Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32232
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g17890.1 68418.m02098 LIM domain-containing protein / disease... 31 0.35
At2g23140.1 68415.m02763 armadillo/beta-catenin repeat family pr... 31 0.61
At5g66610.1 68418.m08396 LIM domain-containing protein contains ... 30 1.1
At5g60250.1 68418.m07552 zinc finger (C3HC4-type RING finger) fa... 29 1.4
At3g09000.1 68416.m01053 proline-rich family protein 29 1.4
At4g36860.1 68417.m05226 LIM domain-containing protein low simil... 28 3.2
At5g52950.1 68418.m06570 expressed protein ; expression supporte... 28 4.3
At2g43690.1 68415.m05431 lectin protein kinase, putative similar... 28 4.3
At5g66630.1 68418.m08398 LIM domain-containing protein contains ... 27 7.5
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 27 7.5
At5g07350.1 68418.m00839 tudor domain-containing protein / nucle... 27 7.5
At4g33590.1 68417.m04772 hypothetical protein 27 7.5
At5g14890.1 68418.m01746 NHL repeat-containing protein contains ... 27 9.9
At4g39050.1 68417.m05531 kinesin-related protein (MKRP2) kinesin... 27 9.9
At3g57520.3 68416.m06405 alkaline alpha galactosidase, putative ... 27 9.9
At3g57520.2 68416.m06404 alkaline alpha galactosidase, putative ... 27 9.9
At3g57520.1 68416.m06403 alkaline alpha galactosidase, putative ... 27 9.9
At1g66680.1 68414.m07579 S locus-linked protein, putative simila... 27 9.9
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 27 9.9
>At5g17890.1 68418.m02098 LIM domain-containing protein / disease
resistance protein-related low similarity to disease
resistance protein RPP4 [Arabidopsis thaliana]
GI:20270890; contains Pfam profiles PF00412: LIM domain,
PF00931: NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1613
Score = 31.5 bits (68), Expect = 0.35
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +2
Query: 182 TRCKGCGNPIVDK-AIIALDAKWHRDCFTCMADRSNIRIVE 301
++CK C + I D +I A + WH CF C+ R I + E
Sbjct: 1238 SKCKDCKSAIEDGISINAYGSVWHPQCFCCLRCREPIAMNE 1278
>At2g23140.1 68415.m02763 armadillo/beta-catenin repeat family
protein / U-box domain-containing protein contains Pfam
domain, PF00514: Armadillo/beta-catenin-like repeats and
Pfam, PF04564: U-box domain
Length = 811
Score = 30.7 bits (66), Expect = 0.61
Identities = 28/109 (25%), Positives = 52/109 (47%), Gaps = 4/109 (3%)
Frame = +3
Query: 33 SLTV*SKLLACRGTLTTSSA-VDARRSLAVEGSWNRLAVPIAPTATQTSSP-LAARGAEI 206
SL S LL+C ++ ++ A V AR+ W+ ++++T P ++R E
Sbjct: 312 SLNELSPLLSCTDSIPSTGADVSARKVSNKSHDWDA-------SSSETGKPSFSSRATER 364
Query: 207 Q--SSIKPSSRLMLSGTAIASRAWRIEATFGSLREKVQESSDRFDILRD 347
+ S +P+S L S I+ + ++A GSL + S+D ++ D
Sbjct: 365 EGASPSRPASALGASSPGISGNGYGLDARRGSLNDFEDRSNDSRELRTD 413
>At5g66610.1 68418.m08396 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 529
Score = 29.9 bits (64), Expect = 1.1
Identities = 11/28 (39%), Positives = 16/28 (57%), Gaps = 1/28 (3%)
Frame = +2
Query: 188 CKGCGNPI-VDKAIIALDAKWHRDCFTC 268
C GC + I +++ AL WH +CF C
Sbjct: 201 CDGCKSAIEYGRSVHALGVNWHPECFCC 228
>At5g60250.1 68418.m07552 zinc finger (C3HC4-type RING finger)
family protein contains a Prosite:PS00518 Zinc finger,
C3HC4 type (RING finger), signature and Pfam domain
PF01485: IBR domain
Length = 655
Score = 29.5 bits (63), Expect = 1.4
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +2
Query: 188 CKGCGNPIVDKAIIALDAKWHRDCFTCMADRSNIRIV 298
C C N IV + + ++D HR CF C+ ++++
Sbjct: 301 CAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLL 337
>At3g09000.1 68416.m01053 proline-rich family protein
Length = 541
Score = 29.5 bits (63), Expect = 1.4
Identities = 23/76 (30%), Positives = 34/76 (44%)
Frame = +3
Query: 3 PAVPWXAATPSLTV*SKLLACRGTLTTSSAVDARRSLAVEGSWNRLAVPIAPTATQTSSP 182
PA P +T T S+ + R + + SS +R +L + A AP T TSS
Sbjct: 159 PATPTRRSTTPTTSTSRPVTTRASNSRSSTPTSRATLTAARATTSTA---APRTTTTSSG 215
Query: 183 LAARGAEIQSSIKPSS 230
A +S+ +PSS
Sbjct: 216 SARSATPTRSNPRPSS 231
>At4g36860.1 68417.m05226 LIM domain-containing protein low
similarity to LIM-domain protein [Branchiostoma
floridae] GI:3360516, DRAL/Slim3/FHL2 [Homo sapiens]
GI:7209525; contains Pfam profile PF00412: LIM domain
Length = 351
Score = 28.3 bits (60), Expect = 3.2
Identities = 13/50 (26%), Positives = 17/50 (34%)
Frame = +2
Query: 56 LGVSWHAHHFVXXXXXXXXXXXXFMEQAGRPYCSDCYADKFATRCKGCGN 205
+G WH F F RPY CY ++ +C C N
Sbjct: 1 MGGVWHPECFCCNACDKPIIDYEFSMSGNRPYHKLCYKEQHHPKCDVCHN 50
>At5g52950.1 68418.m06570 expressed protein ; expression supported
by MPSS
Length = 945
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/53 (26%), Positives = 23/53 (43%)
Frame = -3
Query: 265 REAIAVPLSIKRDDGFIDDWISAPLAASGELVCVAVGAIGTASLFHEPSTAKL 107
RE + +S +G + I P S L+CV + ++ HE S K+
Sbjct: 424 REGFSALVSFSNSNGSLRTGILKPFTFSSALICVFDNGVSPQTVDHEDSRKKV 476
>At2g43690.1 68415.m05431 lectin protein kinase, putative similar to
receptor-like kinase LECRK1 [Arabidopsis thaliana]
gi|2150023|gb|AAB58725
Length = 664
Score = 27.9 bits (59), Expect = 4.3
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = +3
Query: 288 FGSLREKVQESSDRFDILRDGKPATLR*MRLIHHKYINKIPHRDV 422
+G+ +K S RF I++D A + +HH +I+ + HRD+
Sbjct: 422 YGTSDQKQLSWSQRFKIIKDVASA----LSYLHHGWIHVVIHRDI 462
>At5g66630.1 68418.m08398 LIM domain-containing protein contains low
similarity to Pfam profile PF00412: LIM domain
Length = 702
Score = 27.1 bits (57), Expect = 7.5
Identities = 12/39 (30%), Positives = 19/39 (48%), Gaps = 1/39 (2%)
Frame = +2
Query: 188 CKGCGNPIV-DKAIIALDAKWHRDCFTCMADRSNIRIVE 301
C GC + + ++++ L WH CF C + I I E
Sbjct: 347 CGGCNSAVKHEESVNILGVLWHPGCFCCRSCDKPIAIHE 385
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 27.1 bits (57), Expect = 7.5
Identities = 18/58 (31%), Positives = 28/58 (48%)
Frame = +3
Query: 144 VPIAPTATQTSSPLAARGAEIQSSIKPSSRLMLSGTAIASRAWRIEATFGSLREKVQE 317
V + P+ T TS+P A+ SS P + ++GT S E F + +KVQ+
Sbjct: 153 VALPPSPTPTSTPATAKPTSAPSSSHPPLKSPMAGTFYRSPG-PGEPPFVKVGDKVQK 209
>At5g07350.1 68418.m00839 tudor domain-containing protein / nuclease
family protein contains Pfam domains PF00567: Tudor
domain and PF00565: Staphylococcal nuclease homologue
Length = 991
Score = 27.1 bits (57), Expect = 7.5
Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 4/54 (7%)
Frame = -2
Query: 203 FRTPCSEWRTCLRSSRSNRDGQP----VP*TLHRQAPSCIHRRRSGERATTRQE 54
F +P +E R CL S RS + G P P R+A + +R G++ + E
Sbjct: 402 FGSPAAERRVCLSSIRSPKMGNPRREEKPAPYAREAREFLRQRLIGKQVIVQME 455
>At4g33590.1 68417.m04772 hypothetical protein
Length = 466
Score = 27.1 bits (57), Expect = 7.5
Identities = 9/25 (36%), Positives = 17/25 (68%), Gaps = 2/25 (8%)
Frame = -1
Query: 363 EWLVFHHGECR--IGHWIPALFLST 295
+W+++H GE R +GHW+ + +T
Sbjct: 199 KWVLYHWGELRFGMGHWLSEIVTAT 223
>At5g14890.1 68418.m01746 NHL repeat-containing protein contains
Pfam profile PF01436: NHL repeat
Length = 754
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = -1
Query: 312 ALFLSTIRMLLLSAMHVKQSRCHLASSAMMALSTIGFPHPLQR 184
AL + +R L S+ H +S+ H+A+ +M +P P+++
Sbjct: 266 ALLVRRVRSLFSSSSHDTKSKRHVATPSMTMAPYQRYPRPVRQ 308
>At4g39050.1 68417.m05531 kinesin-related protein (MKRP2) kinesin
motor protein - Ustilago maydis, PID:g2062750; identical
to cDNA MKRP2 mRNA for kinesin-related protein
GI:16902293, kinesin-related protein [Arabidopsis
thaliana] GI:16902294
Length = 1055
Score = 26.6 bits (56), Expect = 9.9
Identities = 20/60 (33%), Positives = 30/60 (50%)
Frame = +3
Query: 111 LAVEGSWNRLAVPIAPTATQTSSPLAARGAEIQSSIKPSSRLMLSGTAIASRAWRIEATF 290
+A S R + P +P ++ +SS L+ R +S SS L+ S IASR+ TF
Sbjct: 1 MASSSSRTRSSRPPSPASSTSSSHLSNRLIPRSNSTSASS-LITSAAGIASRSMTPSRTF 59
>At3g57520.3 68416.m06405 alkaline alpha galactosidase, putative
similar to alkaline alpha galactosidase II [Cucumis
melo] GI:29838631; contains Pfam profile PF05691:
Raffinose synthase or seed imbibition protein Sip1
Length = 565
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +3
Query: 276 IEATFGSLREKVQESSDRFDILRDGKPATLR*MRLIHHKYINKIP 410
+E + G+ V ++ F+++R A R M+ HH+ K+P
Sbjct: 150 VETSQGTHLVYVHAGTNPFEVIRQSVKAVERHMQTFHHREKKKLP 194
>At3g57520.2 68416.m06404 alkaline alpha galactosidase, putative
similar to alkaline alpha galactosidase II [Cucumis
melo] GI:29838631; contains Pfam profile PF05691:
Raffinose synthase or seed imbibition protein Sip1
Length = 656
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +3
Query: 276 IEATFGSLREKVQESSDRFDILRDGKPATLR*MRLIHHKYINKIP 410
+E + G+ V ++ F+++R A R M+ HH+ K+P
Sbjct: 150 VETSQGTHLVYVHAGTNPFEVIRQSVKAVERHMQTFHHREKKKLP 194
>At3g57520.1 68416.m06403 alkaline alpha galactosidase, putative
similar to alkaline alpha galactosidase II [Cucumis
melo] GI:29838631; contains Pfam profile PF05691:
Raffinose synthase or seed imbibition protein Sip1
Length = 773
Score = 26.6 bits (56), Expect = 9.9
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +3
Query: 276 IEATFGSLREKVQESSDRFDILRDGKPATLR*MRLIHHKYINKIP 410
+E + G+ V ++ F+++R A R M+ HH+ K+P
Sbjct: 150 VETSQGTHLVYVHAGTNPFEVIRQSVKAVERHMQTFHHREKKKLP 194
>At1g66680.1 68414.m07579 S locus-linked protein, putative similar
to S locus-linked protein SLL2 [Brassica napus]
GI:1518113
Length = 358
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/66 (22%), Positives = 30/66 (45%)
Frame = +3
Query: 144 VPIAPTATQTSSPLAARGAEIQSSIKPSSRLMLSGTAIASRAWRIEATFGSLREKVQESS 323
+ + P +T+ AR A ++ S +IA+ +W I++ +GS + Q +
Sbjct: 4 IRLLPEEPETTPQQQARAAAAVTTTTTDSLASDDDRSIAADSWSIKSEYGSTLDDDQRHA 63
Query: 324 DRFDIL 341
D + L
Sbjct: 64 DAAEAL 69
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 26.6 bits (56), Expect = 9.9
Identities = 13/44 (29%), Positives = 19/44 (43%), Gaps = 1/44 (2%)
Frame = +2
Query: 188 CKGCGNPIVDKAII-ALDAKWHRDCFTCMADRSNIRIVERKSAG 316
C GC I + L++ WH +CF C I E ++G
Sbjct: 172 CAGCNMEIGHGRFLNCLNSLWHPECFRCYGCSQPISEYEFSTSG 215
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,132,416
Number of Sequences: 28952
Number of extensions: 220358
Number of successful extensions: 632
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 621
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 632
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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