Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32070
(506 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 32 0.19
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 31 0.34
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 31 0.34
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 31 0.34
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 31 0.59
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 30 0.78
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 30 0.78
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 30 0.78
At5g10020.1 68418.m01161 leucine-rich repeat transmembrane prote... 30 1.0
At3g08870.1 68416.m01031 lectin protein kinase, putative similar... 30 1.0
At2g31400.1 68415.m03837 pentatricopeptide (PPR) repeat-containi... 30 1.0
At3g07250.1 68416.m00863 nuclear transport factor 2 (NTF2) famil... 29 1.4
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 29 1.4
At2g34910.1 68415.m04286 expressed protein 29 2.4
At5g44390.1 68418.m05435 FAD-binding domain-containing protein s... 28 3.1
At5g39710.1 68418.m04808 pentatricopeptide (PPR) repeat-containi... 28 4.2
At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containi... 28 4.2
At1g03060.1 68414.m00280 WD-40 repeat family protein / beige-rel... 27 7.3
At1g12700.1 68414.m01473 helicase domain-containing protein / pe... 27 9.6
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 32.3 bits (70), Expect = 0.19
Identities = 14/18 (77%), Positives = 17/18 (94%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSG 61
V++SAGAI SPQLL+LSG
Sbjct: 273 VILSAGAIGSPQLLMLSG 290
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 31.5 bits (68), Expect = 0.34
Identities = 13/18 (72%), Positives = 17/18 (94%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSG 61
V++SAGA+ SPQLL+LSG
Sbjct: 294 VILSAGALGSPQLLMLSG 311
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 31.5 bits (68), Expect = 0.34
Identities = 14/18 (77%), Positives = 17/18 (94%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSG 61
V++SAGAI SPQLL+LSG
Sbjct: 275 VILSAGAIASPQLLMLSG 292
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 31.5 bits (68), Expect = 0.34
Identities = 15/29 (51%), Positives = 21/29 (72%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSGNWTSQALGRPK 94
V++S+GAI SPQ+L+LSG + L R K
Sbjct: 289 VILSSGAIGSPQMLMLSGIGPKKELQRLK 317
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 30.7 bits (66), Expect = 0.59
Identities = 13/18 (72%), Positives = 16/18 (88%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSG 61
V++SAGA+ SPQLL LSG
Sbjct: 285 VILSAGALGSPQLLFLSG 302
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile
PF00888: Cullin family
Length = 738
Score = 30.3 bits (65), Expect = 0.78
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +1
Query: 304 NSPASDMQYHYLIFPPSSYNLLDMFRKHGLSEEVHDKFREMNE 432
N PA D + + +++ + YN+ H S++++DK+RE E
Sbjct: 30 NEPAFDSEQYMMLYT-TIYNMCTQKPPHDYSQQLYDKYREAFE 71
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 30.3 bits (65), Expect = 0.78
Identities = 12/18 (66%), Positives = 17/18 (94%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSG 61
V+++AGA+ SPQ+LLLSG
Sbjct: 244 VILAAGALGSPQILLLSG 261
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 30.3 bits (65), Expect = 0.78
Identities = 12/18 (66%), Positives = 17/18 (94%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSG 61
V+++AGA+ SPQ+LLLSG
Sbjct: 264 VILAAGALGSPQILLLSG 281
>At5g10020.1 68418.m01161 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase ERECTA,
Arabidopsis thaliana, EMBL:AC004484
Length = 1048
Score = 29.9 bits (64), Expect = 1.0
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = -2
Query: 118 GKSALTSMFRSSKCLRGPIPGEQQQLWGVNSTSGNDNXF 2
G S+L + S GPIPG +LW +N + + N F
Sbjct: 121 GISSLQHLDLSDNGFYGPIPGRISELWSLNHLNLSSNKF 159
>At3g08870.1 68416.m01031 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 693
Score = 29.9 bits (64), Expect = 1.0
Identities = 22/78 (28%), Positives = 35/78 (44%)
Frame = -2
Query: 487 YPQIWVSTKYCKRAWCICSRSFHGTYRGLLRLNRAYGTCPISCRSLEGILSSGTACQKQE 308
Y ++++TK K + I + F YRG L + I+ SL+G+ + E
Sbjct: 358 YRDLYLATKKFKESEIIGTGGFGIVYRGNLSSSGPIAVKKITSNSLQGVRE---FMAEIE 414
Query: 307 SSGRLCFRKR*LCVGWCR 254
S GRL + GWC+
Sbjct: 415 SLGRLGHKNLVNLQGWCK 432
>At2g31400.1 68415.m03837 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 918
Score = 29.9 bits (64), Expect = 1.0
Identities = 21/53 (39%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = +1
Query: 319 DMQYHYLIFPPSSYN-LLDMFRKHGLSEEVHDKFREMNE--NKYTMLVYNTLL 468
+M+Y + SYN LL ++ K G SEE D REM K ++ YN LL
Sbjct: 434 EMRYLGIALDRVSYNTLLSIYTKVGRSEEALDILREMASVGIKKDVVTYNALL 486
>At3g07250.1 68416.m00863 nuclear transport factor 2 (NTF2) family
protein / RNA recognition motif (RRM)-containing protein
contains Pfam profiles PF00076: RNA recognition motif.
(a.k.a. RRM, RBD, or RNP domain), PF02136: Nuclear
transport factor 2 (NTF2) domain
Length = 1294
Score = 29.5 bits (63), Expect = 1.4
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = +3
Query: 75 KHLEDLNIEVKADLPVGVKLAGPFVRT 155
K +ED N+EV+ +L + + L GPF +T
Sbjct: 4 KFVEDENVEVELELELCLSLGGPFKKT 30
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 29.5 bits (63), Expect = 1.4
Identities = 11/18 (61%), Positives = 16/18 (88%)
Frame = +2
Query: 8 VVVSAGAINSPQLLLLSG 61
+++SAG + SPQLL+LSG
Sbjct: 273 IILSAGTLGSPQLLMLSG 290
>At2g34910.1 68415.m04286 expressed protein
Length = 288
Score = 28.7 bits (61), Expect = 2.4
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1
Query: 247 DLIDTSPHRVIAFENTTDPNSPAS 318
DL +TSP R + F TT + PAS
Sbjct: 240 DLAETSPQRRVRFSTTTSDSCPAS 263
>At5g44390.1 68418.m05435 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 542
Score = 28.3 bits (60), Expect = 3.1
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +1
Query: 130 NLQDHLFVPVFYTKPGDKKATTLPNIISTFIEYFLHNTGDLID 258
NL D LF+ VF+T G+K T + +I FL G L++
Sbjct: 289 NLVDELFLRVFFTVSGNKANKT---VTMAYIGQFLGEKGTLME 328
>At5g39710.1 68418.m04808 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 747
Score = 27.9 bits (59), Expect = 4.2
Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +1
Query: 364 LLDMFRKHGLSEEVHDKFREMNENKY--TMLVYNTLL 468
L+D F + G E + REMN+N + +++ YN L+
Sbjct: 386 LVDGFSQKGYMNEAYRVLREMNDNGFSPSVVTYNALI 422
>At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 650
Score = 27.9 bits (59), Expect = 4.2
Identities = 15/42 (35%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
Frame = +1
Query: 355 SYN-LLDMFRKHGLSEEVHDKFREMNENKYT--MLVYNTLLK 471
SYN L++ K+G +E H +F+EM E ++ Y+TL++
Sbjct: 514 SYNSLINCLGKNGDVDEAHVRFKEMQEKGLNPDVVTYSTLME 555
>At1g03060.1 68414.m00280 WD-40 repeat family protein /
beige-related similar to BEIGE (GI:3928547) [Rattus
norvegicus]; Similar to gb|U70015 lysosomal trafficking
regulator from Mus musculus and contains 2 Pfam PF00400
WD-40, G-beta repeats. ESTs gb|T43386 and gb|AA395236
come from this gene
Length = 3601
Score = 27.1 bits (57), Expect = 7.3
Identities = 17/56 (30%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
Frame = -3
Query: 291 VFESDNSVWAGVD*VASIVQEV-FNESRDNIRQGSSFLVSRFGIEDGYEQMVLQVL 127
VFE DN++ G D + S++++ N+S G + ++ F I D + VL++L
Sbjct: 681 VFEVDNTITVGWDCLISLLKKAEANQSSFRAANGVAIILP-FLISDAHRSGVLRIL 735
>At1g12700.1 68414.m01473 helicase domain-containing protein /
pentatricopeptide (PPR) repeat-containing protein
contains Pfam profiles PF01535: PPR repeat, PF00271:
Helicase conserved C-terminal domain
Length = 828
Score = 26.6 bits (56), Expect = 9.6
Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 2/37 (5%)
Frame = +1
Query: 364 LLDMFRKHGLSEEVHDKFREMNENKYT--MLVYNTLL 468
LLD+F K G +E ++ ++EM + ++ YNTL+
Sbjct: 294 LLDVFVKEGKLQEANELYKEMITRGISPNIITYNTLM 330
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,516,039
Number of Sequences: 28952
Number of extensions: 239122
Number of successful extensions: 696
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 696
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 908059136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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