Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32284
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 52 5e-08
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 47 1e-06
SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyce... 25 5.1
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 5.1
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 25 5.1
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.7
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 6.7
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 25 6.7
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 25 8.9
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 52.0 bits (119), Expect = 5e-08
Identities = 36/136 (26%), Positives = 61/136 (44%)
Frame = +2
Query: 80 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 259
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 260 KKDKDQGAYEDFLECLKLYDKNENGLMXXXXXXXXXXXXXXXXDDSEVAEVTKDCMDPED 439
G E+F++ +++DK+ G++ + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 440 DDGMIPYAAFLKKVMA 487
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 47.2 bits (107), Expect = 1e-06
Identities = 39/149 (26%), Positives = 63/149 (42%), Gaps = 3/149 (2%)
Frame = +2
Query: 44 TSAXNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI-XXXXXXXXXXX 214
T D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 3 TRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGN 62
Query: 215 XXXXXXXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMXXXXXXXXXXXXXXXXDD 394
FL + A+K KD E+ E K++DK+ NG +
Sbjct: 63 GTIDFTEFLTM--MARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQ 120
Query: 395 SEVAEVTKDCMDPEDDDGMIPYAAFLKKV 481
EVA++ ++ D DG+I Y F + +
Sbjct: 121 EEVADMIREA--DTDGDGVINYEEFSRVI 147
>SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 413
Score = 25.4 bits (53), Expect = 5.1
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = +3
Query: 18 PTNTTQNERPQQXTTLKGRLSPSQSTTLKAKVRSMPST 131
PTN+T ++ + + + R S QSTTL ST
Sbjct: 126 PTNSTTSQASAKHSAVPHRSSQFQSTTLTPSTTDSSST 163
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 5.1
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = +3
Query: 57 TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 230
T +PS L+A + S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 231 KSSFPSTAKQRKTKT 275
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 25.4 bits (53), Expect = 5.1
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 62 VERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 163
+E AF ++D + GKI NL + + LN N
Sbjct: 109 LEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 25.0 bits (52), Expect = 6.7
Identities = 19/78 (24%), Positives = 38/78 (48%)
Frame = +3
Query: 48 QQXTTLKGRLSPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSH 227
++ ++L +L+P T KAK S+ S S S+ + W + V++ A S
Sbjct: 345 REPSSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SP 401
Query: 228 SKSSFPSTAKQRKTKTRE 281
S++S P+ +K + ++
Sbjct: 402 SEASLPNLSKYSRKNAKK 419
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/22 (54%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
Frame = +2
Query: 59 DVER-ASFAFSIYDFEGKGKID 121
DV R SF F +YDF G G +D
Sbjct: 612 DVMRNISFIFELYDFNGDGFMD 633
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 25.0 bits (52), Expect = 6.7
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 3/46 (6%)
Frame = +3
Query: 15 EPTNTTQNERPQQXTTLKGR---LSPSQSTTLKAKVRSMPSTLAIS 143
+P + N P T K R ++PS ST A V + PS+ ++S
Sbjct: 593 QPVTSVLNTSPLPKTPEKDRSLNVTPSSSTPTPASVLAPPSSASLS 638
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 24.6 bits (51), Expect = 8.9
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = +1
Query: 457 LRSIPEESHGVEDITTRH 510
LR +P+E +G++D+ RH
Sbjct: 214 LRMLPKEHYGLKDLEIRH 231
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,891,848
Number of Sequences: 5004
Number of extensions: 32436
Number of successful extensions: 104
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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