Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32199
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74510.2 68414.m08632 kelch repeat-containing F-box family pr... 30 1.1
At1g74510.1 68414.m08631 kelch repeat-containing F-box family pr... 30 1.1
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 29 1.9
At5g28210.1 68418.m03416 mRNA capping enzyme family protein simi... 29 2.5
At1g72110.1 68414.m08335 expressed protein 29 2.5
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 3.2
At1g05790.1 68414.m00605 lipase class 3 family protein contains ... 28 3.2
At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to a... 27 5.7
At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to e... 27 5.7
At3g13780.1 68416.m01739 expressed protein 27 5.7
At1g26930.1 68414.m03283 kelch repeat-containing F-box family pr... 27 5.7
At1g09070.1 68414.m01012 C2 domain-containing protein / src2-lik... 27 5.7
At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein 27 7.5
At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containi... 27 7.5
At5g56970.1 68418.m07111 FAD-binding domain-containing protein /... 27 9.9
At3g53080.1 68416.m05850 galactose-binding lectin family protein... 27 9.9
At1g53450.2 68414.m06059 expressed protein 27 9.9
At1g53450.1 68414.m06058 expressed protein 27 9.9
>At1g74510.2 68414.m08632 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 194 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 364
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At1g74510.1 68414.m08631 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 451
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/59 (33%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 194 KVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPP--AVPVVAQG 364
K S G E + FG EI Y Y TN + + VPR L G+ + V+A G
Sbjct: 185 KESLAVGTELLVFGKEIMSHVIYRYSILTNTWTSGMQMNVPRCLFGSASLGEIAVIAGG 243
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 29.1 bits (62), Expect = 1.9
Identities = 21/66 (31%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +2
Query: 278 TNNGIAAQEQGVPRNLGGNPPAVPVVAQGS--FSWTSPEGVPISVNYVADENGYQPTGNA 451
T+ G + G G P P + S +S TSP P S +Y Y PT +
Sbjct: 1555 TSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPS 1614
Query: 452 I-PTSP 466
PTSP
Sbjct: 1615 YSPTSP 1620
Score = 28.7 bits (61), Expect = 2.5
Identities = 22/72 (30%), Positives = 27/72 (37%), Gaps = 1/72 (1%)
Frame = +2
Query: 254 GSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGY 433
G + ++ G + G G P P +S TSP P S Y GY
Sbjct: 1533 GGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSP-----GYSPTSPGYSPTSPTYSPSSPGY 1587
Query: 434 QPTGNAI-PTSP 466
PT A PTSP
Sbjct: 1588 SPTSPAYSPTSP 1599
Score = 28.7 bits (61), Expect = 2.5
Identities = 20/54 (37%), Positives = 25/54 (46%), Gaps = 4/54 (7%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSPPVPEQIAR---ALAYIAKN 514
S+S TSP P S Y Y PT + PTSP Q A+ ++AY N
Sbjct: 1691 SYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSN 1744
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1614 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1648
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1621 SYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSP 1655
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S +Y Y PT A PTSP
Sbjct: 1670 SYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSP 1704
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/35 (45%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S Y GY PT + PTSP
Sbjct: 1684 SYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSP 1718
Score = 27.5 bits (58), Expect = 5.7
Identities = 27/97 (27%), Positives = 39/97 (40%), Gaps = 5/97 (5%)
Frame = +2
Query: 191 PKVSEGYGAETVKFGNEINPDGSYTY--FYETNNGIAAQEQGVPRNLGGNPPAVPVVA-- 358
P S GY + + + +P S T + T+ G + + G P P +
Sbjct: 1539 PSSSPGYSPSSPGY-SPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPT 1597
Query: 359 QGSFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1598 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1634
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1607 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1641
Score = 27.1 bits (57), Expect = 7.5
Identities = 16/35 (45%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S Y Y PT A PTSP
Sbjct: 1628 SYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSP 1662
Score = 27.1 bits (57), Expect = 7.5
Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S +Y Y PT + PTSP
Sbjct: 1663 SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP 1697
Score = 26.6 bits (56), Expect = 9.9
Identities = 15/35 (42%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +2
Query: 365 SFSWTSPEGVPISVNYVADENGYQPTGNAI-PTSP 466
S+S TSP P S +Y Y PT PTSP
Sbjct: 1677 SYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSP 1711
>At5g28210.1 68418.m03416 mRNA capping enzyme family protein similar
to mRNA capping enzyme [Xenopus laevis] GI:7239232;
contains Pfam profiles PF01331: mRNA capping enzyme
catalytic domain, PF03919: mRNA capping enzyme
C-terminal domain
Length = 625
Score = 28.7 bits (61), Expect = 2.5
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -2
Query: 395 EHLQEMSRRMSPVQRREQQGGFHQGYVELPVLGQ 294
E+ +E S+R+ P R+Q+ QG+++ P GQ
Sbjct: 27 ENREEHSKRLRPTSFRDQKVTIPQGWLDCPRFGQ 60
>At1g72110.1 68414.m08335 expressed protein
Length = 479
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +2
Query: 209 YGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNL 325
+G+ T+ F N + PD ++F IAA GVP+ L
Sbjct: 388 FGSTTMIFSNVVGPDEEISFFGHRIAYIAASTFGVPQAL 426
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 28.3 bits (60), Expect = 3.2
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = +3
Query: 318 VTLVETPLLFPSLHRAHSPGHLLKVFPSASITSPTRT 428
++LV TPLLF + A + G LL+ FPS + +SP +
Sbjct: 518 LSLVTTPLLFKLIPSAMNLGVLLRWFPSEN-SSPNES 553
>At1g05790.1 68414.m00605 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 687
Score = 28.3 bits (60), Expect = 3.2
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = -3
Query: 460 SGDSITSGLVSVLVGDVIDADGNTFR 383
SG+S +SG +S L+GD + DG + R
Sbjct: 398 SGESESSGFLSSLIGDGCECDGYSIR 423
>At5g39300.1 68418.m04759 expansin, putative (EXP25) similar to
alpha-expansin 4 precursor GI:16923355 from [Cucumis
sativus]; alpha-expansin gene family, PMID:11641069
Length = 260
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 164 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 57
++GW + W + T G+ TG G S R+ G+
Sbjct: 201 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 236
>At5g39280.1 68418.m04757 expansin, putative (EXP23) similar to
expansin2 GI:4884433 from [Lycopersicon esculentum];
alpha-expansin gene family, PMID:11641069
Length = 259
Score = 27.5 bits (58), Expect = 5.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 164 RSGWAETE*EWSRERTAGMCCTGMGSSCRIHFHTGL 57
++GW + W + T G+ TG G S R+ G+
Sbjct: 200 KTGWITMQKNWGQNWTTGVNLTGQGISFRVTTSDGV 235
>At3g13780.1 68416.m01739 expressed protein
Length = 309
Score = 27.5 bits (58), Expect = 5.7
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 184 SDGGNRGDRDGQKRSRSGHGNER 116
++G + GD DG K+ R G G +R
Sbjct: 177 TNGNSDGDEDGDKKGRKGLGKKR 199
>At1g26930.1 68414.m03283 kelch repeat-containing F-box family
protein contains Pfam:PF01344 Kelch motif, Pfam:PF00646
F-box domain
Length = 421
Score = 27.5 bits (58), Expect = 5.7
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = +2
Query: 182 RVVPKVSEGYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGN 334
R K S G + + FG E++ Y Y TN+ A+ +PR L G+
Sbjct: 157 RYADKESLAVGTDLLVFGWEVSSYVIYRYSLLTNSWSTAKSMNMPRCLFGS 207
>At1g09070.1 68414.m01012 C2 domain-containing protein / src2-like
protein, putative similar to cold-regulated gene SRC2
[Glycine max] GI:2055230; contains Pfam profile PF00168:
C2 domain; identical to cDNA src2-like protein
GI:3426059
Length = 324
Score = 27.5 bits (58), Expect = 5.7
Identities = 17/55 (30%), Positives = 21/55 (38%), Gaps = 6/55 (10%)
Frame = +2
Query: 299 QEQGVPRNLGGNPPAVPVVAQGSFSWTSPE------GVPISVNYVADENGYQPTG 445
Q+ G P GG PP QG + P+ G P Y + GY P G
Sbjct: 204 QQAGYPAGTGGYPPPGAYPQQGGYPGYPPQQQGGYPGYPPQGPYGYPQQGYPPQG 258
>At5g49360.1 68418.m06108 glycosyl hydrolase family 3 protein
Length = 774
Score = 27.1 bits (57), Expect = 7.5
Identities = 17/62 (27%), Positives = 28/62 (45%)
Frame = +2
Query: 317 RNLGGNPPAVPVVAQGSFSWTSPEGVPISVNYVADENGYQPTGNAIPTSPPVPEQIARAL 496
RNL N AVP + G + W S +++ ++D G A P + P+ I A
Sbjct: 74 RNLVNNAAAVPRLGIGGYEWWSE-----ALHGISDVGPGAKFGGAFPGATSFPQVITTAA 128
Query: 497 AY 502
++
Sbjct: 129 SF 130
>At4g22760.1 68417.m03286 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 889
Score = 27.1 bits (57), Expect = 7.5
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +1
Query: 52 RPSPV*K*ILQEEPIPVQHIPAVRSRDHSYSVSAHPDLLYCPRQSS 189
RPSP+ ++EE P+ ++P +RS + S S LY P SS
Sbjct: 67 RPSPMRGLPMEEETKPIVYLPRIRSEVFASSPSLLN--LYSPSSSS 110
>At5g56970.1 68418.m07111 FAD-binding domain-containing protein /
cytokinin oxidase family protein similar to cytokinin
oxidase from Zea mays [gi:3882018] [gi:3341978]
Length = 523
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 375 GHLLKVFPSASITSPTRTDTSPLVMLS 455
GH+ K+FPSA + + D + L+ LS
Sbjct: 63 GHVTKIFPSAVLIPSSVEDITDLIKLS 89
>At3g53080.1 68416.m05850 galactose-binding lectin family protein
contains Pfam domain PF02140: Galactose binding lectin
domain
Length = 155
Score = 26.6 bits (56), Expect = 9.9
Identities = 17/65 (26%), Positives = 27/65 (41%)
Frame = +2
Query: 206 GYGAETVKFGNEINPDGSYTYFYETNNGIAAQEQGVPRNLGGNPPAVPVVAQGSFSWTSP 385
GY + F + NP G+ +F N G A + V +N G +V F +
Sbjct: 81 GYVITKINFADYGNPTGTCGHFRRGNCGARATMRIVKKNCLGKEKCHLLVTDEMFGPSKC 140
Query: 386 EGVPI 400
+G P+
Sbjct: 141 KGAPM 145
>At1g53450.2 68414.m06059 expressed protein
Length = 453
Score = 26.6 bits (56), Expect = 9.9
Identities = 19/48 (39%), Positives = 24/48 (50%)
Frame = +3
Query: 357 HRAHSPGHLLKVFPSASITSPTRTDTSPLVMLSPLPHQCLSRSLVLLP 500
H + G L +V S S SP R D+S L +L P L S +LLP
Sbjct: 185 HSLAARGDLWRVEASTS-NSPVRDDSSSLFLLQLGPLLFLRDSTLLLP 231
>At1g53450.1 68414.m06058 expressed protein
Length = 453
Score = 26.6 bits (56), Expect = 9.9
Identities = 19/48 (39%), Positives = 24/48 (50%)
Frame = +3
Query: 357 HRAHSPGHLLKVFPSASITSPTRTDTSPLVMLSPLPHQCLSRSLVLLP 500
H + G L +V S S SP R D+S L +L P L S +LLP
Sbjct: 185 HSLAARGDLWRVEASTS-NSPVRDDSSSLFLLQLGPLLFLRDSTLLLP 231
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,756,306
Number of Sequences: 28952
Number of extensions: 263501
Number of successful extensions: 877
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 865
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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