Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= epV32049
(516 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 69 4e-13
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 62 4e-11
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 31 0.077
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 28 0.95
SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomy... 25 5.1
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 25 6.7
SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr 1|... 25 6.7
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 25 6.7
SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po... 25 6.7
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 68.9 bits (161), Expect = 4e-13
Identities = 39/124 (31%), Positives = 64/124 (51%), Gaps = 1/124 (0%)
Frame = +2
Query: 38 TQSHNMSLERQVRAKIASKRNPXKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLI 217
T+ N L + R + + + EA++WIE LG P FE L++G VL L+
Sbjct: 20 TKGSNTRLAAKQRETLQAYDYLCRVDEAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLV 79
Query: 218 NKLKPGSVPKINTTGG-QFKMMENITNFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVST 394
K +P + KI + QF+ +NI F I G+P+I F+ D++E K++ +V+
Sbjct: 80 QKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYC 139
Query: 395 LFAL 406
+ AL
Sbjct: 140 IHAL 143
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 62.5 bits (145), Expect = 4e-11
Identities = 41/127 (32%), Positives = 65/127 (51%), Gaps = 1/127 (0%)
Frame = +2
Query: 110 EKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENI 289
EKEA+EWIE L K D L+ G +LC++ + ++ + + F MENI
Sbjct: 6 EKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQMENI 64
Query: 290 TNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLGPKPA 466
+ F A + VP D+FQT DL+E+++ QV+ ++ + R + LGPK A
Sbjct: 65 SAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLA 124
Query: 467 DECKRDF 487
++ R F
Sbjct: 125 EKKPRVF 131
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 31.5 bits (68), Expect = 0.077
Identities = 28/101 (27%), Positives = 49/101 (48%), Gaps = 4/101 (3%)
Frame = +2
Query: 116 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 295
EA++W+E ++ + F D L +G VLCQL K P + ++++ E T
Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTV 125
Query: 296 FQSA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 406
+ +A + G+ F+T DL + +I +V+ L AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166
>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 992
Score = 27.9 bits (59), Expect = 0.95
Identities = 18/48 (37%), Positives = 22/48 (45%), Gaps = 4/48 (8%)
Frame = -1
Query: 234 PGFSLLMSWQR----TVPSLRTSSNSSPGGNLAPRTPSIHSWASFSXS 103
PG L QR T P L TSS++ PGG + + HS S S
Sbjct: 210 PGVGTLQQPQRAGSDTFPDLNTSSSNQPGGEPNVASANTHSLEILSSS 257
>SPAC13G7.03 |||up-frameshift suppressor3 family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 278
Score = 25.4 bits (53), Expect = 5.1
Identities = 19/80 (23%), Positives = 34/80 (42%)
Frame = +2
Query: 29 KALTQSHNMSLERQVRAKIASKRNPXKEKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLC 208
K ++ S++ + + ++A K K + E +K P E V+K+ V
Sbjct: 166 KGKSKPSKKSVKAKKKLRLAEKPASNNSKAGKSSQESKKSSK-APAESAAAVIKEDKVSD 224
Query: 209 QLINKLKPGSVPKINTTGGQ 268
+ +K KP P N+T Q
Sbjct: 225 RKKSKKKPKKTPVSNSTASQ 244
>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 589
Score = 25.0 bits (52), Expect = 6.7
Identities = 10/45 (22%), Positives = 20/45 (44%)
Frame = +1
Query: 241 PQDQHHRWTVQNDGEHHKLPVCNQSIRCTRHRCVPNRRSMGEKRH 375
P+ +++++ +HHKL + RC P +K+H
Sbjct: 206 PRSSTTSYSIRDADKHHKLTTSGVTKMNITERCKPKTTIQTDKKH 250
>SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 376
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = -3
Query: 478 FAFVSGLGSEAGAAPFRVSVSLTTEGKQCADYLGDVFFL 362
FAFVS EA + F ++ G Q + + +FF+
Sbjct: 51 FAFVSEKSLEAASVKFVATIIAERMGAQFSSFYDKIFFV 89
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 25.0 bits (52), Expect = 6.7
Identities = 25/87 (28%), Positives = 39/87 (44%), Gaps = 10/87 (11%)
Frame = +2
Query: 143 LGAKFPPG-ELFE--DVLKDGTVLCQLINKLKPGSV-------PKINTTGGQFKMMENIT 292
+G++ P E FE D KDG +L +LIN P ++ + N FK +EN
Sbjct: 132 VGSRVPINTETFEFFDQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNN 191
Query: 293 NFQSAIKAYGVPDIDVFQTVDLWEKKD 373
++ KA G I D+ E ++
Sbjct: 192 VVINSAKAMGGISITNIGAGDILEGRE 218
>SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1159
Score = 25.0 bits (52), Expect = 6.7
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -3
Query: 466 SGLGSEAGAAPFRVSVSLTTEGKQ 395
SG G+ AG++PF S +LT E K+
Sbjct: 656 SGFGNTAGSSPF--SFNLTKESKE 677
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,241,543
Number of Sequences: 5004
Number of extensions: 48238
Number of successful extensions: 139
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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