Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV32218
         (516 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g19820.1 68418.m02355 PBS lyase HEAT-like repeat-containing p...    77   5e-15
At4g27640.1 68417.m03973 importin beta-2 subunit family protein ...    63   1e-10
At2g47230.1 68415.m05898 agenet domain-containing protein contai...    36   0.012
At5g57020.1 68418.m07117 myristoyl-CoA:protein N-myristoyltransf...    30   1.1  
At1g26170.1 68414.m03194 importin beta-2 subunit family protein ...    29   1.4  
At5g16000.1 68418.m01871 leucine-rich repeat family protein / pr...    28   3.2  
At2g17780.1 68415.m02059 expressed protein contains Pfam profile...    27   7.5  
At2g04660.1 68415.m00475 E3 ubiquitin ligase, putative E3, ubiqu...    27   7.5  
At1g15360.1 68414.m01839 AP2 domain-containing transcription fac...    27   7.5  
At3g44180.1 68416.m04737 syntaxin-related family protein contain...    27   9.9  
At2g43980.1 68415.m05469 inositol 1,3,4-trisphosphate 5/6-kinase...    27   9.9  

>At5g19820.1 68418.m02355 PBS lyase HEAT-like repeat-containing
           protein contains Pfam profile: PF03130 PBS lyase
           HEAT-like repeat
          Length = 1116

 Score = 77.4 bits (182), Expect = 5e-15
 Identities = 48/167 (28%), Positives = 84/167 (50%), Gaps = 3/167 (1%)
 Frame = +3

Query: 3   DQAQFYQLLNTLLSTDNDIRSQAEDAYN---NIPTETKVVHLVNSIQNADIAEDVRQTAA 173
           D A F  L++ L+S+ N+ RS AE  +N       +T  + L + +Q +   E  R  AA
Sbjct: 20  DSAPFETLISHLMSSSNEQRSSAESLFNLAKQSNPDTLSLKLAHLLQLSPHPEG-RAMAA 78

Query: 174 VLLRRLFSAXXXXXXXXXXXXQQTVLREQLLLTLQMDLSQYLRRKVCDVVSELARNHIDD 353
           VLLR+L +              Q+ L+  +L  +Q + ++ + +K+CD VSELA   + +
Sbjct: 79  VLLRKLLTRDDAYLWPRLSLSTQSSLKSSMLYCIQHEEAKSISKKICDTVSELASGILPE 138

Query: 354 DGNNQWPEFLQFMFTCASAQDPNIKEAGIRMFTSVPGVFGNRQTENL 494
           +G   WPE L F+F C ++  P ++E+   +   +    G   T ++
Sbjct: 139 NG---WPELLPFVFQCVTSVTPKLQESAFLILAQLSQYVGETLTPHI 182


>At4g27640.1 68417.m03973 importin beta-2 subunit family protein low
           similarity to importin 4 GI:18700635 from [Homo sapiens]
          Length = 1048

 Score = 62.9 bits (146), Expect = 1e-10
 Identities = 46/152 (30%), Positives = 78/152 (51%), Gaps = 1/152 (0%)
 Frame = +3

Query: 24  LLNTLLSTDNDIRSQAEDAYNNIPTETKVVH-LVNSIQNADIAEDVRQTAAVLLRRLFSA 200
           LL   L  DND R QAED    +  + +VV  LV  ++ A    +VRQ AAVLLR+  + 
Sbjct: 8   LLIQFLMPDNDARRQAEDQIKRLAKDPQVVPALVQHLRTAK-TPNVRQLAAVLLRKRITG 66

Query: 201 XXXXXXXXXXXXQQTVLREQLLLTLQMDLSQYLRRKVCDVVSELARNHIDDDGNNQWPEF 380
                       +Q V ++ L+ ++ ++ S  +RR   +VVS +A+  +      +WP+ 
Sbjct: 67  HWAKLSPQL---KQHV-KQSLIESITVENSPPVRRASANVVSVVAKYAVPA---GEWPDL 119

Query: 381 LQFMFTCASAQDPNIKEAGIRMFTSVPGVFGN 476
           L F+F C+ +   + +E  + +F+S+    GN
Sbjct: 120 LTFLFQCSQSAQEDHREVALILFSSLTETIGN 151


>At2g47230.1 68415.m05898 agenet domain-containing protein contains
           Pfam PF05641: Agenet domain
          Length = 701

 Score = 36.3 bits (80), Expect = 0.012
 Identities = 19/49 (38%), Positives = 26/49 (53%)
 Frame = +3

Query: 27  LNTLLSTDNDIRSQAEDAYNNIPTETKVVHLVNSIQNADIAEDVRQTAA 173
           +N   S  ND+  Q   A+ N+PTET + H    + NA IA DV +  A
Sbjct: 459 INDTSSICNDVDDQPLAAWINLPTETSIDHSPIVVNNAAIATDVEERQA 507


>At5g57020.1 68418.m07117 myristoyl-CoA:protein
           N-myristoyltransferase 1 (NMT1) identical to
           N-myristoyltransferase 1 (NMT1) [Arabidopsis thaliana]
           GI:7339834
          Length = 434

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +3

Query: 3   DQAQFYQLLNTLLSTDNDIRSQAEDAYNNIPTETKVVHLVN 125
           D   FY L +T+L   N    +A  +Y N+ T+T  + L+N
Sbjct: 333 DFCSFYTLPSTILGNPNYTTLKAAYSYYNVATQTSFLQLMN 373


>At1g26170.1 68414.m03194 importin beta-2 subunit family protein
           similar to Importin9 isoform 1 [Mus musculus]
           GI:15186756; contains Pfam profile PF03810:
           Importin-beta N-terminal domain
          Length = 931

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 34/173 (19%), Positives = 63/173 (36%), Gaps = 5/173 (2%)
 Frame = +3

Query: 3   DQAQFYQLLNTLLSTDNDIRSQAEDAYNNIPTETKVVH-LVNSIQNADIAEDVRQ---TA 170
           DQ      L+  L  + ++RS AE + N    +      L     N D++  +RQ   ++
Sbjct: 6   DQQWLLGCLSASLDPNQNVRSFAETSLNQASLQPGFGSALCRVAANKDLSLGLRQISFSS 65

Query: 171 AVLLRRLFSAXXXXXXXXXXXXQQTVLREQLLLTLQMDLSQYLRRKVCDVVSELARNHID 350
           AVLL++                  +   + L+    +       RK+C  +S    +   
Sbjct: 66  AVLLKQFIKKHWRENEEAFEYPLVSSEEKALIRGQLLGSLDDSHRKICTAISMDISSIAT 125

Query: 351 DDGNNQWPEFLQFMFTCAS-AQDPNIKEAGIRMFTSVPGVFGNRQTENLDVIK 506
            D   +WPE + F+    S   + N     +R    + G   +++   L   K
Sbjct: 126 YDWPEEWPELVPFLLKLISDPSNTNGVHGALRCLALLSGELDDKEVPTLSYDK 178


>At5g16000.1 68418.m01871 leucine-rich repeat family protein /
           protein kinase family protein contains Pfam domains
           PF00560: Leucine Rich Repeat and PF00069: Protein kinase
           domain
          Length = 638

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 7/26 (26%), Positives = 17/26 (65%)
 Frame = -2

Query: 497 IQVFSLTISKYTWYRRKHSNTSFFDV 420
           + +  + +  + W+R++H+  +FFDV
Sbjct: 258 VSLIFIAVGLFLWWRQRHNQNTFFDV 283


>At2g17780.1 68415.m02059 expressed protein contains Pfam profile
           PF04749: Protein of unknown function, DUF614
          Length = 416

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 16/39 (41%), Positives = 21/39 (53%)
 Frame = +3

Query: 36  LLSTDNDIRSQAEDAYNNIPTETKVVHLVNSIQNADIAE 152
           LL+   +I +Q E A N I    K+V L+N   NA I E
Sbjct: 100 LLAMGWNIVNQFEKAQNEIDLFLKIVPLINMADNARIRE 138


>At2g04660.1 68415.m00475 E3 ubiquitin ligase, putative E3,
           ubiquitin ligase; contains similarity to
           anaphase-promoting complex subunit 2 GI:6180009 from
           [Homo sapiens]
          Length = 865

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 22/65 (33%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
 Frame = +1

Query: 268 SLFKWIFPSTYEERFVM*SLNWLGIILMTMATTSGLSSYSSCLPVP--VRKIQTSKKLVL 441
           S+ +WI   T   +F+   L++LG  +    T+SGL+S  +C P P   R +  S+ +V 
Sbjct: 290 SIKEWI--QTVPLQFLNALLSYLGDSVSYGTTSSGLTSPLACCPSPSFSRVVTPSEGIVR 347

Query: 442 ECLRL 456
             LRL
Sbjct: 348 WKLRL 352


>At1g15360.1 68414.m01839 AP2 domain-containing transcription factor
           family protein Similar to SP|P16146 PPLZ02 protein
           {Lupinus polyphyllus}; contains an PF|00847 AP2 domain.
           EST gb|AA728476 comes from this gene
          Length = 199

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 15/35 (42%), Positives = 20/35 (57%)
 Frame = +1

Query: 352 TMATTSGLSSYSSCLPVPVRKIQTSKKLVLECLRL 456
           TM++++  SS SS L   +RK   S    L CLRL
Sbjct: 84  TMSSSTSSSSLSSILSAKLRKCCKSPSPSLTCLRL 118


>At3g44180.1 68416.m04737 syntaxin-related family protein contains a
           novel domain similar to F-box that is shared among other
           proteins in Arabidopsis; similar to proteins At3g54160,
           At1g47920 (syntaxin SYP81), At5g41830, At3g58890,
           At1g56610, At1g48390,  At3g59270 [Arabidopsis thaliana]
          Length = 202

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 12/42 (28%), Positives = 22/42 (52%)
 Frame = +1

Query: 346 LMTMATTSGLSSYSSCLPVPVRKIQTSKKLVLECLRLYQVYL 471
           L+ +  +S    +   LP+ + +    K LVL CL + +VY+
Sbjct: 130 LVDLVFSSKKEGWKVLLPLLIERSPNLKNLVLSCLEVVKVYV 171


>At2g43980.1 68415.m05469 inositol 1,3,4-trisphosphate 5/6-kinase
           family protein contains Pfam profile PF05770: Inositol
           1, 3, 4-trisphosphate 5/6-kinase;  contains Prosite
           PS00591: Glycosyl hydrolases family 10 active site;
           similar to inositol 1,3,4-trisphosphate 5/6-kinase
           (GI:1322038) [Homo sapiens]
          Length = 488

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 15/34 (44%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -2

Query: 464 TWYRRKHSNTSF-FDVWILRTGTGKHEL*ELRPL 366
           TW R+K   T F FDV +++ GTG H + +L  L
Sbjct: 425 TWLRKKLDLTIFGFDV-VIQEGTGDHVIVDLNYL 457


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,699,994
Number of Sequences: 28952
Number of extensions: 204224
Number of successful extensions: 565
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 560
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 562
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)