 354 PRDRVQLHHEVRRRHP*GPVRQHRHVRWYHHVPRYRRQDAEGD 482
PR+ V R+R P P + R V PR RRQ +G+
Sbjct: 41 PRELVAQSTTSRKRDPNQPTPRERTVNKKADQPRRRRQAPQGN 83
>SPAC18B11.06 |||U3 snoRNP-associated protein Lcp5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 327
Score = 25.0 bits (52), Expect = 6.7
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +3
Query: 27 LRRLRSAPRHPPSGLGWSRLDRLPHED 107
L++ + +H G WS LDR H+D
Sbjct: 254 LKKSKRVKKHDYGGEDWSLLDRKFHDD 280
>SPBC18E5.09c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 128
Score = 24.6 bits (51), Expect = 8.9
Identities = 13/43 (30%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = +1
Query: 181 CYVALDFEQEMATAAASTSLEKSYELPDGQVITIGNE-RFRCP 306
CYV+ +E + + SL SY +PD + I + R+ P
Sbjct: 39 CYVSRPLTKERSCSHPYISLGSSYGIPDAENIEYPRDARYHSP 81
>SPCC970.03 |||cytochrome b5 reductase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 301
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/16 (62%), Positives = 11/16 (68%), Gaps = 1/16 (6%)
Frame = +2
Query: 11 PTPYPSTKVTLC-PTP 55
P P P TKV +C PTP
Sbjct: 256 PAPSPETKVLICGPTP 271
>SPAC23H4.13c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 24.6 bits (51), Expect = 8.9
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +1
Query: 166 IKEKLCYVALDFEQEMATAAASTSLEKSYELPDGQV 273
IKEKL VA EQE A +SL+ S + G V
Sbjct: 111 IKEKLRRVANITEQEQQCIPAESSLDLSIQAMKGVV 146
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,865,214
Number of Sequences: 5004
Number of extensions: 35330
Number of successful extensions: 143
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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