Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32427
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 223 2e-59
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 168 5e-43
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 156 2e-39
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 99 2e-22
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 40 2e-04
SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|ch... 30 0.18
SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 29 0.31
SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 27 1.3
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 2.2
SPAPB1A11.04c |||transcription factor |Schizosaccharomyces pombe... 26 3.8
SPAC343.06c |||scramblase|Schizosaccharomyces pombe|chr 1|||Manual 25 8.9
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 223 bits (544), Expect = 2e-59
Identities = 103/173 (59%), Positives = 134/173 (77%), Gaps = 3/173 (1%)
Frame = +2
Query: 5 RKKVTAVHKANIMRMSDGLFLRCCRELAXKYPDIKFEERYLDTVCLNMVQDPSKFD--VL 178
+ +T VHKA IMRM+DGLFL C +ELA +YPDI+ E LD CL +V DP ++ V+
Sbjct: 205 KNNITVVHKATIMRMADGLFLECAKELAPEYPDIELREEILDNACLKIVTDPVPYNNTVM 264
Query: 179 VMPNLYGDIMSDMCSGLVGGLGLTPSGNIGKNGALFESVHGTAPDIAGKDMANPTALLLS 358
VMPNLYGDI+SDMC+GL+GGLGLTPSGNIG ++FE+VHGTAPDIAGK +ANPTALLLS
Sbjct: 265 VMPNLYGDIVSDMCAGLIGGLGLTPSGNIGNQASIFEAVHGTAPDIAGKGLANPTALLLS 324
Query: 359 AIMMLRHLQLNDHADLVQNACYEVLXEG-RALTGDLGGTAKCSEFTNAIISKL 514
++MML+H+ LND+A +++A ++ L A T DLGG + ++T+AIISKL
Sbjct: 325 SVMMLKHMNLNDYAKRIESAIFDTLANNPDARTKDLGGKSNNVQYTDAIISKL 377
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 168 bits (408), Expect = 5e-43
Identities = 83/171 (48%), Positives = 113/171 (66%), Gaps = 1/171 (0%)
Frame = +2
Query: 5 RKKVTAVHKANIMRMSDGLFLRCCRELAXKYPDIKFEERYLDTVCLNMVQDPSKFDVLVM 184
RK VT +HKANIM+++DGLF R ++A Y I ++ +D + V P +FDVLVM
Sbjct: 183 RKSVTCIHKANIMKLADGLFRRTFYDVANGYDAITPKDLIVDNASMQAVSRPQQFDVLVM 242
Query: 185 PNLYGDIMSDMCSGLVGGLGLTPSGNIGKNGALFE-SVHGTAPDIAGKDMANPTALLLSA 361
PNLYG I+S++ S LVGG G+ P N G++ ALFE I G+ ANPTA +LSA
Sbjct: 243 PNLYGSILSNIGSALVGGPGVIPGANFGRDYALFEPGCRHVGLSITGRGEANPTAAILSA 302
Query: 362 IMMLRHLQLNDHADLVQNACYEVLXEGRALTGDLGGTAKCSEFTNAIISKL 514
+MLRHL L D+ADL+ A Y V+ EG+ LT DLGG+A +FT+AI+ ++
Sbjct: 303 CLMLRHLGLKDYADLINAATYSVIEEGKTLTKDLGGSASTGDFTHAILERM 353
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 156 bits (378), Expect = 2e-39
Identities = 75/174 (43%), Positives = 110/174 (63%), Gaps = 3/174 (1%)
Frame = +2
Query: 2 KRKKVTAVHKANIMRMSDGLFLRCCRE---LAXKYPDIKFEERYLDTVCLNMVQDPSKFD 172
K+ VT +HK+N+M ++DGLF CR L Y I +E+ +D++ + ++P FD
Sbjct: 187 KKPLVTIIHKSNVMSVTDGLFRESCRHAQSLDPSYASINVDEQIVDSMVYRLFREPECFD 246
Query: 173 VLVMPNLYGDIMSDMCSGLVGGLGLTPSGNIGKNGALFESVHGTAPDIAGKDMANPTALL 352
V+V PNLYGDI+SD + L+G LGL PS N+G N + E VHG+APDIAG+ +ANP A
Sbjct: 247 VVVAPNLYGDILSDGAASLIGSLGLVPSANVGDNFVMSEPVHGSAPDIAGRGIANPVATF 306
Query: 353 LSAIMMLRHLQLNDHADLVQNACYEVLXEGRALTGDLGGTAKCSEFTNAIISKL 514
S +ML + D A + A +VL EG+ LT DLGG + +E T+A+++ +
Sbjct: 307 RSVALMLEFMGHQDAAADIYTAVDKVLTEGKVLTPDLGGKSGTNEITDAVLANI 360
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 99 bits (238), Expect = 2e-22
Identities = 64/182 (35%), Positives = 102/182 (56%), Gaps = 15/182 (8%)
Frame = +2
Query: 14 VTAVHKANIMRMSDGLFLRCCRELAXKYPDIKFEERYLDTVCLNMVQDPSKFD-VLVMPN 190
VT + KAN++ S + +YP + + + +D+ + +V+ P + V++ N
Sbjct: 186 VTLLDKANVLATSRLWRKTVAKIFKEEYPHLTLKNQLIDSAAMLLVKSPRTLNGVVLTDN 245
Query: 191 LYGDIMSDMCSGLVGGLGLTPS----GNIGKNG----ALFESVHGTAPDIAGKDMANPTA 346
L+GDI+SD S + G LGL PS G +GK+ L E +HG+APDIAGK + NP
Sbjct: 246 LFGDIISDEASVIPGSLGLLPSASLSGVVGKSEEKVHCLVEPIHGSAPDIAGKGIVNPVG 305
Query: 347 LLLSAIMMLRH-LQLNDHADLVQNACYEVLXE----GRAL-TGDLGGTAKCSEFTNAIIS 508
+LSA ++LR+ L A+ ++ A +VL + GR L T DLGG A ++ T A++
Sbjct: 306 TILSASLLLRYGLNAPKEAEAIEAAVRKVLDDTSIGGRGLYTRDLGGEASTADITKAVVE 365
Query: 509 KL 514
+L
Sbjct: 366 EL 367
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 40.3 bits (90), Expect = 2e-04
Identities = 34/101 (33%), Positives = 45/101 (44%), Gaps = 9/101 (8%)
Frame = +2
Query: 29 KANIMRMSDGLFLRCCRELAXKYPDIKFEE-------RYLDTVCLNMVQDPSKFDVLVMP 187
K I++ DG F +E+ KFEE R +D + ++ F V
Sbjct: 222 KNTILKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMVAQAIKSNGGF-VWACK 280
Query: 188 NLYGDIMSDMCSGLVGGLGLTPSGNIGKNGALFES--VHGT 304
N GD+MSD+ + G LGL S I NG FES HGT
Sbjct: 281 NYDGDVMSDVVAQAYGSLGLMTSVLIHPNGRTFESEAAHGT 321
>SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 463
Score = 30.3 bits (65), Expect = 0.18
Identities = 17/60 (28%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -1
Query: 372 SIIIADNSRAVGLAMSLPAISGAVPCTDSKSAPFLPMLPEGVRPSPPTSPE-HMSDMMSP 196
S ++A ++ + + + A S + T S SA P+ P G+R T PE + ++ SP
Sbjct: 276 SSVVAYTTKCIVVPVITTAASQSEAATPSPSAAVYPLFPHGIRLIDSTKPEANSGNVYSP 335
>SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 413
Score = 29.5 bits (63), Expect = 0.31
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -1
Query: 213 SDMMSPYRLGITSTSNLEGSCTMLRQTVSRYRSSNLMSGYLXASSRQ 73
+ + P+RL L+ +CTM R T R+ S G+ ASSR+
Sbjct: 204 NSLSEPFRLSKPKLDFLKHACTMFRGT-HRFHSYTTEKGFSDASSRR 249
>SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 1.3
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -1
Query: 492 VNSLHXAVPPRSPVKALPSXNTS*QAFW 409
+N + PP P+K + S NTS Q FW
Sbjct: 193 INPPYVYGPPIHPMKNMDSLNTSNQIFW 220
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.6 bits (56), Expect = 2.2
Identities = 15/35 (42%), Positives = 18/35 (51%)
Frame = -1
Query: 327 SLPAISGAVPCTDSKSAPFLPMLPEGVRPSPPTSP 223
SLP + A P SAP P LP G+ +PP P
Sbjct: 438 SLPMGAPAAPPLPP-SAPIAPPLPAGMPAAPPLPP 471
>SPAPB1A11.04c |||transcription factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 697
Score = 25.8 bits (54), Expect = 3.8
Identities = 22/86 (25%), Positives = 35/86 (40%)
Frame = -1
Query: 351 SRAVGLAMSLPAISGAVPCTDSKSAPFLPMLPEGVRPSPPTSPEHMSDMMSPYRLGITST 172
S+ L++SL S +PC + P+L E S P + + + M+PY+ +
Sbjct: 290 SKLRSLSLSLQKTSIQLPCPEPNFLFNQPILTELFDGSLPENTQSDTTSMAPYQRSL-QL 348
Query: 171 SNLEGSCTMLRQTVSRYRSSNLMSGY 94
+ GS L S L GY
Sbjct: 349 EFMTGSLIRLSNLWSEISRWALCGGY 374
>SPAC343.06c |||scramblase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 381
Score = 24.6 bits (51), Expect = 8.9
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = +2
Query: 254 SGNIGKNGALFESVHGTAPDIAGKDMA 334
+G++ +N L ++ HG A D+ +DM+
Sbjct: 258 NGSVNEN-QLLQAAHGIANDVCARDMS 283
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,823,552
Number of Sequences: 5004
Number of extensions: 32837
Number of successful extensions: 128
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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