Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32349
(497 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 68 9e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 66 3e-12
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 52 5e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 30 0.22
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 3.6
SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyce... 25 4.8
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 4.8
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.3
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 6.3
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 67.7 bits (158), Expect = 9e-13
Identities = 46/142 (32%), Positives = 70/142 (49%), Gaps = 3/142 (2%)
Frame = +2
Query: 29 TSAXNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXX 202
T D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 3 TRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGN 62
Query: 203 XXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDD 379
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 63 GTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQ 120
Query: 380 SEVAEVTKDCMDPEDDDGXIPY 445
EVA++ ++ D DG I Y
Sbjct: 121 EEVADMIREA--DTDGDGVINY 140
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 66.1 bits (154), Expect = 3e-12
Identities = 42/136 (30%), Positives = 69/136 (50%)
Frame = +2
Query: 65 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 244
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 245 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 424
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 425 DDGXIPYAXXLKKVMA 472
DG + Y ++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 52.0 bits (119), Expect = 5e-08
Identities = 34/136 (25%), Positives = 67/136 (49%)
Frame = +2
Query: 65 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 244
AF +YD + G I ++G +LR+L N T A + F+ +
Sbjct: 14 AFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFV-----S 68
Query: 245 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 424
K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP
Sbjct: 69 NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-T 126
Query: 425 DDGXIPYAXXLKKVMA 472
+ G Y ++++MA
Sbjct: 127 NSGSFDYYDFVQRIMA 142
Score = 30.3 bits (65), Expect = 0.17
Identities = 14/52 (26%), Positives = 31/52 (59%)
Frame = +2
Query: 272 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 427
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDE 60
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.9 bits (64), Expect = 0.22
Identities = 27/126 (21%), Positives = 49/126 (38%), Gaps = 1/126 (0%)
Frame = +2
Query: 65 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 241
AF ++D + ID L +RAL N + + F+ + ++
Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101
Query: 242 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 421
++D E+ +L+D +E G + L L E +DD E+ + ++
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157
Query: 422 DDDGXI 439
D DG I
Sbjct: 158 DQDGEI 163
Score = 29.5 bits (63), Expect = 0.29
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +2
Query: 233 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 406
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 25.4 bits (53), Expect = 4.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 47 VERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 148
+E AF ++D + GKI NL + + LN N
Sbjct: 109 LEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 3.6
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +2
Query: 359 LGEKLDDSEVAEVTKDCMDPED 424
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 413
Score = 25.4 bits (53), Expect = 4.8
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +3
Query: 3 PTNTTQNERPQQXTTLKGRLSPSQSTTLKAKVRSMPST 116
PTN+T ++ + + + R S QSTTL ST
Sbjct: 126 PTNSTTSQASAKHSAVPHRSSQFQSTTLTPSTTDSSST 163
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 4.8
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +3
Query: 42 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 215
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 216 KSSFPSTAKQRKTKT 260
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 25.0 bits (52), Expect = 6.3
Identities = 19/78 (24%), Positives = 38/78 (48%)
Frame = +3
Query: 33 QQXTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSH 212
++ ++L +L+P T KAK S+ S S S+ + W + V++ A S
Sbjct: 345 REPSSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SP 401
Query: 213 SKSSFPSTAKQRKTKTRE 266
S++S P+ +K + ++
Sbjct: 402 SEASLPNLSKYSRKNAKK 419
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.0 bits (52), Expect = 6.3
Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
Frame = +2
Query: 44 DVER-ASFAFSIYDFEGKGKID 106
DV R SF F +YDF G G +D
Sbjct: 612 DVMRNISFIFELYDFNGDGFMD 633
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,760,116
Number of Sequences: 5004
Number of extensions: 30968
Number of successful extensions: 107
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 96
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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