Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32443
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 324 6e-90
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 185 4e-48
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 174 7e-45
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 132 2e-32
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 99 2e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 99 2e-22
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 89 5e-19
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 49 4e-07
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 36 0.005
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.10
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.18
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.9
SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter family|Sch... 25 6.7
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 6.7
SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.9
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 8.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 324 bits (795), Expect = 6e-90
Identities = 145/160 (90%), Positives = 156/160 (97%)
Frame = +1
Query: 37 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 216
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 217 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 396
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 397 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 516
I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+H
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTH 161
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 185 bits (450), Expect = 4e-48
Identities = 85/159 (53%), Positives = 114/159 (71%), Gaps = 1/159 (0%)
Frame = +1
Query: 43 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 222
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 223 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTQI 399
+L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+ +
Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127
Query: 400 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 516
+ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 174 bits (423), Expect = 7e-45
Identities = 81/158 (51%), Positives = 115/158 (72%), Gaps = 2/158 (1%)
Frame = +1
Query: 49 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 222
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 223 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 402
+L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M
Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123
Query: 403 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 516
FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTH 161
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 132 bits (320), Expect = 2e-32
Identities = 77/186 (41%), Positives = 104/186 (55%), Gaps = 32/186 (17%)
Frame = +1
Query: 55 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 183
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 184 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 342
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 343 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 498
LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186
Query: 499 GDGVSH 516
GDGV+H
Sbjct: 187 GDGVTH 192
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 99 bits (238), Expect = 2e-22
Identities = 55/167 (32%), Positives = 98/167 (58%), Gaps = 3/167 (1%)
Frame = +1
Query: 25 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 204
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGND 72
Query: 205 AQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 381
+ G ++ + P E +I+NWD ME++ +TF +L + EHP+ +TE NP R
Sbjct: 73 TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131
Query: 382 EKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSH 516
MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH
Sbjct: 132 STMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASH 178
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 99 bits (238), Expect = 2e-22
Identities = 54/154 (35%), Positives = 84/154 (54%)
Frame = +1
Query: 40 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 219
D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 220 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 399
G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K +
Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 400 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 501
MFE+ PA Y+A Q + +ASG+ T ++D G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIG 154
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 88.6 bits (210), Expect = 5e-19
Identities = 46/156 (29%), Positives = 87/156 (55%), Gaps = 2/156 (1%)
Frame = +1
Query: 40 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 219
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 220 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 393
I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121
Query: 394 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 501
+ FE + PA Y+ QAV +A+ ++T +++D G
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIG 157
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 49.2 bits (112), Expect = 4e-07
Identities = 28/91 (30%), Positives = 44/91 (48%), Gaps = 4/91 (4%)
Frame = +1
Query: 241 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 408
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 409 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSG 501
P +A + ++ LYA G G+V+D G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 35.5 bits (78), Expect = 0.005
Identities = 35/146 (23%), Positives = 60/146 (41%), Gaps = 4/146 (2%)
Frame = +1
Query: 55 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 234
+V+DNG+ KAGFAG P+ + R + G + ++G+E + TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 235 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 402
++ H G + +W +W N + P ++ +LLT+ + Q++
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117
Query: 403 FETFNSPAMYVAIQAVLSLYASGRTT 480
FE F A A L + G T
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFT 143
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.10
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +1
Query: 100 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 243
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.18
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +1
Query: 301 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 444
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 2.9
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 343 LLTEAPLNPKANREKMTQIMFETFN 417
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC1840.12 ||SPCC965.02|OPT oligopeptide transporter
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 791
Score = 25.0 bits (52), Expect = 6.7
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = -2
Query: 296 WCQIFS-MSSQLVMIPCSMGYFRVRIPLLL-WASSPT*ESFWPIPTITPWW 150
WC+ S + SQ+ + +G F PL L WA + + P ITPWW
Sbjct: 304 WCKPSSAVLSQIFGVKTGLGLF----PLTLDWAQISSLSN----PLITPWW 346
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 25.0 bits (52), Expect = 6.7
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 160 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 246
+M+G +Y+G AQ KR +LT + I
Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193
>SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 485
Score = 24.6 bits (51), Expect = 8.9
Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 5/56 (8%)
Frame = +1
Query: 67 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMG-----QKDSYVGDEAQSKR 219
N +GM + R F S VG + VG Q+DSY+ D++ S+R
Sbjct: 318 NATGMYGVSKGHSSSTRRPFFSDVGSSQPAEEFVGSSSSHGRQQDSYIADDSDSER 373
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 24.6 bits (51), Expect = 8.9
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = -1
Query: 264 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHH 160
GDD+VLD V +D L L AH D H
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,924,709
Number of Sequences: 5004
Number of extensions: 39175
Number of successful extensions: 141
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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