Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32215
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 169 1e-42
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 122 1e-28
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 122 1e-28
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 120 8e-28
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 89 2e-18
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 52 3e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 52 3e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 3e-06
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 3e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 3e-04
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 31 0.61
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 1.9
At2g35490.1 68415.m04347 plastid-lipid associated protein PAP, p... 28 4.3
At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /... 27 5.7
At3g63180.1 68416.m07097 expressed protein 27 5.7
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 27 7.5
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 169 bits (410), Expect = 1e-42
Identities = 74/120 (61%), Positives = 96/120 (80%)
Frame = +2
Query: 2 LYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYL 181
+YA +TA+PRL+EPVY+ EIQ PE A+GGIY VLN++RGHVFEE Q GTP++ +KAYL
Sbjct: 714 IYASQITAKPRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYL 773
Query: 182 PVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGL 361
PV ESFGF++ LR+ T GQAFPQCVFDHW+++ DP EP ++ +V + RKRKGLKE +
Sbjct: 774 PVVESFGFSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPGTQASVLVADIRKRKGLKEAM 833
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 122 bits (294), Expect = 1e-28
Identities = 66/129 (51%), Positives = 80/129 (62%), Gaps = 12/129 (9%)
Frame = +2
Query: 5 YACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLP 184
Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LP
Sbjct: 831 YSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLP 890
Query: 185 VNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQE 328
V ESFGF DLR +T GQAF VFDHW ++PGDP + Q +P + +
Sbjct: 891 VIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVK 950
Query: 329 TRKRKGLKE 355
TR+RKG+ E
Sbjct: 951 TRRRKGMSE 959
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 122 bits (294), Expect = 1e-28
Identities = 66/129 (51%), Positives = 80/129 (62%), Gaps = 12/129 (9%)
Frame = +2
Query: 5 YACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLP 184
Y+ L A PRLMEPVY EIQ P V IY VL+RRRGHV + GTP +IVKA+LP
Sbjct: 831 YSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLP 890
Query: 185 VNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQE 328
V ESFGF DLR +T GQAF VFDHW ++PGDP + Q +P + +
Sbjct: 891 VIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVK 950
Query: 329 TRKRKGLKE 355
TR+RKG+ E
Sbjct: 951 TRRRKGMSE 959
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 120 bits (288), Expect = 8e-28
Identities = 65/129 (50%), Positives = 80/129 (62%), Gaps = 12/129 (9%)
Frame = +2
Query: 5 YACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLP 184
Y+ L A PRLMEPVY EIQ P V IY VL+RRRG+V + GTP +IVKA+LP
Sbjct: 817 YSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLP 876
Query: 185 VNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEP--QSKPY----------NVVQE 328
V ESFGF DLR +T GQAF VFDHW ++PGDP + Q +P + +
Sbjct: 877 VIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVK 936
Query: 329 TRKRKGLKE 355
TR+RKG+ E
Sbjct: 937 TRRRKGMSE 945
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 88.6 bits (210), Expect = 2e-18
Identities = 39/94 (41%), Positives = 56/94 (59%)
Frame = +2
Query: 8 ACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPV 187
A +L PR++E +Y CE+ +G +Y VL+RRR + +E G+ +F V AY+PV
Sbjct: 865 AAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVHAYVPV 924
Query: 188 NESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 289
+ESFGF +LR T G A V HW++L DP
Sbjct: 925 SESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 51.6 bits (118), Expect = 3e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = +2
Query: 11 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 190
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 191 ESFGFTADLRSNTGGQ 238
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 51.6 bits (118), Expect = 3e-07
Identities = 25/76 (32%), Positives = 42/76 (55%)
Frame = +2
Query: 11 CLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVN 190
C A+P ++EPV L E++ P G + G +N+R+G + Q ++ A +P+N
Sbjct: 649 CYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLN 706
Query: 191 ESFGFTADLRSNTGGQ 238
FG++ LRS T G+
Sbjct: 707 NMFGYSTSLRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 48.4 bits (110), Expect = 3e-06
Identities = 27/86 (31%), Positives = 44/86 (51%)
Frame = +2
Query: 23 AQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFG 202
A PR++EP+ E+ PE +G + G LN RRG + G + +V + +P+ E F
Sbjct: 683 AGPRMLEPIMRVEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQ 741
Query: 203 FTADLRSNTGGQAFPQCVFDHWQVLP 280
+ + LR T G+A + V+P
Sbjct: 742 YVSTLRGMTKGRASYTMQLAKFDVVP 767
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 41.5 bits (93), Expect = 3e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = +2
Query: 32 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 211
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 473 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 532
Query: 212 DLRSNTGGQAFPQCVFDHWQVLPGD 286
+ + + G A VFD + GD
Sbjct: 533 AILTASRGTAILNTVFDSYGPWAGD 557
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 41.5 bits (93), Expect = 3e-04
Identities = 21/85 (24%), Positives = 39/85 (45%)
Frame = +2
Query: 32 RLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTA 211
+L+EP + ++ PE +G + +L +RRG +F+ V ++ +P G
Sbjct: 472 KLLEPYEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRN 531
Query: 212 DLRSNTGGQAFPQCVFDHWQVLPGD 286
+ + + G A VFD + GD
Sbjct: 532 AILTASRGTAILNTVFDSYGPWAGD 556
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 30.7 bits (66), Expect = 0.61
Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%)
Frame = +2
Query: 41 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNE-SFGFTADL 217
EP + I P VG + + + RRG E + + +F+ K LP+ E F +L
Sbjct: 466 EPTVIATIILPSEYVGAVINLCSDRRGQQLEYTFIDAQRVFL-KYQLPLREIVVDFYDEL 524
Query: 218 RSNTGGQA 241
+S T G A
Sbjct: 525 KSITSGYA 532
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 29.1 bits (62), Expect = 1.9
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -2
Query: 326 PVQRCRACSEVRTGLQG 276
PV RCR C+EV G QG
Sbjct: 62 PVHRCRLCAEVHIGKQG 78
>At2g35490.1 68415.m04347 plastid-lipid associated protein PAP,
putative similar to plastid-lipid associated protein
PAP3 [Brassica rapa] GI:14248552; contains Pfam profile
PF04755: PAP_fibrillin
Length = 376
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +2
Query: 254 VFDHWQVLPGDPCEPQSKPYNV 319
V D W PGD EP S+P NV
Sbjct: 71 VTDEWGEKPGDANEPDSQPDNV 92
>At4g23820.1 68417.m03425 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase PG1 [Glycine max]
GI:5669846; contains PF00295: Glycosyl hydrolases family
28
Length = 444
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +1
Query: 115 SWSRFRRVPGGRY-TYVHCEGL 177
S+ R R +PGGRY +++H +GL
Sbjct: 123 SYGRGRELPGGRYMSFIHGDGL 144
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.5 bits (58), Expect = 5.7
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +3
Query: 174 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGRSS 278
P ++ + L P+ VP+ + RP++S + +G +S
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLAS 573
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 27.1 bits (57), Expect = 7.5
Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 3/46 (6%)
Frame = +3
Query: 177 TYLSMSRSVLLPICVPTPADRP---SRSAYSTIGRSSLETRANLRA 305
+Y S SRSV P +P RP SRS YS++ RS +++ R+
Sbjct: 212 SYSSKSRSVS-PARSISPRSRPLSRSRSLYSSVSRSQSRSKSRSRS 256
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,254,161
Number of Sequences: 28952
Number of extensions: 239006
Number of successful extensions: 618
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 599
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 613
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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