Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32247
(446 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 94 4e-20
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 86 9e-18
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 85 3e-17
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 65 2e-11
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 62 1e-10
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 43 8e-05
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 36 0.012
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 36 0.012
At2g47180.1 68415.m05892 galactinol synthase, putative similar t... 29 1.4
At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) fa... 29 1.9
At1g66270.2 68414.m07524 beta-glucosidase (PSR3.2) nearly identi... 29 1.9
At1g66270.1 68414.m07523 beta-glucosidase (PSR3.2) nearly identi... 29 1.9
At3g15690.2 68416.m01989 biotin carboxyl carrier protein of acet... 28 3.3
At3g15690.1 68416.m01988 biotin carboxyl carrier protein of acet... 28 3.3
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 27 4.4
At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP... 27 7.6
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 93.9 bits (223), Expect = 4e-20
Identities = 45/78 (57%), Positives = 55/78 (70%)
Frame = +2
Query: 212 STPQWTVXMRYYSSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKA 391
ST Q + S LP H+ + +PALSPTM G+I W KKEGDK+ GD++ EIETDKA
Sbjct: 195 STQQKSSIQPDASDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKA 254
Query: 392 TMGFETPEXGYLAKILIP 445
T+ FE+ E GYLAKILIP
Sbjct: 255 TLEFESLEEGYLAKILIP 272
Score = 85.8 bits (203), Expect = 1e-17
Identities = 37/55 (67%), Positives = 46/55 (83%)
Frame = +2
Query: 278 LPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKILI 442
+PALSPTM G++V W KKEGDK+ GD+LCEIETDKAT+ FE+ E G+LAKIL+
Sbjct: 90 MPALSPTMSHGNVVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKILV 144
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 86.2 bits (204), Expect = 9e-18
Identities = 37/64 (57%), Positives = 49/64 (76%)
Frame = +2
Query: 248 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEXGYL 427
S LP H ++ +P+LSPTM G+I W KKEGDK++ G++LCE+ETDKAT+ E E GYL
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYL 165
Query: 428 AKIL 439
AKI+
Sbjct: 166 AKIV 169
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 84.6 bits (200), Expect = 3e-17
Identities = 36/64 (56%), Positives = 49/64 (76%)
Frame = +2
Query: 248 SSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEXGYL 427
S LP H ++ +P+LSPTM G+I W KKEGDK++ G++LCE+ETDKAT+ E E G+L
Sbjct: 106 SDLPPHQEIGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFL 165
Query: 428 AKIL 439
AKI+
Sbjct: 166 AKIV 169
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 65.3 bits (152), Expect = 2e-11
Identities = 30/58 (51%), Positives = 41/58 (70%)
Frame = +2
Query: 269 KVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKILI 442
++ +PALS TM G IVSW K EGDKL++G+ + +E+DKA M ET GYLA I++
Sbjct: 41 EIFMPALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIMV 98
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 62.5 bits (145), Expect = 1e-10
Identities = 29/58 (50%), Positives = 41/58 (70%)
Frame = +2
Query: 269 KVNLPALSPTMESGSIVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKILI 442
++ +PALS TM G IVSW K EG+KL++G+ + +E+DKA M ET GYLA I++
Sbjct: 57 EIFMPALSSTMTEGKIVSWIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLAAIVV 114
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 43.2 bits (97), Expect = 8e-05
Identities = 20/86 (23%), Positives = 46/86 (53%), Gaps = 4/86 (4%)
Frame = +2
Query: 197 NQTVLSTPQWTVXMRYYSSLPSH----IKVNLPALSPTMESGSIVSWEKKEGDKLSEGDL 364
++T+++ Q T R+ S ++ +P + ++ G++ ++ KK GD++ +
Sbjct: 67 SRTIINGYQGTALQRWVRPFSSDSGDVVEAVVPHMGESITDGTLAAFLKKPGDRVEADEA 126
Query: 365 LCEIETDKATMGFETPEXGYLAKILI 442
+ +IETDK T+ +P G + + L+
Sbjct: 127 IAQIETDKVTIDIASPASGVIQEFLV 152
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 35.9 bits (79), Expect = 0.012
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +2
Query: 314 IVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKI 436
++ W KEGD + E LCE+++DKAT+ + G +A I
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALI 132
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 35.9 bits (79), Expect = 0.012
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +2
Query: 314 IVSWEKKEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKI 436
++ W KEGD + E LCE+++DKAT+ + G +A I
Sbjct: 92 LLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALI 132
>At2g47180.1 68415.m05892 galactinol synthase, putative similar to
galactinol synthase, isoform GolS-1 GI:5608497 from
[Ajuga reptans]
Length = 344
Score = 29.1 bits (62), Expect = 1.4
Identities = 18/62 (29%), Positives = 25/62 (40%)
Frame = +1
Query: 145 YRTGKKEXYE*IVRTCTEPDSSIYTTMDCXDEVLFXSTITYKGQPSSSFANHGEWFNC*L 324
Y G + YE I PD +Y MDC E + T YK + + +W L
Sbjct: 125 YLDGDIQVYENIDHLFDLPDGYLYAVMDCFCEKTWSHTPQYKIRYCQQCPDKVQWPKAEL 184
Query: 325 GK 330
G+
Sbjct: 185 GE 186
>At5g23110.1 68418.m02703 zinc finger (C3HC4-type RING finger) family
protein contains Pfam profile: PF00097 zinc finger, C3HC4
type (RING finger)
Length = 4706
Score = 28.7 bits (61), Expect = 1.9
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +2
Query: 266 IKVNLPALSPTMESGSIVSWEKKEGDKLSEG 358
++V L L P + G IV+W+ K+GDKL G
Sbjct: 4475 VQVQLHPLRPFYK-GEIVAWKIKQGDKLRYG 4504
>At1g66270.2 68414.m07524 beta-glucosidase (PSR3.2) nearly identical
to GI:2286069 from (Arabidopsis thaliana) (Plant Mol.
Biol. 34 (1), 57-68 (1997)); similar to thioglucoside
glucohydrolase (GI:984052) [Arabidopsis thaliana]
Length = 522
Score = 28.7 bits (61), Expect = 1.9
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +2
Query: 230 VXMRYYSSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDL 364
V + YY+S S+ P+ + S+VSWE K D + G +
Sbjct: 339 VGLNYYTSTFSNYNEKPDPSKPSWKQDSLVSWEPKNVDHSAIGSM 383
>At1g66270.1 68414.m07523 beta-glucosidase (PSR3.2) nearly identical
to GI:2286069 from (Arabidopsis thaliana) (Plant Mol.
Biol. 34 (1), 57-68 (1997)); similar to thioglucoside
glucohydrolase (GI:984052) [Arabidopsis thaliana]
Length = 524
Score = 28.7 bits (61), Expect = 1.9
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +2
Query: 230 VXMRYYSSLPSHIKVNLPALSPTMESGSIVSWEKKEGDKLSEGDL 364
V + YY+S S+ P+ + S+VSWE K D + G +
Sbjct: 341 VGLNYYTSTFSNYNEKPDPSKPSWKQDSLVSWEPKNVDHSAIGSM 385
>At3g15690.2 68416.m01989 biotin carboxyl carrier protein of
acetyl-CoA carboxylase-related contains weak similarity
to Biotin carboxyl carrier protein of acetyl-CoA
carboxylase, chloroplast precursor (BCCP)
(Swiss-Prot:Q42533) [Arabidopsis thaliana]
Length = 263
Score = 27.9 bits (59), Expect = 3.3
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 332 KEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKIL 439
KE D++ EG +LC IE E+ G + KIL
Sbjct: 201 KEKDQVKEGQILCYIEQLGGQFPIESDVTGEVVKIL 236
>At3g15690.1 68416.m01988 biotin carboxyl carrier protein of
acetyl-CoA carboxylase-related contains weak similarity
to Biotin carboxyl carrier protein of acetyl-CoA
carboxylase, chloroplast precursor (BCCP)
(Swiss-Prot:Q42533) [Arabidopsis thaliana]
Length = 247
Score = 27.9 bits (59), Expect = 3.3
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 332 KEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKIL 439
KE D++ EG +LC IE E+ G + KIL
Sbjct: 201 KEKDQVKEGQILCYIEQLGGQFPIESDVTGEVVKIL 236
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 27.5 bits (58), Expect = 4.4
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +2
Query: 332 KEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKIL 439
K GDK+ +G ++C IE K E + G + ++L
Sbjct: 202 KVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELL 237
>At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1)
identical to biotin carboxyl carrier protein of
acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
gi|9759121|dbj|BAB09606
Length = 280
Score = 26.6 bits (56), Expect = 7.6
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +2
Query: 332 KEGDKLSEGDLLCEIETDKATMGFETPEXGYLAKIL 439
K GDK+ +G +LC +E K E+ G + I+
Sbjct: 227 KVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIV 262
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,080,352
Number of Sequences: 28952
Number of extensions: 166873
Number of successful extensions: 436
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 429
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 436
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 722638680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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