BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= epV32132 (516 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g33620.1 68417.m04775 Ulp1 protease family protein low simila... 54 6e-08 At1g09730.1 68414.m01092 Ulp1 protease family protein low simila... 52 3e-07 At1g10570.2 68414.m01191 Ulp1 protease family protein contains P... 41 4e-04 At1g10570.1 68414.m01190 Ulp1 protease family protein contains P... 41 4e-04 At3g48480.1 68416.m05292 hypothetical protein 34 0.065 At4g15880.1 68417.m02413 Ulp1 protease family protein contains P... 32 0.26 At1g29110.1 68414.m03563 cysteine proteinase, putative contains ... 31 0.35 At5g60190.1 68418.m07545 Ulp1 protease family protein low simila... 31 0.61 At1g60220.1 68414.m06782 Ulp1 protease family protein contains P... 30 0.80 At2g27980.1 68415.m03391 expressed protein 30 1.1 At4g00690.1 68417.m00094 Ulp1 protease family protein similar to... 29 1.4 At2g12900.1 68415.m01408 hypothetical protein similar to transcr... 29 1.9 At1g09010.1 68414.m01005 glycoside hydrolase family 2 protein lo... 29 1.9 At4g18390.1 68417.m02729 TCP family transcription factor, putati... 27 5.7 At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 27 7.5 At3g06910.1 68416.m00820 Ulp1 protease family protein similar to... 27 7.5 At2g42220.1 68415.m05225 rhodanese-like domain-containing protei... 27 7.5 At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 27 7.5 At1g45090.1 68414.m05169 Ulp1 protease family protein similar to... 27 7.5 At4g14970.1 68417.m02301 hypothetical protein 27 9.9 At4g09280.1 68417.m01536 Ulp1 protease family protein contains P... 27 9.9 At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138... 27 9.9 At2g33740.2 68415.m04137 copper-binding protein (CUTA) identical... 27 9.9 At2g33740.1 68415.m04136 copper-binding protein (CUTA) identical... 27 9.9 >At4g33620.1 68417.m04775 Ulp1 protease family protein low similarity to SP|Q9BQF6 SUMO-1-specific protease 2 (EC 3.4.22.-) (Sentrin-specific protease SENP7) {Homo sapiens}; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 783 Score = 54.0 bits (124), Expect = 6e-08 Identities = 37/127 (29%), Positives = 59/127 (46%), Gaps = 8/127 (6%) Frame = +2 Query: 83 PIKQPCILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNK--DNIKGSCLKIPQQ 256 P + PCIL DS+ G+ + ++ YL E+ + + N++ L++PQQ Sbjct: 429 PQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRAPNMQSISLELPQQ 488 Query: 257 NNFTDCGLYLLQYVEQFFKDPITDYTLPI-----KQLT-NWFDEIVVTRKREEISNLLKS 418 N DCGL+LL Y++ F + + LT NWF + KR I LL + Sbjct: 489 ENSFDCGLFLLHYLDLFVAQAPAKFNPSLISRSANFLTRNWFPAKEASLKRRNILELLYN 548 Query: 419 LMNKYNP 439 L ++P Sbjct: 549 LHKGHDP 555 >At1g09730.1 68414.m01092 Ulp1 protease family protein low similarity to SP|Q9GZR1 SUMO-1-specific protease 1 (EC 3.4.22.-) (Sentrin-specific protease SENP6) (Protease FKSG6) {Homo sapiens}; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 984 Score = 51.6 bits (118), Expect = 3e-07 Identities = 39/121 (32%), Positives = 56/121 (46%), Gaps = 9/121 (7%) Frame = +2 Query: 89 KQPCILIFDSLAGASRSRVVATLRDYLTCEY---HVKVSPLKIFNKDNIKGSCLKIPQQN 259 K PCIL DS+ G S + + ++ YL E+ H + S N++ L++PQQ Sbjct: 567 KVPCILHMDSIKG-SHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQQE 625 Query: 260 NFTDCGLYLLQYVEQFFKD------PITDYTLPIKQLTNWFDEIVVTRKREEISNLLKSL 421 N DCGL+LL Y+E F + P Y NWF + KR I L+ L Sbjct: 626 NSFDCGLFLLHYLELFLAEAPLNFSPFKIYNASNFLYLNWFPPAEASLKRTLIQKLIFEL 685 Query: 422 M 424 + Sbjct: 686 L 686 >At1g10570.2 68414.m01191 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to EST gb|N96456 Length = 570 Score = 41.1 bits (92), Expect = 4e-04 Identities = 23/68 (33%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = +2 Query: 239 LKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTL-PIKQL-TNWFDEIVVTRKREEISNLL 412 +++PQQ N DCGL+LL ++ +F ++ TL +K + WF + R +I N+L Sbjct: 500 VQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASALRIKIWNIL 559 Query: 413 KSLMNKYN 436 L K N Sbjct: 560 VDLFRKGN 567 >At1g10570.1 68414.m01190 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to EST gb|N96456 Length = 571 Score = 41.1 bits (92), Expect = 4e-04 Identities = 23/68 (33%), Positives = 37/68 (54%), Gaps = 2/68 (2%) Frame = +2 Query: 239 LKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTL-PIKQL-TNWFDEIVVTRKREEISNLL 412 +++PQQ N DCGL+LL ++ +F ++ TL +K + WF + R +I N+L Sbjct: 501 VQVPQQKNDFDCGLFLLFFIRRFIEEAPQRLTLQDLKMIHKKWFKPEEASALRIKIWNIL 560 Query: 413 KSLMNKYN 436 L K N Sbjct: 561 VDLFRKGN 568 >At3g48480.1 68416.m05292 hypothetical protein Length = 169 Score = 33.9 bits (74), Expect = 0.065 Identities = 15/58 (25%), Positives = 31/58 (53%), Gaps = 3/58 (5%) Frame = +2 Query: 245 IPQQNNFTDCGLYLLQYVEQFFKDPITDYT---LPIKQLTNWFDEIVVTRKREEISNL 409 +PQQ N +CG ++L Y+ +F +D ++ +P +WF + + +E+ +L Sbjct: 108 VPQQTNDVECGSFVLYYIHRFIEDAPENFNVEDMPYFLKEDWFSHKDLEKFCDELHSL 165 >At4g15880.1 68417.m02413 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; low similarity to sentrin/SUMO-specific protease [Homo sapiens] GI:6906859; identical to cDNA hypothetical protein, partial (1189 bp) GI:2326349 Length = 489 Score = 31.9 bits (69), Expect = 0.26 Identities = 22/69 (31%), Positives = 34/69 (49%) Frame = +2 Query: 101 ILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNKDNIKGSCLKIPQQNNFTDCGL 280 +L DSL G ++ L Y+ E + K S KI +PQQ N DCG+ Sbjct: 393 LLYLDSLNGVD-PMILNALAKYMGDEANEK-SGKKIDANSWDMEFVEDLPQQKNGYDCGM 450 Query: 281 YLLQYVEQF 307 ++L+Y++ F Sbjct: 451 FMLKYIDFF 459 Score = 30.3 bits (65), Expect = 0.80 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = -3 Query: 211 EYFERRYFNMVFTSKIVSQSGYN 143 +Y + YFN F K+VS SGYN Sbjct: 326 KYLKCHYFNTFFYKKLVSDSGYN 348 >At1g29110.1 68414.m03563 cysteine proteinase, putative contains similarity to cysteine protease SPCP1 GI:13491750 from [Ipomoea batatas] Length = 334 Score = 31.5 bits (68), Expect = 0.35 Identities = 27/116 (23%), Positives = 52/116 (44%), Gaps = 4/116 (3%) Frame = +2 Query: 131 SRSRVVATLRDYLTCEYHVK--VSPLKIFNKDNIKGSCLKIPQQNNFTDCGLYLLQYVEQ 304 S++R TL + +YH + +++ ++ K LK+ ++N L+++E Sbjct: 21 SQARPHVTLNEQSIVDYHQQWMTQFSRVYKDESEKEMRLKVFKKN---------LKFIEN 71 Query: 305 FFKDPITDYTLPIKQLTNW-FDEIVVTRKREEIS-NLLKSLMNKYNPDSHLTLPDI 466 F YTL + + T+W +E + T ++ L L NK P + + DI Sbjct: 72 FNNMGNQSYTLGVNEFTDWKTEEFLATHTGLRVNVTSLSELFNKTKPSRNWNMSDI 127 >At5g60190.1 68418.m07545 Ulp1 protease family protein low similarity to sentrin/SUMO-specific protease [Homo sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 226 Score = 30.7 bits (66), Expect = 0.61 Identities = 27/86 (31%), Positives = 38/86 (44%), Gaps = 6/86 (6%) Frame = +2 Query: 191 VSPLKIFNKDNIKGSCLKIPQQNNFTDCGLYLLQYVEQFFKDPITDYTLPIKQLTNWFDE 370 VSP + N D C PQQ N DCG++LL + + +L WF Sbjct: 140 VSPF-VSNGDASYKECTDTPQQKNGYDCGVFLLATARVICEWFSSGGMKNRDEL--WFAN 196 Query: 371 I------VVTRKREEISNLLKSLMNK 430 + +V REEI L+K LM++ Sbjct: 197 VKETVPDLVNHLREEILALIKKLMSE 222 >At1g60220.1 68414.m06782 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 604 Score = 30.3 bits (65), Expect = 0.80 Identities = 10/31 (32%), Positives = 22/31 (70%) Frame = +2 Query: 224 IKGSCLKIPQQNNFTDCGLYLLQYVEQFFKD 316 I + +++PQQ N DCG ++L ++++F ++ Sbjct: 529 ISEAVVQVPQQKNDFDCGPFVLFFIKRFIEE 559 >At2g27980.1 68415.m03391 expressed protein Length = 1037 Score = 29.9 bits (64), Expect = 1.1 Identities = 18/53 (33%), Positives = 29/53 (54%), Gaps = 6/53 (11%) Frame = +2 Query: 374 VVTRKREEISN-LLKSLMNKYNPDS-----HLTLPDITFPTLNGKLIESEDNE 514 ++ R E++SN +L L K P+ + T PDI + L+GKL S+D + Sbjct: 817 LIVRGEEKLSNNILNFLRKKEQPNEENCPDYKTTPDIRWRVLSGKLTSSDDTK 869 >At4g00690.1 68417.m00094 Ulp1 protease family protein similar to SUMO-1/Smt3-specific isopeptidase 2 [Mus musculus] GI:16118473, sentrin/SUMO-specific protease [Homo sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 242 Score = 29.5 bits (63), Expect = 1.4 Identities = 12/23 (52%), Positives = 16/23 (69%) Frame = -3 Query: 211 EYFERRYFNMVFTSKIVSQSGYN 143 +YF+ +FN F K+VS SGYN Sbjct: 71 KYFKCHFFNTFFYVKLVSGSGYN 93 Score = 29.5 bits (63), Expect = 1.4 Identities = 9/18 (50%), Positives = 15/18 (83%) Frame = +2 Query: 248 PQQNNFTDCGLYLLQYVE 301 PQQ N DCG+++L+Y++ Sbjct: 186 PQQQNGYDCGMFMLKYID 203 >At2g12900.1 68415.m01408 hypothetical protein similar to transcription factor(bZIP family) VSF-1 GI:3425907 from [Lycopersicon esculentum] Length = 264 Score = 29.1 bits (62), Expect = 1.9 Identities = 14/29 (48%), Positives = 18/29 (62%) Frame = +2 Query: 149 ATLRDYLTCEYHVKVSPLKIFNKDNIKGS 235 A LRD LT + HV++ LK+ N KGS Sbjct: 190 AQLRDALTEKLHVEIERLKVITISNEKGS 218 >At1g09010.1 68414.m01005 glycoside hydrolase family 2 protein low similarity to mannosidase [gi:5359712] from Cellulomonas fimi Length = 944 Score = 29.1 bits (62), Expect = 1.9 Identities = 14/26 (53%), Positives = 16/26 (61%) Frame = +2 Query: 254 QNNFTDCGLYLLQYVEQFFKDPITDY 331 + NFTD G Y +QY E FFKD Y Sbjct: 520 KGNFTD-GPYEIQYPEDFFKDTYYKY 544 >At4g18390.1 68417.m02729 TCP family transcription factor, putative similar to TFPD (GI:6681577) [Arabidopsis thaliana]; teosinte branched1 protein - Zea mays, PIR2:T04347 Length = 365 Score = 27.5 bits (58), Expect = 5.7 Identities = 11/26 (42%), Positives = 14/26 (53%) Frame = -2 Query: 80 HFYYNTVEVPFCSPADFYHVHHHQNH 3 HF +T PF S +D +H H H H Sbjct: 222 HFPISTNSHPFSSISDHHHHHPHHQH 247 >At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam domain PF04931: DNA polymerase V Length = 1306 Score = 27.1 bits (57), Expect = 7.5 Identities = 15/74 (20%), Positives = 33/74 (44%) Frame = +2 Query: 284 LLQYVEQFFKDPITDYTLPIKQLTNWFDEIVVTRKREEISNLLKSLMNKYNPDSHLTLPD 463 LL +V++ +P+ + + +L WF++ + + LK L K + D + Sbjct: 304 LLDFVDKLPTEPVVTHVMEAPELYKWFEQATEVGNPDALLLALK-LHEKVSVDHPIFSKL 362 Query: 464 ITFPTLNGKLIESE 505 + P +GK ++ Sbjct: 363 LPVPFSSGKFFSAD 376 >At3g06910.1 68416.m00820 Ulp1 protease family protein similar to sentrin/SUMO-specific protease [Homo sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 502 Score = 27.1 bits (57), Expect = 7.5 Identities = 7/19 (36%), Positives = 15/19 (78%) Frame = +2 Query: 245 IPQQNNFTDCGLYLLQYVE 301 +P Q N DCG+++++Y++ Sbjct: 452 LPMQRNGFDCGMFMVKYID 470 >At2g42220.1 68415.m05225 rhodanese-like domain-containing protein contains rhodanese-like domain PF:00581 Length = 234 Score = 27.1 bits (57), Expect = 7.5 Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 2/54 (3%) Frame = +2 Query: 197 PLKIFNKDNIKGSCLKIPQQNNFTDC--GLYLLQYVEQFFKDPITDYTLPIKQL 352 PL I+N+DN G+ +K NNF+ GL + +F K +++ K L Sbjct: 89 PLFIYNEDNDIGTIIKRTVHNNFSGLFFGLPFTKVNPEFLKSVRNEFSQDSKLL 142 >At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to At5g28270, At2g05450, At1g45090, At2g16180, At2g06750 Length = 1224 Score = 27.1 bits (57), Expect = 7.5 Identities = 22/94 (23%), Positives = 40/94 (42%), Gaps = 1/94 (1%) Frame = +2 Query: 50 MEPQQCCSKNEPIKQPCILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNKDNIK 229 M+P + ++ ++ + I + L R AT +DYL Y V+P Sbjct: 1115 MDPLRSLTRESVVRSRILPIMEMLPYLVR----ATCKDYLDKPY--PVTPFTYIRNQ--- 1165 Query: 230 GSCLKIPQQNNFTDCGLYLLQYVEQF-FKDPITD 328 ++PQ DCG Y ++++E + P+ D Sbjct: 1166 ----RLPQNPTTGDCGPYAMKFIELYMLNTPVED 1195 >At1g45090.1 68414.m05169 Ulp1 protease family protein similar to At5g28270, At2g12100, At2g05450, At2g16180, At2g06750; contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 1210 Score = 27.1 bits (57), Expect = 7.5 Identities = 22/94 (23%), Positives = 40/94 (42%), Gaps = 1/94 (1%) Frame = +2 Query: 50 MEPQQCCSKNEPIKQPCILIFDSLAGASRSRVVATLRDYLTCEYHVKVSPLKIFNKDNIK 229 M+P + ++ ++ + I + L R AT +DYL Y V+P Sbjct: 1101 MDPLRSLTRESVVRSRILPIMEMLPYLVR----ATCKDYLDKPY--PVTPFTYIRNQ--- 1151 Query: 230 GSCLKIPQQNNFTDCGLYLLQYVEQF-FKDPITD 328 ++PQ DCG Y ++++E + P+ D Sbjct: 1152 ----RLPQNPTTGDCGPYAMKFIELYMLNTPVED 1181 >At4g14970.1 68417.m02301 hypothetical protein Length = 1286 Score = 26.6 bits (56), Expect = 9.9 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = +2 Query: 11 DDDEHDKSPLENKMEPQQCCSKNEPIKQPCILIFDSLAGASRSRVVATLR 160 DD K ++N ++ + NE ++QP I+ AGA S LR Sbjct: 699 DDASQRKVSMKNNLKKSKHSDVNEKLRQPTIMDAFKKAGAVMSHSQTQLR 748 >At4g09280.1 68417.m01536 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain Length = 298 Score = 26.6 bits (56), Expect = 9.9 Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 4/41 (9%) Frame = +2 Query: 242 KIPQQNNFTDCGLYLLQYVEQFFK----DPITDYTLPIKQL 352 K+PQ + DCG+Y L+ +E D ITD + ++++ Sbjct: 237 KVPQNHIRGDCGVYALKILEYLLLGVCFDGITDANIQVRRV 277 >At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'auxin-independent growth promoter -related' based on similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 512 Score = 26.6 bits (56), Expect = 9.9 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = -3 Query: 103 YAGLFDWFIFTTTLLRFH 50 Y GL W +F+TT+ FH Sbjct: 450 YEGLLSWEVFSTTVKAFH 467 >At2g33740.2 68415.m04137 copper-binding protein (CUTA) identical to copper-binding protein CUTA GI:12963361 from [Arabidopsis thaliana]; contains Pfam profile: PF03091 CutA1 divalent ion tolerance protein Length = 182 Score = 26.6 bits (56), Expect = 9.9 Identities = 11/35 (31%), Positives = 21/35 (60%) Frame = +3 Query: 180 TMLKYLLSKYSIRTISKGVALKYLSKTILQIVAYI 284 +++ L SK+S + S + ++ SKT+ IV Y+ Sbjct: 51 SVVPLLRSKFSSKAFSSSIRMEESSKTVPSIVVYV 85 >At2g33740.1 68415.m04136 copper-binding protein (CUTA) identical to copper-binding protein CUTA GI:12963361 from [Arabidopsis thaliana]; contains Pfam profile: PF03091 CutA1 divalent ion tolerance protein Length = 156 Score = 26.6 bits (56), Expect = 9.9 Identities = 11/35 (31%), Positives = 21/35 (60%) Frame = +3 Query: 180 TMLKYLLSKYSIRTISKGVALKYLSKTILQIVAYI 284 +++ L SK+S + S + ++ SKT+ IV Y+ Sbjct: 51 SVVPLLRSKFSSKAFSSSIRMEESSKTVPSIVVYV 85 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,986,402 Number of Sequences: 28952 Number of extensions: 184990 Number of successful extensions: 621 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 593 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 615 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 937669760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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