Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV32265
         (516 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    71   1e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    71   1e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    57   1e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    32   0.059
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    27   1.7  
SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr...    27   2.2  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   3.8  
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe...    25   5.1  
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos...    25   8.9  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%)
 Frame = +2

Query: 35  MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 208
           M+  +  D + A F  AFS++D +  G I +  LG ++R+L  +PT A +          
Sbjct: 1   MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60

Query: 209 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 385
                    FL + ++  KD D    E+  E  K++DK+ NG +   ELTH L +LGE+L
Sbjct: 61  GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118

Query: 386 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 478
              EVA++ ++     D DG+I Y  F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 70.9 bits (166), Expect = 1e-13
 Identities = 44/136 (32%), Positives = 71/136 (52%)
 Frame = +2

Query: 77  AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 256
           AFS++D  G G+I   ++GDLLRA   NPTLA I                  FL + ++ 
Sbjct: 11  AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67

Query: 257 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 436
                 G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K       
Sbjct: 68  NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124

Query: 437 DDGMIPYAAFLKKVMA 484
            DGM+ Y  F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 57.2 bits (132), Expect = 1e-09
 Identities = 37/146 (25%), Positives = 71/146 (48%)
 Frame = +2

Query: 47  SKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 226
           SK   +    AF +YD +  G I   ++G +LR+L  N T A +                
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 227 XXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 406
             F+     + K ++  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  
Sbjct: 64  MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118

Query: 407 VTKDCMDPEDDDGMIPYAAFLKKVMA 484
           + ++  DP  + G   Y  F++++MA
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 31.9 bits (69), Expect = 0.059
 Identities = 33/161 (20%), Positives = 63/161 (39%), Gaps = 1/161 (0%)
 Frame = +2

Query: 2   RPGGNRQTPHKMSDLSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 181
           R GG      ++++  + D+  A   F ++D +    ID   L   +RAL  N   + + 
Sbjct: 20  RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76

Query: 182 XXXXXXXXXXXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 358
                             F+ + ++   ++D    E+     +L+D +E G +    L  
Sbjct: 77  KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134

Query: 359 TLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 481
               L E +DD E+  + ++     D DG I    F+  +M
Sbjct: 135 VAKELNENIDDQELEAMIEEF--DLDQDGEINEQEFIAIMM 173


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.1 bits (57), Expect = 1.7
 Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +2

Query: 35  MSDLSKNDVER-ASFAFSIYDFEGKGKID 118
           +++L   DV R  SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633


>SPBC215.06c |||human LYHRT homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 178

 Score = 26.6 bits (56), Expect = 2.2
 Identities = 19/92 (20%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
 Frame = +3

Query: 21  KHHTK*ATSARTTLKGRLSPSQSTTLKAKVR-SMPSTLAIS*ERSTQTPHWQPSRNSVVQ 197
           ++HT   T A+   KG   P++    KAK+  +  ++  +S     Q     P+++S+ +
Sbjct: 43  RNHTSCMTEAQRYQKGLYRPTKKELKKAKMNGNAVNSKELSPNTDNQNTPAGPTKHSLDE 102

Query: 198 RRRARSCSHSKSSFPSTAKQRKTKTRERMKIS 293
             + +    SK    S+  ++     +  +IS
Sbjct: 103 NEKDKENKKSKKETVSSPAEQLLALTQNQEIS 134


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 3.8
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +2

Query: 371 LGEKLDDSEVAEVTKDCMDPED 436
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 551

 Score = 25.4 bits (53), Expect = 5.1
 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
 Frame = +3

Query: 54  TTLKGRLSPSQSTTLKA-KVRSMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 227
           T      +PS    L+A  + S+P+ +L  S E +      + +R  ++  ++  S +  
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368

Query: 228 KSSFPSTAKQRKTKT 272
           KSSFPST K +   T
Sbjct: 369 KSSFPSTIKDQPNLT 383


>SPBC13G1.03c |pex14||peroxisomal membrane anchor
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 286

 Score = 24.6 bits (51), Expect = 8.9
 Identities = 17/48 (35%), Positives = 22/48 (45%)
 Frame = -1

Query: 174 VASVGFELSALRRSPRLKASILPLPSKS*IEKAKDALSTSFLLRSLIL 31
           V S GF  SA     +  A +   PS++  E  K+AL   FL    IL
Sbjct: 77  VISTGFAWSAYSLVKKYIAPMFRAPSQNAYEADKNALDAKFLEAHKIL 124


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,935,661
Number of Sequences: 5004
Number of extensions: 34960
Number of successful extensions: 117
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)