BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32161
(385 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.11
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.33
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 27 0.76
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 0.76
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 26 2.3
SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 4.1
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 24 7.1
SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 24 9.4
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 24 9.4
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 30.3 bits (65), Expect = 0.11
Identities = 22/79 (27%), Positives = 33/79 (41%), Gaps = 3/79 (3%)
Frame = -3
Query: 245 VRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLS--SAESPKLP 72
V P A P+P V +P P + +P + P P + S+E+P +
Sbjct: 1191 VPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVS 1250
Query: 71 NPESWCPNPES-WCPNPES 18
P S P+P S P+P S
Sbjct: 1251 TPRSSVPSPHSNASPSPTS 1269
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.7 bits (61), Expect = 0.33
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -3
Query: 236 PPMAVPIPPNEVAMPSRPFSILSSSIPWSA 147
PPM+VP PP+ MP+ P S +P S+
Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 27.5 bits (58), Expect = 0.76
Identities = 17/63 (26%), Positives = 31/63 (49%)
Frame = -3
Query: 233 PMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPESWC 54
P + P PP + + S+P S SS++P K F+ + S ++++ P+ + ES
Sbjct: 193 PYSSPFPPPRIVLGSKPVS--SSTLPSKPKSFQVFSDASSSRDSQNASDLPQAKSLESEA 250
Query: 53 PNP 45
P
Sbjct: 251 NTP 253
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.5 bits (58), Expect = 0.76
Identities = 24/77 (31%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = -3
Query: 260 FAIFEVRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPK-LSSAES 84
F F R P P N ++PS P SSS P + + + + + +P L S++
Sbjct: 98 FKNFPFRPPTRLTSTPSNSSSLPSIP---SSSSTPSISSIPHTTSSVSNDIPSVLGSSDH 154
Query: 83 P-KLPNPESWCPNPESW 36
P L NPE P P S+
Sbjct: 155 PIDLDNPEHLTP-PSSF 170
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 25.8 bits (54), Expect = 2.3
Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 4/39 (10%)
Frame = -3
Query: 131 FTCP--ALSLPKLSSA--ESPKLPNPESWCPNPESWCPN 27
+T P A+ +P L A +S P PE++ NP+ W PN
Sbjct: 403 YTVPKDAMVIPTLYGALHDSKVYPEPETF--NPDRWAPN 439
>SPBC1A4.05 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 700
Score = 25.0 bits (52), Expect = 4.1
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -3
Query: 242 RAPPMAVPIPPNEVAMPSRPFS 177
R P M +P PP++ +PS P S
Sbjct: 24 RQPSMNLPTPPHDDGLPSIPRS 45
>SPAC9G1.10c |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1191
Score = 24.2 bits (50), Expect = 7.1
Identities = 20/58 (34%), Positives = 26/58 (44%)
Frame = -3
Query: 239 APPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNP 66
AP + + P + +P RP LS E+S PAL+ LSS SP P P
Sbjct: 347 APASEMSLIPEKPRLPPRPSHTLS---------ELS--SPALTSENLSSKPSPLFPPP 393
>SPCC553.09c |spb70|pol12|DNA polymerase alpha
B-subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 23.8 bits (49), Expect = 9.4
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -3
Query: 191 SRPFSILSSSIPWSAKLEISFT 126
++P SI +S PWS + ++SF+
Sbjct: 301 NQPISIYIASGPWSLRDDLSFS 322
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 23.8 bits (49), Expect = 9.4
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = -3
Query: 218 IPPNEVAMPSRPFSILSSSIPWSAK 144
+P + + + PFS+LSS P S++
Sbjct: 835 VPGSTSPLNASPFSVLSSDFPGSSR 859
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,375,229
Number of Sequences: 5004
Number of extensions: 27763
Number of successful extensions: 93
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 126307516
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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