Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32216
(516 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P36188 Cluster: Troponin I; n=50; cellular organisms|Re... 81 1e-14
UniRef50_UPI0000E48440 Cluster: PREDICTED: similar to troponin I... 45 0.001
UniRef50_Q9XUN9 Cluster: Troponin I 3; n=6; Caenorhabditis|Rep: ... 40 0.025
UniRef50_Q7M3Y3 Cluster: Troponin I; n=5; Pectinidae|Rep: Tropon... 39 0.078
UniRef50_Q8WPC1 Cluster: Adult cardiac troponin I; n=9; Ascidiac... 37 0.31
UniRef50_Q5DDC3 Cluster: SJCHGC00516 protein; n=4; Schistosoma j... 36 0.72
UniRef50_Q4RGJ7 Cluster: Chromosome undetermined SCAF15099, whol... 35 1.3
UniRef50_P45378 Cluster: Troponin T, fast skeletal muscle; n=64;... 33 5.1
UniRef50_P45379 Cluster: Troponin T, cardiac muscle; n=294; Eute... 33 5.1
UniRef50_Q1UAM1 Cluster: Putative uncharacterized protein; n=1; ... 32 6.8
UniRef50_A4MI06 Cluster: Putative uncharacterized protein; n=1; ... 32 6.8
UniRef50_Q9NDR9 Cluster: Troponin I; n=1; Branchiostoma belcheri... 32 8.9
UniRef50_Q6AHQ1 Cluster: Troponin t protein 3, isoform b; n=9; R... 32 8.9
UniRef50_Q4XS40 Cluster: Putative uncharacterized protein; n=3; ... 32 8.9
UniRef50_Q12019 Cluster: Midasin; n=4; Saccharomycetaceae|Rep: M... 32 8.9
>UniRef50_P36188 Cluster: Troponin I; n=50; cellular organisms|Rep:
Troponin I - Drosophila melanogaster (Fruit fly)
Length = 269
Score = 81.4 bits (192), Expect = 1e-14
Identities = 42/112 (37%), Positives = 57/112 (50%)
Frame = +2
Query: 170 IDRKRAEVRKRMEEASXXXXXXXGFMTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 349
I+RKRAEVRKRMEEAS GFMTP
Sbjct: 75 IERKRAEVRKRMEEASKAKKAKKGFMTPERKKKLRLLLRKKAAEELKKEQERKAAERRRI 134
Query: 350 XXXXCGKPKNIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEIS 505
CG P+N+ DA+E ++ +C++Y+ER+ E K+DLEY V++KD EI+
Sbjct: 135 IEERCGSPRNLSDASEGELQEICEEYYERMYICEGQKWDLEYEVRKKDWEIN 186
>UniRef50_UPI0000E48440 Cluster: PREDICTED: similar to troponin I,
partial; n=1; Strongylocentrotus purpuratus|Rep:
PREDICTED: similar to troponin I, partial -
Strongylocentrotus purpuratus
Length = 312
Score = 44.8 bits (101), Expect = 0.001
Identities = 17/45 (37%), Positives = 31/45 (68%)
Frame = +2
Query: 368 KPKNIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEI 502
KP +ID + D ++ +C++ E++ E KFDLE I++++D+EI
Sbjct: 230 KPLSIDSLSRDRLRALCEELTEQLTDAEGNKFDLEVIIRKQDLEI 274
>UniRef50_Q9XUN9 Cluster: Troponin I 3; n=6; Caenorhabditis|Rep:
Troponin I 3 - Caenorhabditis elegans
Length = 260
Score = 40.3 bits (90), Expect = 0.025
Identities = 27/110 (24%), Positives = 44/110 (40%), Gaps = 1/110 (0%)
Frame = +2
Query: 179 KRAEVRKRMEEASXXXXXXXGFMTPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 358
K+AEVRKRMEEA+ GF+TP
Sbjct: 31 KKAEVRKRMEEAAKKGSKKKGFLTPERKKKLRKLLMMKAAEDLKQQQMLKEQERQKTLQQ 90
Query: 359 XCGKPKNIDDANED-TIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEIS 505
++D N+ + ++ +D R+ LE+ KFD+ + V + + EI+
Sbjct: 91 RTIPLPDVDSINDQGQLLKIYEDMFARVCALEEEKFDINFGVSQTEAEIN 140
>UniRef50_Q7M3Y3 Cluster: Troponin I; n=5; Pectinidae|Rep: Troponin
I - Chlamys nipponensis akazara (Akazara scallop)
(Japanese scallop)
Length = 292
Score = 38.7 bits (86), Expect = 0.078
Identities = 16/46 (34%), Positives = 31/46 (67%), Gaps = 1/46 (2%)
Frame = +2
Query: 371 PK-NIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEIS 505
PK + D + ++ +CKD+H+R+A LE+ +D E ++++D EI+
Sbjct: 184 PKFSTDGKDVAALQALCKDFHKRLASLEEDVYDWEAKIRKQDFEIN 229
>UniRef50_Q8WPC1 Cluster: Adult cardiac troponin I; n=9;
Ascidiacea|Rep: Adult cardiac troponin I - Halocynthia
roretzi (Sea squirt)
Length = 260
Score = 36.7 bits (81), Expect = 0.31
Identities = 12/42 (28%), Positives = 27/42 (64%)
Frame = +2
Query: 377 NIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEI 502
N+ +E +K +C++ H +I ++++ ++D+E V + D EI
Sbjct: 132 NLGGMSEQDLKDLCRELHAKIEKVDEQRYDIEVKVNKNDQEI 173
>UniRef50_Q5DDC3 Cluster: SJCHGC00516 protein; n=4; Schistosoma
japonicum|Rep: SJCHGC00516 protein - Schistosoma
japonicum (Blood fluke)
Length = 221
Score = 35.5 bits (78), Expect = 0.72
Identities = 15/46 (32%), Positives = 27/46 (58%)
Frame = +2
Query: 368 KPKNIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEIS 505
+P +D E+ + K +ER+ +LE K+D E ++R+D EI+
Sbjct: 108 EPLKLDGLGENDLNAKVKQLYERVRQLEGDKYDWEEKLRRQDFEIN 153
>UniRef50_Q4RGJ7 Cluster: Chromosome undetermined SCAF15099, whole
genome shotgun sequence; n=2; Tetraodontidae|Rep:
Chromosome undetermined SCAF15099, whole genome shotgun
sequence - Tetraodon nigroviridis (Green puffer)
Length = 334
Score = 34.7 bits (76), Expect = 1.3
Identities = 15/45 (33%), Positives = 27/45 (60%)
Frame = +2
Query: 368 KPKNIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEI 502
KP N+D+ ++D ++ K+ E + LE+ KFD +KR+ E+
Sbjct: 262 KPLNVDNLSDDKLRETAKELWELLYNLEEIKFDHCEKLKRQRYEV 306
>UniRef50_P45378 Cluster: Troponin T, fast skeletal muscle; n=64;
Euteleostomi|Rep: Troponin T, fast skeletal muscle -
Homo sapiens (Human)
Length = 269
Score = 32.7 bits (71), Expect = 5.1
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +2
Query: 368 KPKNIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEIS 505
KP NID ED ++ K+ E + +LE KF+ +KR+ +I+
Sbjct: 195 KPLNIDHLGEDKLRDKAKELWETLHQLEIDKFEFGEKLKRQKYDIT 240
>UniRef50_P45379 Cluster: Troponin T, cardiac muscle; n=294;
Euteleostomi|Rep: Troponin T, cardiac muscle - Homo
sapiens (Human)
Length = 298
Score = 32.7 bits (71), Expect = 5.1
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +2
Query: 380 IDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEIS 505
ID NED ++ K+ + I LE KFDL+ K++ EI+
Sbjct: 231 IDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEIN 272
>UniRef50_Q1UAM1 Cluster: Putative uncharacterized protein; n=1;
Lactobacillus reuteri 100-23|Rep: Putative
uncharacterized protein - Lactobacillus reuteri 100-23
Length = 228
Score = 32.3 bits (70), Expect = 6.8
Identities = 14/50 (28%), Positives = 30/50 (60%)
Frame = +2
Query: 365 GKPKNIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEISPPE 514
G +N D ++ + +C+ +H+ I RL + +F+ +Y + K +++ PPE
Sbjct: 167 GNNRNKIDHSKRELYPLCRFHHQEIERLNNKRFEAKYHIHVKGLKL-PPE 215
>UniRef50_A4MI06 Cluster: Putative uncharacterized protein; n=1;
Geobacter bemidjiensis Bem|Rep: Putative uncharacterized
protein - Geobacter bemidjiensis Bem
Length = 404
Score = 32.3 bits (70), Expect = 6.8
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +2
Query: 365 GKPKNIDDANEDTIKRVCKDYHERIARL 448
G+P +D+ + +KRVC+DY E + R+
Sbjct: 158 GRPYALDEQRDLVLKRVCRDYEEAVNRI 185
>UniRef50_Q9NDR9 Cluster: Troponin I; n=1; Branchiostoma
belcheri|Rep: Troponin I - Branchiostoma belcheri
(Amphioxus)
Length = 180
Score = 31.9 bits (69), Expect = 8.9
Identities = 12/45 (26%), Positives = 25/45 (55%)
Frame = +2
Query: 371 PKNIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEIS 505
P + D ++ +C+ H +I + E+ K+D+E V+ D E++
Sbjct: 50 PISTSGLGHDELEELCRRLHAQITKAEEEKYDVEQKVEINDKELA 94
>UniRef50_Q6AHQ1 Cluster: Troponin t protein 3, isoform b; n=9;
Rhabditida|Rep: Troponin t protein 3, isoform b -
Caenorhabditis elegans
Length = 1178
Score = 31.9 bits (69), Expect = 8.9
Identities = 13/42 (30%), Positives = 25/42 (59%)
Frame = +2
Query: 377 NIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEI 502
N + + +K K+ H+RI +LE K+DLE +R++ ++
Sbjct: 1043 NASEVPQSELKAKIKELHQRICKLETEKYDLEKRHERQEYDM 1084
>UniRef50_Q4XS40 Cluster: Putative uncharacterized protein; n=3;
Plasmodium (Vinckeia)|Rep: Putative uncharacterized
protein - Plasmodium chabaudi
Length = 467
Score = 31.9 bits (69), Expect = 8.9
Identities = 13/42 (30%), Positives = 25/42 (59%)
Frame = +2
Query: 377 NIDDANEDTIKRVCKDYHERIARLEDXKFDLEYIVKRKDMEI 502
N+ + N +K + KDY+E + +D +FD+ ++R D+ I
Sbjct: 309 NLYNENYIKMKELLKDYNEAVKNKKDTEFDMNVFIERYDIII 350
>UniRef50_Q12019 Cluster: Midasin; n=4; Saccharomycetaceae|Rep:
Midasin - Saccharomyces cerevisiae (Baker's yeast)
Length = 4910
Score = 31.9 bits (69), Expect = 8.9
Identities = 22/64 (34%), Positives = 31/64 (48%)
Frame = +3
Query: 321 NVKLLKGGASSRRGAVNLRTLTTPTKILLRGFAKTTMNASLVLKMXNSIWNTSLKGKIWR 500
NV LK SSR+ NL + + LL G AKT + A L+ N + +LK +
Sbjct: 3517 NVDALK--QSSRKSHNNLYKIVRKYRDLLNGDAKTIIEAGLLYSNENKLKLPTLKQHFYE 3574
Query: 501 SPHL 512
P+L
Sbjct: 3575 DPNL 3578
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 370,383,117
Number of Sequences: 1657284
Number of extensions: 5059529
Number of successful extensions: 14538
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 14228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14536
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 31782822356
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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