BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV32161 (385 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.11 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.33 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 27 0.76 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 0.76 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 26 2.3 SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 4.1 SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 24 7.1 SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 24 9.4 SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 24 9.4 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 30.3 bits (65), Expect = 0.11 Identities = 22/79 (27%), Positives = 33/79 (41%), Gaps = 3/79 (3%) Frame = -3 Query: 245 VRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLS--SAESPKLP 72 V P A P+P V +P P + +P + P P + S+E+P + Sbjct: 1191 VPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVS 1250 Query: 71 NPESWCPNPES-WCPNPES 18 P S P+P S P+P S Sbjct: 1251 TPRSSVPSPHSNASPSPTS 1269 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.7 bits (61), Expect = 0.33 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = -3 Query: 236 PPMAVPIPPNEVAMPSRPFSILSSSIPWSA 147 PPM+VP PP+ MP+ P S +P S+ Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 27.5 bits (58), Expect = 0.76 Identities = 17/63 (26%), Positives = 31/63 (49%) Frame = -3 Query: 233 PMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPESWC 54 P + P PP + + S+P S SS++P K F+ + S ++++ P+ + ES Sbjct: 193 PYSSPFPPPRIVLGSKPVS--SSTLPSKPKSFQVFSDASSSRDSQNASDLPQAKSLESEA 250 Query: 53 PNP 45 P Sbjct: 251 NTP 253 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 27.5 bits (58), Expect = 0.76 Identities = 24/77 (31%), Positives = 35/77 (45%), Gaps = 2/77 (2%) Frame = -3 Query: 260 FAIFEVRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPK-LSSAES 84 F F R P P N ++PS P SSS P + + + + + +P L S++ Sbjct: 98 FKNFPFRPPTRLTSTPSNSSSLPSIP---SSSSTPSISSIPHTTSSVSNDIPSVLGSSDH 154 Query: 83 P-KLPNPESWCPNPESW 36 P L NPE P P S+ Sbjct: 155 PIDLDNPEHLTP-PSSF 170 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 25.8 bits (54), Expect = 2.3 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 4/39 (10%) Frame = -3 Query: 131 FTCP--ALSLPKLSSA--ESPKLPNPESWCPNPESWCPN 27 +T P A+ +P L A +S P PE++ NP+ W PN Sbjct: 403 YTVPKDAMVIPTLYGALHDSKVYPEPETF--NPDRWAPN 439 >SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 700 Score = 25.0 bits (52), Expect = 4.1 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = -3 Query: 242 RAPPMAVPIPPNEVAMPSRPFS 177 R P M +P PP++ +PS P S Sbjct: 24 RQPSMNLPTPPHDDGLPSIPRS 45 >SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1191 Score = 24.2 bits (50), Expect = 7.1 Identities = 20/58 (34%), Positives = 26/58 (44%) Frame = -3 Query: 239 APPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNP 66 AP + + P + +P RP LS E+S PAL+ LSS SP P P Sbjct: 347 APASEMSLIPEKPRLPPRPSHTLS---------ELS--SPALTSENLSSKPSPLFPPP 393 >SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 23.8 bits (49), Expect = 9.4 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -3 Query: 191 SRPFSILSSSIPWSAKLEISFT 126 ++P SI +S PWS + ++SF+ Sbjct: 301 NQPISIYIASGPWSLRDDLSFS 322 >SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1275 Score = 23.8 bits (49), Expect = 9.4 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = -3 Query: 218 IPPNEVAMPSRPFSILSSSIPWSAK 144 +P + + + PFS+LSS P S++ Sbjct: 835 VPGSTSPLNASPFSVLSSDFPGSSR 859 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,375,229 Number of Sequences: 5004 Number of extensions: 27763 Number of successful extensions: 93 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 88 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 92 length of database: 2,362,478 effective HSP length: 65 effective length of database: 2,037,218 effective search space used: 126307516 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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