Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32125
(366 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 47 1e-06
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 45 3e-06
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 38 4e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 32 0.024
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 32 0.032
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 26 1.6
SPCC417.11c |||glutamate-1-semialdehyde 2,1-aminomutaseaminotran... 25 4.9
SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb... 24 8.5
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 24 8.5
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 46.8 bits (106), Expect = 1e-06
Identities = 23/69 (33%), Positives = 35/69 (50%)
Frame = +1
Query: 160 SRKAKRTGSNVFSMFSQKQVAEFXEAFQLMDHXKDGIIGXNDLRATFDSLGRLASXKELD 339
++ AKR S F+ + Q+ E EAF L+D DG IG D++ SL + AS ++
Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87
Query: 340 EMVGEASGP 366
M + P
Sbjct: 88 HMFESINPP 96
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 45.2 bits (102), Expect = 3e-06
Identities = 21/50 (42%), Positives = 30/50 (60%)
Frame = +1
Query: 205 SQKQVAEFXEAFQLMDHXKDGIIGXNDLRATFDSLGRLASXKELDEMVGE 354
+ +Q+AEF EAF L D +DG I N+L SLG+ + EL +M+ E
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINE 56
Score = 31.1 bits (67), Expect = 0.056
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +1
Query: 223 EFXEAFQLMDHXKDGIIGXNDLRATFDSLGRLASXKELDEMVGEA 357
E EAF++ D +G I +L SLG S +E+ +M+ EA
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA 130
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 38.3 bits (85), Expect = 4e-04
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = +1
Query: 223 EFXEAFQLMDHXKDGIIGXNDLRATFDSLGRLASXKELDEMV 348
EF + FQ+ D G+IG +LR SLG S +E+DE++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 32.3 bits (70), Expect = 0.024
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = +1
Query: 205 SQKQVAEFXEAFQLMDHXKDGIIGXNDLRATFDSLGRLASXKEL 336
+++Q + EAF+L D KD I ++LRA +LG A E+
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEV 75
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 31.9 bits (69), Expect = 0.032
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = +1
Query: 205 SQKQVAEFXEAFQLMDHXKDGIIGXNDLRATFDSLGRLASXKELDEMVGE 354
S++Q E EAF L D KDG+I + + + SLG + EL ++ E
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNE 53
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 26.2 bits (55), Expect = 1.6
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = +1
Query: 223 EFXEAFQLMDHXKDGIIGXNDLRATFDSLGRLASXKELDEMV 348
E EAF L D G I DLR + LG + ++L M+
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPCC417.11c |||glutamate-1-semialdehyde
2,1-aminomutaseaminotransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 435
Score = 24.6 bits (51), Expect = 4.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 141 QAILSRKPQG*AHRIQCLLYVLTEAGRRVXGGIP 242
+ + SR G +I CL LT G+ + GG+P
Sbjct: 244 EVMTSRLSPGGLQQIYCLKPDLTTLGKYLGGGLP 277
>SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 557
Score = 23.8 bits (49), Expect = 8.5
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -1
Query: 306 GVEGGAQVVLADDAILVV 253
GVE GAQ +++DD LV+
Sbjct: 55 GVESGAQPLVSDDGKLVL 72
>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1364
Score = 23.8 bits (49), Expect = 8.5
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +1
Query: 262 DGIIGXNDLRATFDSLGRLASXKELDEMVGEASG 363
D +IG N R SLG + +EL E A G
Sbjct: 14 DNVIGRNKSRKRPHSLGGPGALQELKEHTNPAKG 47
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.131 0.362
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 947,584
Number of Sequences: 5004
Number of extensions: 10357
Number of successful extensions: 35
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 114084208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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