Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32289
(358 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 144 3e-36
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 144 3e-36
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 144 3e-36
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 81 4e-17
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 76 1e-15
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 54 7e-09
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 9e-06
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 38 3e-04
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.002
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.002
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 35 0.003
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 30 0.093
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 30 0.12
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 1.1
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 26 1.5
SPAC17G8.04c |arc15|arc5, arc5|ARP2/3 actin-organizing complex s... 25 2.6
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 3.5
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 25 3.5
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 3.5
SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 4.6
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 24 6.1
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 24 6.1
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 24 6.1
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 144 bits (350), Expect = 3e-36
Identities = 67/73 (91%), Positives = 69/73 (94%)
Frame = +1
Query: 139 KRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 318
KRTIEKFEKEA E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGH
Sbjct: 36 KRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGH 95
Query: 319 RDFIKNMITXTSQ 357
RDFIKNMIT TSQ
Sbjct: 96 RDFIKNMITGTSQ 108
Score = 80.2 bits (189), Expect = 9e-17
Identities = 38/53 (71%), Positives = 42/53 (79%), Gaps = 4/53 (7%)
Frame = +3
Query: 33 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK----TYHREVREGGPGN 179
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDK + +E E G G+
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 144 bits (350), Expect = 3e-36
Identities = 67/73 (91%), Positives = 69/73 (94%)
Frame = +1
Query: 139 KRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 318
KRTIEKFEKEA E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGH
Sbjct: 36 KRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGH 95
Query: 319 RDFIKNMITXTSQ 357
RDFIKNMIT TSQ
Sbjct: 96 RDFIKNMITGTSQ 108
Score = 80.2 bits (189), Expect = 9e-17
Identities = 38/53 (71%), Positives = 42/53 (79%), Gaps = 4/53 (7%)
Frame = +3
Query: 33 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK----TYHREVREGGPGN 179
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDK + +E E G G+
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 144 bits (350), Expect = 3e-36
Identities = 67/73 (91%), Positives = 69/73 (94%)
Frame = +1
Query: 139 KRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 318
KRTIEKFEKEA E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGH
Sbjct: 36 KRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGH 95
Query: 319 RDFIKNMITXTSQ 357
RDFIKNMIT TSQ
Sbjct: 96 RDFIKNMITGTSQ 108
Score = 80.2 bits (189), Expect = 9e-17
Identities = 38/53 (71%), Positives = 42/53 (79%), Gaps = 4/53 (7%)
Frame = +3
Query: 33 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK----TYHREVREGGPGN 179
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDK + +E E G G+
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGS 53
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 81.4 bits (192), Expect = 4e-17
Identities = 37/71 (52%), Positives = 46/71 (64%)
Frame = +1
Query: 142 RTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHR 321
R+++K EA GKGSF YAW+LD + ER RG+T+D+A FE+ K I DAPGHR
Sbjct: 207 RSMQKLHNEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHR 266
Query: 322 DFIKNMITXTS 354
DFI MI S
Sbjct: 267 DFISGMIAGAS 277
Score = 39.1 bits (87), Expect = 2e-04
Identities = 19/49 (38%), Positives = 29/49 (59%), Gaps = 4/49 (8%)
Frame = +3
Query: 45 KTHINIVVIGHVDSGKSTTTGHLIYKCGGID----KTYHREVREGGPGN 179
K +++VV GHVDSGKST G ++++ G I+ + H E G G+
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGS 223
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 76.2 bits (179), Expect = 1e-15
Identities = 33/73 (45%), Positives = 48/73 (65%)
Frame = +1
Query: 139 KRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 318
KRT+EK E+EA+E GK S+ +W LD ERE+G T+++ FET +++DAPGH
Sbjct: 267 KRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGH 326
Query: 319 RDFIKNMITXTSQ 357
+ ++ NMI SQ
Sbjct: 327 KGYVTNMINGASQ 339
Score = 46.8 bits (106), Expect = 1e-06
Identities = 22/46 (47%), Positives = 30/46 (65%), Gaps = 4/46 (8%)
Frame = +3
Query: 45 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK----TYHREVREGG 170
K H+NIV IGHVD+GKST G++++ G +DK RE +E G
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAG 281
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 54.0 bits (124), Expect = 7e-09
Identities = 25/57 (43%), Positives = 36/57 (63%)
Frame = +1
Query: 175 EMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMIT 345
++G+ SF +DK E+ RGITI A ++ET+ + +D PGH D+IKNMIT
Sbjct: 79 DLGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMIT 135
Score = 35.1 bits (77), Expect = 0.003
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +3
Query: 39 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 125
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 43.6 bits (98), Expect = 9e-06
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +1
Query: 208 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 327
V+D L AER+RGITI+ A F + +ID PGH DF
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADF 106
Score = 28.7 bits (61), Expect = 0.28
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 57 NIVVIGHVDSGKSTTTGHLIYKCG 128
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 38.3 bits (85), Expect = 3e-04
Identities = 18/43 (41%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
Frame = +1
Query: 211 LDKLKAERERGITIDI----ALWKFETSKYYVTIIDAPGHRDF 327
LDKL+ ER RGIT+ ++ + Y + +ID PGH DF
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDF 137
Score = 24.6 bits (51), Expect = 4.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 57 NIVVIGHVDSGKSTTTGHLIYKCGGIDK 140
N VI H+D GKST + ++ G I++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.002
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 33 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 128
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 23.8 bits (49), Expect = 8.0
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 286 YYVTIIDAPGHRDF 327
+ V +ID+PGH DF
Sbjct: 98 FLVNLIDSPGHVDF 111
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.002
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 33 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 128
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 23.8 bits (49), Expect = 8.0
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 286 YYVTIIDAPGHRDF 327
+ V +ID+PGH DF
Sbjct: 98 FLVNLIDSPGHVDF 111
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 35.1 bits (77), Expect = 0.003
Identities = 23/58 (39%), Positives = 31/58 (53%), Gaps = 5/58 (8%)
Frame = -1
Query: 241 HAHAQPLVCPIPKHI*RILYPFPGPPSRTSRWYVLSIP----PHL*I-K*PVVVDLPE 83
H+H PL P+P +L P P P S WY +S+P PH I K PV+ +LP+
Sbjct: 135 HSHHPPLHNPLPVSCQPVLRP-PPVPQVPSHWYPVSLPSPNLPHQPISKPPVIPNLPK 191
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 30.3 bits (65), Expect = 0.093
Identities = 17/35 (48%), Positives = 22/35 (62%)
Frame = +3
Query: 57 NIVVIGHVDSGKSTTTGHLIYKCGGIDKTYHREVR 161
NI + H+DSGK+T T ++Y G I K H EVR
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI-KDIH-EVR 93
Score = 26.2 bits (55), Expect = 1.5
Identities = 22/58 (37%), Positives = 28/58 (48%), Gaps = 19/58 (32%)
Frame = +1
Query: 211 LDKLKAERERGITIDIA----LWK---------------FETSKYYVTIIDAPGHRDF 327
+D ++ ERE+GITI A W+ FE S Y + IID PGH DF
Sbjct: 102 MDFMELEREKGITIQSAATHCTWERTVDQIEANEKQKTDFEKS-YNINIIDTPGHIDF 158
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 29.9 bits (64), Expect = 0.12
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
Frame = +1
Query: 214 DKLKAER-ERGITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 336
+K++A+ E+ + I+I + E +++ T+ID PG DFI N
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 26.6 bits (56), Expect = 1.1
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = -3
Query: 275 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFSMV 144
S F IS+ +P FSL+ ++ K LPI W S + S V
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFDRSAV 446
>SPAC343.11c |msc1||multi-copy suppressor of Chk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1588
Score = 26.2 bits (55), Expect = 1.5
Identities = 13/44 (29%), Positives = 19/44 (43%)
Frame = -3
Query: 230 SAFSLSNTQAYLKDPLPISWASFSNFSMVRFVNTTTFVDQVTSG 99
+AF S AY DP + + FS S +RF ++ G
Sbjct: 473 TAFPTSRQNAYYNDPWNLYFIHFSKLSPLRFTPPGILTSTISLG 516
>SPAC17G8.04c |arc15|arc5, arc5|ARP2/3 actin-organizing complex
subunit Arc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 152
Score = 25.4 bits (53), Expect = 2.6
Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = -3
Query: 266 QRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASFSN-FSMVRFVNTTTFVDQVTS 102
Q A+S +IP++RSA N LK L S+ + N VR FVD +++
Sbjct: 33 QAAVSQLIPQARSAIQTGNALQGLKTLL--SYVPYGNDVQEVRTQYLNAFVDVLSN 86
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 3.5
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 27 PKMGKEKTHINIVVIGHVDSGKST 98
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 25.0 bits (52), Expect = 3.5
Identities = 14/51 (27%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = +1
Query: 181 GKGSFKYAWVLDKLKAERERGITIDIALWK--FETSKYYVTIIDAPGHRDF 327
GK + A+ + + GIT I + F+ ++T +D PGH F
Sbjct: 183 GKTTLLDAFRKSTIASTEHGGITQKIGAFTVPFDKGSKFITFLDTPGHMAF 233
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.0 bits (52), Expect = 3.5
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 286 YYVTIIDAPGHRDF 327
Y + +ID+PGH DF
Sbjct: 94 YLINLIDSPGHVDF 107
Score = 23.8 bits (49), Expect = 8.0
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = +3
Query: 57 NIVVIGHVDSGKSTTTGHLIYKCGGIDKTYHREVR 161
N ++ HVD GK+T L+ G I VR
Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVR 55
>SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 96
Score = 24.6 bits (51), Expect = 4.6
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -2
Query: 345 CDHVLDEISVSRSINDGNIVLASFEL 268
CDH+L IS SR G + FEL
Sbjct: 23 CDHLLQYISNSRDSKAGLKIFLVFEL 48
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 24.2 bits (50), Expect = 6.1
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +3
Query: 60 IVVIGHVDSGKSTTTGHLIYKCGGIDKTYHR 152
++V+G SGK+T +Y+ G YHR
Sbjct: 511 LIVVGETGSGKTTQLAQFLYEDG-----YHR 536
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.2 bits (50), Expect = 6.1
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +3
Query: 60 IVVIGHVDSGKSTTTGHLIYKC 125
IVV+G SGKST + +C
Sbjct: 115 IVVVGETGSGKSTQIPQFLNEC 136
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 24.2 bits (50), Expect = 6.1
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +3
Query: 63 VVIGHVDSGKSTTTGHLIYKCG 128
+V+GH GK+T +Y+ G
Sbjct: 45 IVLGHTGCGKTTQIPQFLYEAG 66
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,367,339
Number of Sequences: 5004
Number of extensions: 24902
Number of successful extensions: 100
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 81
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 107972554
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -