Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32201
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 106 2e-24
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 71 8e-14
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 70 2e-13
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 60 3e-10
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 36 0.004
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 35 0.006
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.011
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 32 0.044
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo... 32 0.059
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 29 0.31
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 28 0.72
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 28 0.95
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 28 0.95
SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 1.3
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 26 3.8
SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 5.1
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 25 5.1
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 25 6.7
SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 25 8.9
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 8.9
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.9
SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe... 25 8.9
SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 25 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 106 bits (254), Expect = 2e-24
Identities = 47/144 (32%), Positives = 93/144 (64%)
Frame = +3
Query: 48 EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 227
++++A R+AF +FD + G I ++ ++ ++GQ +ELQ +I+E D + +G I+F
Sbjct: 8 DEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDF 67
Query: 228 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLD 407
F + + +++ D E +E++EAF+++D++GNGYIT L +L +L ++LS ++
Sbjct: 68 TEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 408 GIIAEIDTDGSGTVDFDEFMEMMT 479
+I E DTDG G ++++EF +++
Sbjct: 125 DMIREADTDGDGVINYEEFSRVIS 148
Score = 26.6 bits (56), Expect = 2.2
Identities = 21/106 (19%), Positives = 43/106 (40%), Gaps = 1/106 (0%)
Frame = +3
Query: 60 AMLRKAFQMFDTTKSGYIDVLKISTIL-NTMGQLFDDSELQALIDENDPENSGKINFDGF 236
A L+ D +G ID + T++ M ++ E++ D + +G I +
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEEL 107
Query: 237 CNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAA 374
+H L Q+E+ + R D +G+G I ++++
Sbjct: 108 ----THVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 71.3 bits (167), Expect = 8e-14
Identities = 35/143 (24%), Positives = 74/143 (51%)
Frame = +3
Query: 48 EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 227
E++ + +AF++FD+ K ID ++ + +G + SE+ ++ + D G +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 228 DGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLD 407
+ F + + + E D +E+K AF L+D + G I+ L+ + L++ + + +L+
Sbjct: 93 EDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 408 GIIAEIDTDGSGTVDFDEFMEMM 476
+I E D D G ++ EF+ +M
Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIM 172
Score = 42.3 bits (95), Expect = 4e-05
Identities = 20/74 (27%), Positives = 39/74 (52%)
Frame = +3
Query: 258 LEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDG 437
L E E +Q++ EAF+L+D + + I L+ + AL +++ I+ + D G
Sbjct: 27 LRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTG 86
Query: 438 SGTVDFDEFMEMMT 479
G + ++F+ +MT
Sbjct: 87 KGYLQMEDFVRVMT 100
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 69.7 bits (163), Expect = 2e-13
Identities = 42/146 (28%), Positives = 80/146 (54%)
Frame = +3
Query: 33 MDSDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENS 212
M + EQ M ++AF ++D K G I + ++L ++G D+EL L +E
Sbjct: 1 MPASKEQTDEM-KEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----G 55
Query: 213 GKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLS 392
I+ F + S+ L E ++E +E +AFR++D++ +GYI T+ + + L +KLS
Sbjct: 56 DAIDEKKFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLS 112
Query: 393 NADLDGIIAEIDTDGSGTVDFDEFME 470
+ ++ ++ E D SG+ D+ +F++
Sbjct: 113 DNEVQLMVQEADPTNSGSFDYYDFVQ 138
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 59.7 bits (138), Expect = 3e-10
Identities = 44/154 (28%), Positives = 78/154 (50%), Gaps = 6/154 (3%)
Frame = +3
Query: 39 SDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGK 218
S +++ +RK F D +SG ID + +I + S L +++DE + G
Sbjct: 17 SFSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDE---DGGGD 72
Query: 219 INFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL------D 380
++F F N S F + E ++LK AF++YD + +GYI+ L +L + +
Sbjct: 73 VDFQEFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRE 129
Query: 381 DKLSNADLDGIIAEIDTDGSGTVDFDEFMEMMTG 482
D+L +D I E+D D G + F+EF ++++G
Sbjct: 130 DQLQQI-VDKTIMEVDKDRDGKISFEEFKDIVSG 162
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 35.9 bits (79), Expect = 0.004
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +3
Query: 288 QELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 428
QELKEAF L D++G+G I +K +L +L+ S ++ + I+
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 35.1 bits (77), Expect = 0.006
Identities = 18/60 (30%), Positives = 35/60 (58%)
Frame = +3
Query: 297 KEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 476
K+AF L+DR G G I +++ ++L A + A+ I EI++ VD ++F++++
Sbjct: 9 KQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQVL 64
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 34.3 bits (75), Expect = 0.011
Identities = 21/64 (32%), Positives = 32/64 (50%)
Frame = +3
Query: 282 MQQELKEAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDE 461
M +E +EAF L+D GYI L+ A L + L+ L ++ T+G V +E
Sbjct: 10 MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK--VSREE 67
Query: 462 FMEM 473
F E+
Sbjct: 68 FAEL 71
Score = 32.7 bits (71), Expect = 0.033
Identities = 17/61 (27%), Positives = 30/61 (49%)
Frame = +3
Query: 72 KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIAS 251
+AF +FD T GYID + +G+ +LQ ++D +GK++ + F +
Sbjct: 16 EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREEFAELWI 73
Query: 252 H 254
H
Sbjct: 74 H 74
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 32.3 bits (70), Expect = 0.044
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +3
Query: 264 EEDAEAMQQELKEAFRLYDREGNGYITTSTLKE 362
++D M Q LKE Y+ E N IT+ TLKE
Sbjct: 522 KDDLTEMNQRLKEQIESYENEVNSEITSRTLKE 554
>SPBC11G11.02c |end3||actin cortical patch component End3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 375
Score = 31.9 bits (69), Expect = 0.059
Identities = 22/55 (40%), Positives = 29/55 (52%)
Frame = +3
Query: 300 EAFRLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEF 464
E FR + E NGY++ S +L + KLS+ L+ I D D G DFDEF
Sbjct: 11 EIFRGLNPE-NGYLSGSKAAGVLRS--SKLSSDKLEKIWDLADIDDDGMFDFDEF 62
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 29.5 bits (63), Expect = 0.31
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +3
Query: 120 LKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHF 257
+ +ST+ +T + DDS L+ +ID+ PE GK NI++HF
Sbjct: 12 ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGKPK----ANISTHF 52
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 28.3 bits (60), Expect = 0.72
Identities = 27/112 (24%), Positives = 49/112 (43%), Gaps = 1/112 (0%)
Frame = +3
Query: 45 DEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSEL-QALIDENDPENSGKI 221
D++++ K F F SG+++ + I D S + + + D + +G I
Sbjct: 23 DKKELQQWYKGF--FKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYI 80
Query: 222 NFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEILAAL 377
+F F S E + +L AF+LYD + NG I+ + I+ A+
Sbjct: 81 DFKEFICALSVTSRGE----LNDKLIWAFQLYDLDNNGLISYDEMLRIVDAI 128
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 27.9 bits (59), Expect = 0.95
Identities = 19/81 (23%), Positives = 40/81 (49%), Gaps = 3/81 (3%)
Frame = +3
Query: 114 DVLKISTILNTM-GQLFDDSELQALIDENDP--ENSGKINFDGFCNIASHFLEEEDAEAM 284
+ ++I+ L + G+ D ++ + + +P ENS N D +A+ FL+E + E
Sbjct: 402 ETIRIAVNLQELIGKYLGDKDIFQIENFINPIEENSADTNDDIVNQVAAQFLDEREFETD 461
Query: 285 QQELKEAFRLYDREGNGYITT 347
++E + + L ++ I T
Sbjct: 462 EEEEESQYLLSTKDAINAINT 482
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 27.9 bits (59), Expect = 0.95
Identities = 29/116 (25%), Positives = 44/116 (37%)
Frame = +3
Query: 129 STILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAF 308
ST + + ++ D+ Q+ +D +S D NI + E+ + Q F
Sbjct: 256 STKSSKLDEISADNNAQSAVDNK--YSSPPQTIDPQANIKALITPEDRSNFYQ-----LF 308
Query: 309 RLYDREGNGYITTSTLKEILAALDDKLSNADLDGIIAEIDTDGSGTVDFDEFMEMM 476
D E GYI A L + +L I +DT G +D DEF M
Sbjct: 309 SKIDNENKGYIVGGEAVPFFMA--SHLDSEELARIWDTVDTQDRGYIDKDEFAVAM 362
>SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 418
Score = 27.5 bits (58), Expect = 1.3
Identities = 15/68 (22%), Positives = 29/68 (42%)
Frame = +3
Query: 186 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDREGNGYITTSTLKEI 365
+ E + F F + ++ +D A+ Q + +F +R G G I T ++
Sbjct: 343 VSEESQVEPNTLIFQNFSSPSTSLSAPKDTPAVIQSINTSFLNNERAGRGNIKTVDQNQL 402
Query: 366 LAALDDKL 389
+ + DKL
Sbjct: 403 INRIVDKL 410
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1183
Score = 25.8 bits (54), Expect = 3.8
Identities = 17/68 (25%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = +3
Query: 312 LYDREGNGYITT-STLKEILAALDDKLSNADLDGII-AEIDTDGSGTVDFDEFMEMMTGD 485
LYD+E GY+T + E++ + D+ N D + ++ + SG V E+
Sbjct: 1055 LYDKEKRGYVTLFPSGSEVVQFVPDRTYNIDSEYVVYLSLHESRSGRVYMSPLTEISPEH 1114
Query: 486 *AQRSKDS 509
A+ +K++
Sbjct: 1115 LARLAKNT 1122
>SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 5.1
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +3
Query: 321 REGNGYITTSTLKEILAALDDKLSNADLDGIIAEID 428
RE GY+ T + D L+NA+L I+A+ID
Sbjct: 107 REETGYVGTVMDSTTVMYNDPGLTNANLKIILADID 142
>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 5.1
Identities = 20/100 (20%), Positives = 41/100 (41%), Gaps = 5/100 (5%)
Frame = +3
Query: 186 IDENDPENSGKINFDGFCNIASHFLEEEDAEAMQQ---ELKEAFRLYDREGNGYITTSTL 356
I +N P + IN + ++ EE E KE LY+++ G++ +
Sbjct: 181 IMKNSPMPAEPINKENINQLSRSLFYEEGKEFWDDYDIAAKEILSLYEQDPEGFVNPYSK 240
Query: 357 KEILAALDDKLSNADLDGIIAEI--DTDGSGTVDFDEFME 470
+ A + + + G+ A+ D D + +EF++
Sbjct: 241 RGAAALANSENNKGSEAGVSAKTNPDMDSDSSAIVNEFLK 280
>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 25.0 bits (52), Expect = 6.7
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = +3
Query: 222 NFDGFCNIASHFLEEEDAEAM 284
+ DGF ++ASHF AE++
Sbjct: 269 SIDGFVSVASHFSSASTAESL 289
>SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 24.6 bits (51), Expect = 8.9
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +3
Query: 192 ENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFR 311
E D EN ++ A LE+ED EAM +L+E R
Sbjct: 152 EIDKENLAVLSQKSVLKTAEE-LEKEDREAMSAKLQELIR 190
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 24.6 bits (51), Expect = 8.9
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +3
Query: 189 DENDPENSGKINFDGFCNIASHFLEEEDAEAMQQELKEAFRLYDR 323
D N+PE+ GF +I S +E D E+ + +YDR
Sbjct: 645 DCNNPESFSLFVDAGFISITSQNVETNDKESNSESETLYHLIYDR 689
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1379
Score = 24.6 bits (51), Expect = 8.9
Identities = 9/16 (56%), Positives = 14/16 (87%)
Frame = -3
Query: 472 ISMNSSKSTVPEPSVS 425
+S+ SS +T+P+PSVS
Sbjct: 440 LSLKSSDATIPKPSVS 455
>SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 504
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +3
Query: 93 TTKSGYIDVLKISTILNTMGQLFDDSE 173
TTKS +I++ ST + ++F+DS+
Sbjct: 143 TTKSRFIEISATSTTVADCRKIFEDSQ 169
>SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 577
Score = 24.6 bits (51), Expect = 8.9
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = +1
Query: 139 STQWDSCSMTQNFKHSSMKTIRKTVGRSTSMASVILLPISLKRKTLKPCN 288
S Q D + + +K + I G S ++ + LP SL +KPCN
Sbjct: 356 SYQSDLVAAIKKYKINLRSLIIIDEGVSNTVKDLAFLPQSLTTLIVKPCN 405
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,007,660
Number of Sequences: 5004
Number of extensions: 37738
Number of successful extensions: 164
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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