Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= epV32136
         (476 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    97   9e-22
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    59   3e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    56   2e-09
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    52   6e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    49   3e-07
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    35   0.007
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    34   0.010
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    33   0.022
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    31   0.091
SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc...    26   2.6  
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma...    25   4.5  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 97.5 bits (232), Expect = 9e-22
 Identities = 48/120 (40%), Positives = 78/120 (65%)
 Frame = +3

Query: 21  LPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRL 200
           L  EQIA  R+AF  FD ++ G+I  + +  ++R +GQ      L+++I EVDAD +G +
Sbjct: 6   LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65

Query: 201 EFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREILRELDEQLTDDE 380
           +F EF+T+ A+ + + D E   +E+REAF+++DK+GNGYI    L  +L  L E+L+ +E
Sbjct: 66  DFTEFLTMMARKMKDTDNE---EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122



 Score = 29.5 bits (63), Expect = 0.28
 Identities = 10/17 (58%), Positives = 14/17 (82%)
 Frame = +1

Query: 391 LIQEIDTDGSGTVDFDE 441
           +I E+D DG+GT+DF E
Sbjct: 53  MINEVDADGNGTIDFTE 69



 Score = 25.8 bits (54), Expect = 3.4
 Identities = 8/17 (47%), Positives = 14/17 (82%)
 Frame = +1

Query: 391 LIQEIDTDGSGTVDFDE 441
           +I+E DTDG G ++++E
Sbjct: 126 MIREADTDGDGVINYEE 142


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 59.3 bits (137), Expect = 3e-10
 Identities = 34/121 (28%), Positives = 62/121 (51%)
 Frame = +3

Query: 18  ELPPEQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGR 197
           E+  EQ   + +AF  FD ++  +I    +   +R +G    K  + +++ + D    G 
Sbjct: 30  EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89

Query: 198 LEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREILRELDEQLTDD 377
           L+  +FV +  + IVE D     +E++ AF L+D +  G I   +LR + +EL+E + D 
Sbjct: 90  LQMEDFVRVMTEKIVERDP---LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQ 146

Query: 378 E 380
           E
Sbjct: 147 E 147



 Score = 38.3 bits (85), Expect = 6e-04
 Identities = 16/60 (26%), Positives = 34/60 (56%)
 Frame = +3

Query: 45  LRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTL 224
           +++AF+ FD + +G I    +  + + + +  + + LE +IEE D D+ G +   EF+ +
Sbjct: 112 IKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAI 171


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 56.4 bits (130), Expect = 2e-09
 Identities = 33/117 (28%), Positives = 65/117 (55%)
 Frame = +3

Query: 30  EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 209
           EQ   +++AF  +D ++ G IP   V  +LR +G       L +L  E+       ++  
Sbjct: 6   EQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAIDEK 61

Query: 210 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREILRELDEQLTDDE 380
           +F++  +  + E ++E   +E  +AFR++DK+ +GYI T+   + ++ L E+L+D+E
Sbjct: 62  KFMSFVSNKLRETESE---EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNE 115


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 51.6 bits (118), Expect = 6e-08
 Identities = 34/107 (31%), Positives = 54/107 (50%)
 Frame = +3

Query: 30  EQIAVLRKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFP 209
           E+I  +RK F   D N+SGSI  +    I  +   P    +   L   VD D  G ++F 
Sbjct: 21  EEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNP----LASRLFSVVDEDGGGDVDFQ 76

Query: 210 EFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREILR 350
           EF+   + F V  + E   ++L+ AF++YD + +GYI    L  +L+
Sbjct: 77  EFINSLSVFSVHGNKE---EKLKFAFKIYDIDRDGYISNGELYLVLK 120


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 49.2 bits (112), Expect = 3e-07
 Identities = 31/111 (27%), Positives = 58/111 (52%)
 Frame = +3

Query: 48  RKAFDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEFVTLA 227
           ++AF  FD + +G IP   + D+LR  GQ  N  + E  I E+++     ++  +F+ + 
Sbjct: 9   KQAFSLFDRHGTGRIPKTSIGDLLRACGQ--NPTLAE--ITEIESTLPAEVDMEQFLQVL 64

Query: 228 AKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREILRELDEQLTDDE 380
            +     D     +E  + F+++DK+  G I    LR +L  L E+L+++E
Sbjct: 65  NR-PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEE 114


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 34.7 bits (76), Expect = 0.007
 Identities = 25/68 (36%), Positives = 37/68 (54%)
 Frame = +3

Query: 156 EELIEEVDADKSGRLEFPEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSL 335
           E +    DADK+G ++F EF+   +   V    E +  +L  AF+LYD + NG I   S 
Sbjct: 66  EYVFNVFDADKNGYIDFKEFICALS---VTSRGE-LNDKLIWAFQLYDLDNNGLI---SY 118

Query: 336 REILRELD 359
            E+LR +D
Sbjct: 119 DEMLRIVD 126


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 34.3 bits (75), Expect = 0.010
 Identities = 14/37 (37%), Positives = 25/37 (67%)
 Frame = +3

Query: 267 KELREAFRLYDKEGNGYIPTSSLREILRELDEQLTDD 377
           +EL+EAF L DK+G+G I    ++ +L  L++  ++D
Sbjct: 48  QELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASED 84


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 33.1 bits (72), Expect = 0.022
 Identities = 16/40 (40%), Positives = 21/40 (52%)
 Frame = +3

Query: 261 MQKELREAFRLYDKEGNGYIPTSSLREILRELDEQLTDDE 380
           M +E  EAF L+D    GYI    LR    +L E LT ++
Sbjct: 10  MDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQ 49


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 31.1 bits (67), Expect = 0.091
 Identities = 15/53 (28%), Positives = 28/53 (52%)
 Frame = +3

Query: 57  FDGFDHNRSGSIPCDFVADILRMMGQPFNKKILEELIEEVDADKSGRLEFPEF 215
           ++  +  +S  +    V  + +M+    + + LEE  ++ DAD SG+L F EF
Sbjct: 303 WEKLEKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355


>SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 26.2 bits (55), Expect = 2.6
 Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 6/63 (9%)
 Frame = +3

Query: 207 PEFVTLAAKFIVEEDAEAMQKELREAFRLYDKEGNGYIPTSSLREILRELD------EQL 368
           PEF   AA+ +VEED E +     EA  + D+EG         R+  + L+      E+L
Sbjct: 38  PEFSDEAAEALVEEDIEDLD---GEALDVEDEEGEDLFGEGMERDYQQNLELDRYDIEEL 94

Query: 369 TDD 377
            DD
Sbjct: 95  DDD 97


>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 614

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 11/22 (50%), Positives = 15/22 (68%)
 Frame = +3

Query: 153 LEELIEEVDADKSGRLEFPEFV 218
           + E I EV+ D SGR+E  +FV
Sbjct: 56  VREAIREVNVDSSGRVEPEDFV 77


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,614,646
Number of Sequences: 5004
Number of extensions: 29161
Number of successful extensions: 136
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)