BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32155
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1778.02 |rap1||telomere binding protein Rap1|Schizosaccharom... 29 0.55
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 27 2.2
SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 26 2.9
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 25 5.1
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 25 6.7
SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyce... 25 8.9
>SPBC1778.02 |rap1||telomere binding protein
Rap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 693
Score = 28.7 bits (61), Expect = 0.55
Identities = 23/96 (23%), Positives = 43/96 (44%), Gaps = 7/96 (7%)
Frame = +2
Query: 218 NGAIQREAKRLIVKNKR-------FERSQVLAFVVEWIDENKELEWESTFSTSRQHESFR 376
+ ++ + + +V NKR F + + ++ V+++DE+ L + Q R
Sbjct: 218 SASVSKSENKALVNNKRYSDSYFYFSKMRRISIDVDYVDEDLNL----INAYLSQFGKKR 273
Query: 377 HVTELFRHIKRYFSFVEHLHNFSHWHGVFFKYDKFI 484
+ EL + R FS + H FS W +F + FI
Sbjct: 274 SLNELCALLSRRFS---NRHTFSEWRALFMHFFPFI 306
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 26.6 bits (56), Expect = 2.2
Identities = 24/74 (32%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
Frame = +3
Query: 144 GNRSPSW*MKISLKYMLG-TLNNASTTGRSRGKPRGLLSRTKDSKGAKSLPLLSNG*TKT 320
GN PS+ K S Y G NN+ G PR SR + +SNG +
Sbjct: 308 GNSHPSY-NKYS-HYQHGFNYNNSGNNRNESGHPRFRNSRRNYNNQGAYPTYMSNGRSAN 365
Query: 321 KSWNGNPPSVPLGS 362
+S NP +V GS
Sbjct: 366 QSPRNNPQNVNNGS 379
>SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 598
Score = 26.2 bits (55), Expect = 2.9
Identities = 20/70 (28%), Positives = 27/70 (38%)
Frame = +3
Query: 207 NASTTGRSRGKPRGLLSRTKDSKGAKSLPLLSNG*TKTKSWNGNPPSVPLGSMSLLGM*Q 386
+A TTG S GKP G + T KG + G + K + P S
Sbjct: 264 SAPTTGFSFGKPAGQAASTATDKGTTTTSSAGTGFSFGKPATTEDTNKPTAPNSAFTKPA 323
Query: 387 NSSDISKGTF 416
S+ +K TF
Sbjct: 324 TSTGDNKPTF 333
>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 703
Score = 25.4 bits (53), Expect = 5.1
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +2
Query: 101 NIRLCVIRRKIEFVREPFTLVVNENILEI 187
N+ C++ RKIE E T V N++ +E+
Sbjct: 488 NVMNCLLYRKIENFEEVLTWVYNKSHIEL 516
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 25.0 bits (52), Expect = 6.7
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 1/44 (2%)
Frame = +2
Query: 32 FLHFLWILRMQNSSVY*NLI-FWNNIRLCVIRRKIEFVREPFTL 160
FL W+ Q + +LI WN I CV+ IE VR T+
Sbjct: 91 FLKSSWLPLKQKRVLTSSLIKLWNEIHDCVLFDAIEHVRSIATI 134
>SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 906
Score = 24.6 bits (51), Expect = 8.9
Identities = 8/20 (40%), Positives = 14/20 (70%)
Frame = -3
Query: 439 IMKMLDEGKVPFDMSEEFCY 380
I K++D G++ FD + + CY
Sbjct: 472 IRKLIDIGEIDFDQAFKICY 491
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,219,744
Number of Sequences: 5004
Number of extensions: 48427
Number of successful extensions: 106
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 103
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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